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Biomedical subjects

G Pompa

Publications and source records attributed to G Pompa.

32 records · Page 2Linked to original sources
PubMed1992

Elimination of PCB congeners via milk in rabbits administered Fenclor 64.

A single dose of 100 mg per kg body weight of a commercial mixture of polychlorinated biphenyls (PCB), Fenclor 64 was given intraperitoneally to pregnant rabbits. The distribution in dams and foetuses and excretion in milk was investigated for six of the congeners by quantifying them in fat from maternal adipose tissue, from whole foetuses and newborn bodies and from newborn gastric contents. The cytochrome-P-450 induction after Fenclor 64 in foetuses and suckling off-spring was followed by measuring the following hepatic mixed function oxidase (MFO) activities: p-nitroanisole-demethylase, ethoxyresorufin-deethylase, ethoxycoumarin-deethylase and NADPH cytochrome-C reductase. At the 28th day of pregnancy PCB fat concentrations in foetuses were similar to those in mothers (126.4 +/- 7.1 and 152.6 +/- 28.1 micrograms/g of fat, respectively). By the 5th day of life fat concentrations in the youngs were double those of foetuses (216.81 +/- 8.12 micrograms/g) and remained high until weaning (142.2 +/- 15.5 micrograms/g at the 20th day). PCB concentrations in mothers' fat decreased during lactation (104.1 micrograms/g at the 20th day) but at the end of the experiment they were still high (95.5 micrograms/g). The cytochrome-P-450 concentration and MFO activities in young rabbits' livers from treated dams were significantly higher than controls from the 5th (P less than 0.01) to the 10th (P less than 0.01) day of life, with the exception of NADPH-cyt-C-reductase (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1989

[Varicose veins of the lower limbs caused by incompetence of the saphenous-femoral ostium: standard or complete stripping?].

The Authors report their experience of 256 stripping operations performed during the last 3 years for varicose veins of the lower extremities. Preoperative Doppler examination was used to detect reverse flow in the saphenous veins. Removal of the entire saphenous trunk was performed in 158 limbs with segmental or total reverse flow; in the remaining 98 cases with reflux only in the proximal tract of the saphenous vein a selective stripping operation and, five weeks later, phlebectomy of remaining varicosities were performed. The results were assessed by follow-up examination from 6 months to 3 years. When there is a specified indication, the selective stripping operation appears to be an excellent and aesthetic solution with a very low incidence of saphenous nerve's injuries. Useful advice to obtain the best results with the traditional stripping operation are also pointed out.

Female↗
PubMed1988

Zearanol metabolism by subcellular fractions from lamb liver.

Zeranol (7-alpha-zearalanol or alpha-ZAL) metabolism in vitro by subcellular fractions (microsome and cytosol) from lamb livers was investigated. The use of a high performance liquid chromatography (HPLC) technique capable of resolving epimers, revealed that when nicotinamide adenine dinucleotide (NAD) was added as a co-factor to metabolic mixtures, the oxidized form, zearalanone (ZAN) was the major metabolite, although a small amount of 7-beta-zearalanol (beta-ZAL) was also produced. In order to confirm beta-ZAL as a product of ZAN reduction, the metabolism of the latter, by microsome and cytosol in the presence of NADH as co-factor, was investigated. The results obtained revealed that both alpha-ZAL and beta-ZAL were present at the end of the incubation, the former at a higher concentration than the latter. When NAD and NADH were added as cofactors to incubation mixtures containing alpha-ZAL, the production of beta-ZAL was increased as a consequence of the higher ZAN reduction in the presence of the reducing co-factor. Nevertheless, ZAN remained the major metabolite produced from alpha-ZAL by both the subcellular fractions investigated.

Animals↗
PubMed1987

Effect of PCB on some MFO enzyme activities in pregnant and virgin rabbits.

Fenclor 64 (a PCB's commercial mixture) was administered twice i.p. to virgin and pregnant New Zealand rabbits at the dose of 100 mg/Kg to evaluate its inducing properties on the following hepatic microsomal MFO activities: p-nitro-anisole-O-demethylase, amino-pyrine-N-demethylase, acetanilide-hydroxylase and aryl-hydrocarbons-hydroxylase. The results indicate that Fenclor 64 evoked a "mixed type" induction in virgin as well as in pregnant rabbits since it increased liver weight, cytochrome P-450 levels, as well as p-nitro-anisole-O-demethylase and acetanilide-hydroxylase. Pregnancy by itself affects only amino-pyrine-N-demethylase levels.

Aminopyrine N-Demethylase↗
PubMed1986

The metabolism of zearalenone in subcellular fractions from rabbit and hen hepatocytes and its estrogenic activity in rabbits.

The in vitro reduction of zearalenone (ZEN) by subcellular fractions from hen and rabbit hepatocytes clearly shows species-specific differences in the cofactor requirements, rate of metabolism and production of metabolites. The presence of NADH as cofactor in the reaction mixtures enhanced only the reducing activity of the microsomal fraction from rabbit hepatocytes, while NADPH enhanced the reducing activities of the cytosolic fraction from rabbit and both the microsomal and cytosolic fractions from hen hepatocytes. Furthermore, we observed that hen hepatocytes metabolize faster and produce beta-zearalenol (ZEL) as the major metabolite, whereas rabbit hepatocytes metabolize ZEN slowly and mainly into alpha-ZEL, the more uterotrophic metabolite. These last findings are closely related to the higher sensitivity to ZEN estrogenic effects observed in rabbits during the toxicity test involving p.o. administration of the mycotoxin to the animals at 3 dosage levels (0.1, 1, 2 mg/kg body wt).

Animals↗
PubMed1986

A rapid method for the simultaneous detection by collisionally activated dissociation mass-analysed ion kinetic energy spectrometry of parts per trillion of diethylstilbestrol, zeranol and trenbolone in plasma and edible tissues of cattle.

The current methods for the control of the illegal use of anabolics in cattle breeding are far from satisfactory in terms of specificity and sensitivity. Here we describe a method for the simultaneous detection of diethylstilbestrol, zeranol and trenbolone in plasma and edible cattle tissues. The method is rapid (total analysis time 30 min), specific and sensitive (ppt level). The technique employed is a tandem mass spectrometric method: collisionally activated decomposition mass-analysed ion kinetic energy (CAD MIKE) spectrometry. Detection involves the injection of the crude ethanol extracts of plasma and tissues and selection/collision of molecular ions of each targeted compound. Confirmation of the presence of particular compounds is made by matching the complete CAD spectra so obtained with those of the pure compounds.

Animals↗
PubMed1980

[Squamous metaplasia of the cervical canal: histologic observations concerning 1500 hysterectomies].

1.500 cases of total hysterectomies were examined for the sake of point out the incidence and distribution of squamous metaplasia of the endocervix. It was found in 12,6% of cases with a prevalently focal distribution; the prevalent hystological type was hyperplasia of reserve-cells (78,4%). Essential variations in women over 50 years were not noted. S.M. was associated in 33,7% of cases with adenomyosis and in 37,3% of cases with myomas.

Adenoma↗
PubMedDate not supplied

[Adenomatoid hyperplasia of the minor salivary glands. Report of a clinical case].

The authors describe a case of adenomatoid hyperplasia of a minor salivary gland. On the basis of the literature, they discuss the clinical, diagnostic and therapeutic aspects and dwell upon the value of the histologic examination as the only way to achieve a correct diagnosis, because the lesion cannot be clinically discriminated from the tumoral lesions.

Adenoma↗