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Biomedical subjects

G R Smith

Publications and source records attributed to G R Smith.

At least 361 records · Page 20Linked to original sources
PubMed1971

Nature and self-regulated synthesis of the repressor of the hut operons in Salmonella typhimurium.

The enzymes mediating histidine utilization, coded by the hut genes, in Salmonella typhimurium are synthesized from two closely-linked operons. Results presented in this paper show that these operons are regulated by a single repressor protein. The hutC gene, coding for this repressor, is part of one of the operons. Since this same operon is sensitive to the repressor, the repressor apparently represses its own synthesis.

Acrylates↗
PubMed1971

Adenosine 3':5'-cyclic monophosphate concentration in the bacterial host regulates the viral decision between lysogeny and lysis.

Mutants of Salmonella typhimurium defective in adenylate cyclase (cya gene) or in cAMP receptor protein (crp gene) are lysogenized at reduced frequency by phage P22. One class of the bacterial mutants with an altered RNA polymerase (rif gene) is also lysogenized at reduced frequency. In the three types of mutant bacteria, the phage's decision between lysogeny and lysis is shifted to lysis and the phage form clear plaques. We propose that in wild-type bacteria the cAMP-receptor protein, in combination with cAMP, activates bacterial RNA polymerase to transcribe certain phage genes that are required for efficient lysogenization. Under conditions of strong catabolite repression, when the supply of energy and biosynthetic components is abundant and the concentration of cAMP is low, the phage would multiply and lyse the cell. When the supply of energy is deficient and the concentration of cAMP is high, the phage would lysogenize the cell. Phage mutants have been isolated that form turbid plaques on the three classes of bacterial mutants due to a higher frequency of lysogeny. These phage mutants have been shown by complementation to be defective in the same gene, which we have called the cly gene. These cly mutants lysogenize the wild-type bacteria with a 99% frequency and, thus, do not form plaques on them. Other kinds of bacterial mutants are also lysogenized at reduced frequency by phage P22. They may be altered in other physiological control systems that influence the frequency of lysogenization.

Adenylyl Cyclases↗
PubMed1967

Causal basis of gravity stimulus nullification by clinostat rotation.

Using appropriate clinostat rotation methods, it has been shown that increases in root growth and geotropic curvature of oat coleoptiles are related to and explained (within the limits of auxin economy) by increased rates of plant respiration imparted by nullification of the gravitational stimulus. Increased respiration with decreased gravitational stimulus, in turn, is explained by increased uniformity in intracellular distribution of metabolically active graviprecipitable particles.Oat seedlings grown with nullification of the gravitational stimulus show a more uniform distribution of graviprecipitable protoplasmic inclusions. Respiration involves reactions between particles of sufficient mass to be precipitated in 1 x g unidirectional force field and particles of such size that they are distributed throughout the protoplasm due to thermal energy. When plants are grown without nullification of the gravitational stimulus, graviprecipitable protoplasmic inclusions tend to accumulate in the lower portions of the cells. Respiration rates are consequently limited due to a relatively low concentration of the smaller particles in the region of high concentration of the larger particles. Distribution of the larger particles throughout the protoplasm negates the reaction rate limiting effects of these intracellular concentration gradients.It has been shown using starch particles to index graviprecipitation of intracellular particles that enhancement of respiration by nullification of the gravitational stimulus may be induced, maintained, discontinued, and reinduced by treatments which concomitantly result in the more uniform distribution of graviprecipitable protoplasmic inclusions, the maintenance of this uniform distribution, the discontinuation of this uniform distribution and its reinduction.The degree of uniformity of intracellular particle distribution in response to treatments only partially nullifying the gravitational stimulus is shown wholly consonant with growth responses of roots and coleoptiles when the growth is measured as the expression of increased respiration in the absence of auxin limitation.The findings collectively indicate that gravity sensing by plants is broadly based on the physicochemical relationship between particle distribution and the expression of respiratory metabolism as growth.

Journal Article↗
PubMedDate not supplied

Healthy patients who perceive poor health and their use of primary care services.

OBJECTIVE: To determine what proportion of patients who have poor health perceptions are physically healthy and to explore why some patients perceive a healthy state while others perceive illness. DESIGN: A prospective consecutive series of office patients completed the Rand Corporation's General Health Perceptions Questionnaire, and their physicians rated their physical health. Their use of health care services was determined for the following 12 months. SETTING: A rural teaching office practice. PATIENTS: Of 243 adult patients asked to complete the questionnaire, 32 were excluded, for dementia (8), illiteracy (4), illness (8), incomplete questionnaires (6), and other reasons (6). 208 patients (86%) formed the final study group. MEASUREMENTS AND MAIN RESULTS: 62 of 208 patients had poor health perception scores. 39 of the 62 were rated by physicians as physically healthy and were not statistically different in physical health ratings or numbers of prescribed medications from the 146 patients who had higher health perception scores. However, these 39 patients had significantly more health-related worry, acute pain, and depression than did the other 146 patients. They also made more office visits and telephone calls and had higher total primary care charges. CONCLUSIONS: This study suggests that 21% of adult primary care patients (39 of 208) have health perceptions lower than expected for their levels of physical health. These low health perceptions are correlated with increased emotional distress and higher utilization of health care resources. Strategies to identify these patients and interventions to improve their views of their health could reduce utilization.

Attitude to Health↗