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Biomedical subjects

G R Moore

Publications and source records attributed to G R Moore.

At least 181 records · Page 10Linked to original sources
PubMed1977

Nuclear-magnetic-resonance studies of Pseudomonas aeruginosa cytochrome c-551.

Nuclear magnetic resonance (NMR) spectroscopy was used to study Pseudomonas aeruginosa cytochrome c-551. Assignments of resonances to specific residues have been made. A low-resolution X-ray structure was used to aid assignments. A structural comparison was made between P. aeruginosa cytochrome c-551 and mammalian cytochrome c, based on comparisons of NMR data.

Amino Acids↗
PubMed1975

Sinusitis of the maxillary antrum.

Twenty-four adults with clinical evidence of sinusitis were studied by 65 needle punctures of the maxillary antrum. Fourteen of 15 sinuses with normal transillumination and 19 of 26 that were dull had normal aspirates, whereas 24 of 24 that were opaque had abnormal aspirates (P less than 0.001). Marked mucosal thickening as determined radiologically (Water's view) was associated with abnormal aspirates whereas lesser mucosal thickening was not (P less than 0.001). In acute sinusitis, there was a strong correlation between high aspirate leukocyte counts (greater than 1000 per cubic millimeter) and infection as manifested by bacterial titers of greater than 10(5) per milliliter or the isolation of virus fungus (P greater than 0.001). Anterior-nasal-swab cultures correlated poorly with direct aspirate culters. Organisms frequently recovered from the sinus included Haemophilus influenzae, Strepto coccus pneumoniae, and anaerobic bacteria. Rhinovirus was recovered twice. Antibiotics were usedful in patients with acute sinusitis if the organism was sensitive in vitro (P less than 0.001).

Acute Disease↗
PubMedDate not supplied

Isolation and properties of the complex nonheme-iron-containing cytochrome b557 (bacterioferritin) from Pseudomonas aeruginosa.

A nonheme-iron-containing cytochrome b557, also known as bacterioferritin, was isolated from Pseudomonas aeruginosa. Gel electrophoresis showed that the protein subunits had a molecular weight of 18 kD and it is suggested that the intact molecule contained 24 subunits. The isolated protein contained 8.7% by weight Fe and 8% by weight phosphate. Most of the Fe was contained in the nonheme-iron core and could be readily removed by dialysis against 0.12 M thioglycollic acid. The resulting apobacterioferritin contained approximately one protoporphyrin IX group for every five subunits and, in addition, an unidentified fluorophor. The nonheme-iron core was found to be amorphous with a mean core size of 60-65 A.

Bacterial Proteins↗
PubMedDate not supplied

Haem binding to ferritin and possible mechanisms of physiological iron uptake and release by ferritin.

Haem binding to horse spleen ferritin and Pseudomonas aeruginosa bacterioferritin has been studied by spectroscopic methods. A maximum of 16 haems per ferritin molecule, and 24 haems per bacterioferritin molecule, has been shown to bind. The influence of the bound haem on the rate of reductive iron release has been investigated. With a range of reductants and in the absence of haem the rate of release varied with the reductant, but in the presence of haem the rate was both independent of the reductant and faster than with any of the reductants alone. This indicates the rate-limiting step for iron release in the absence of haem was electron-transfer across the protein shell. Based on the results obtained with the in vitro assay system and from a consideration of data currently in the literature, plausible schemes for ferritin and bacterioferritin iron uptake and release are described.

Animals↗
PubMedDate not supplied

Leptomeningeal and adventitial gliosis as a consequence of chronic inflammation.

Astroglial cell bodies and processes identified ultrastructurally and immunocytochemically are described within the subarachnoid and Virchow-Robin spaces of white matter throughout the CNS of Strain 13 guinea-pigs with chronic relapsing experimental allergic encephalomyelitis. These heterotopic astroglial elements, invariably in close proximity to subpial chronically-demyelinated lesions, were derived from subpial astrocytes and extended for great distances perpendicular and circumferential to the underlying white matter. Subpial astrocytes overlying adjacent grey matter regions appeared unaffected. This phenomenon is probably a subpial astrocyte response to inflammation and demyelination and may be related to the protection of damaged white matter against subsequent inflammatory events.

Animals↗