Contraception with LHRH agonists, a new physiological approach.
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Animals↗
Biomedical subjects
G Pelletier
Publications and source records attributed to G Pelletier.
[Anti-HBc IgM antibodies: prevalence during HB virus infection and value of studying titers].
IgM antibody to hepatitis B core antigen (IgM anti-HBc) was determined in acute and chronic hepatitis B by the immunocapture method. To avoid interference with the rheumatoid factor, F (ab')2 antibodies were used; non-specific reaction of IgG anti-HBc with anti-mu usually observed with 1/100 dilution of the serum was avoided by 1/1,000 dilution. IgM anti-HBc was positive in 100 p. 100 of the patients with acute hepatitis B (n = 32), in respectively 100 p. 100 and 70 p. 100 of the patients with complete recovery 6 and 12 months after acute illness (n = 10 and n = 10), 20.8 p. 100 of the healthy chronic HBs Ag carriers (n = 48), 80 and 85.7 p. 100 of the patients with chronic persistent and chronic active hepatitis (n = 40 and n = 14). S/N ratios (S = sample, N = negative controls) were above 5 in all patients with acute hepatitis. In patients with complete recovery, 6 to 12 months after acute illness, and in healthy chronic HBs Ag carriers, S/N ratios were above 5 in only 5 and 2 p. 100 of cases respectively whereas in patients with chronic hepatitis, the S/N values were dispersed. The main interest of the IgM anti-HBc test is to allow for the diagnosis between acute hepatitis B and acute hepatitis non B in healthy chronic HBs Ag carriers. In our patients, a S/N ratio above 5 discriminated between an acute hepatitis B and a healthy chronic HBs Ag carrier with a specificity of 98 p. 100.
Carrier State↗
[Ascitic pH and infection in alcoholic cirrhosis].
The pH values of 108 samples of ascitic fluid in 94 alcoholic cirrhotic patients were analyzed in order to assess their diagnostic and prognostic value. The mean pH value of ascitic fluid was significantly lower (p less than 0.001) in patients with spontaneous bacterial peritonitis (7.23 +/- 0.22) or with suspected diagnosis of spontaneous bacterial peritonitis (7.29 +/- 0.15) than in patients with sterile ascites (7.45 +/- 0.06). However, there was an important overlap between these groups. In patients with and without spontaneous bacterial peritonitis, measurement of the difference between blood and ascitic pH was more discriminative than the ascitic pH alone: a difference of 0.10 or more was detected in all patients with spontaneous bacterial peritonitis, in 2 of 5 patients with suspected diagnosis of spontaneous bacterial peritonitis and in 3 of 97 patients with sterile ascites. When the ascitic pH value was lower than 7.15, death occurred rapidly. Ascitic pH rapidly increased when treatment of spontaneous bacterial peritonitis was clinically effective. These results suggest that measurement of pH in ascitic fluid is contributive to the diagnosis and prognosis of spontaneous bacterial peritonitis in alcoholic cirrhosis.
Adult↗
Ontogeny of IGFs and IGFBPs mRNA levels and tissue concentrations in liver, kidney and skeletal muscle of pig.
As far as we know, there is no available information about ontogenic changes of tissue concentrations of IGF-I and II and IGFBPs in large mammals. Serum, liver and kidney levels of IGFs and IGFBPs were examined in fetuses at 90 and 110 days of gestation and in pigs at 1d, 3 wk, 3 mo and 6 mo of age. Ontogeny of mRNA levels of IGFs, IGF type I and type II receptors (IGFI-R and IGFII-R), IGFBP-1 and -3 (IGFBPs) and growth hormone receptor (GHR) were also examined by Northern blot analysis in liver, kidney and skeletal muscle of pig. Serum IGF-I, IGF-II and IGFBP-3 concentrations were low during the fetal life and increased after birth. The highest level of IGF-II mRNA was found in fetuses for all studied tissues. In the liver, IGF-I mRNA level and its protein content peaked at 3 wk of age. The highest IGF-II concentration was found at 1d and 3 wk of age. The IGFII-R mRNA remained at a constant level during the whole development period. The most abundant IGFBP-1 mRNA and its protein content were found at birth. The level of IGFBP-2 was high during fetal and early postnatal life. The IGFBP-3 content was relatively low in fetuses and reached the highest level after 3 wk of age. In the kidney, IGFs, IGFBP-3, IGFI-R and IGFII-R as well as GHR mRNA levels were relatively high during the fetal and early postnatal life. The IGFs concentrations were the highest in newborns. In the skeletal muscle, IGFs, IGFBP-3 and IGFI-R mRNA levels decreased with advancing age. During the postnatal life, the high IGFs concentrations in the liver and the kidney correspond to fast growth periods of these organs.
Animals↗
Transcriptional regulation of the alpha 4 integrin subunit gene in the metastatic spread of uveal melanoma.
Recently, expression of the alpha 4 integrin subunit has been shown to be inversely correlated with the invasive potential of B16 mouse epidermal melanoma. The purpose of this study was to establish whether expression of the human alpha 4 integrin subunit gene might be similarly regulated in human uveal melanoma which has varying degrees of invasiveness, and whether such modifications are determined by alterations in the transcriptional activity directed by the alpha 4 gene promoter. Two metastatic variants (MH5 and MH10) derived from a human uveal melanoma (SP6.5) were used. Expression studies were performed by transiently transfecting each of these cell lines with recombinant plasmids bearing various lengths of the alpha 4 promoter fused to the CAT reporter gene, and were further validated by Northern blot analyses of the alpha 4 transcript. Both transient transfection and mRNA analyses provided evidence that the transcriptional activity directed by the alpha 4 promoter sequences extending up to position -76 and -120 was indeed inversely correlated to the potential of uveal melanoma to yield metastasis. Experiments in electrophoretic mobility shift assay (EMSA) demonstrated that binding of the nuclear proteins that likely account for transcription of the alpha 4 gene to alpha 4.1 (namely Bp1, Bp2, Bp4, and Bp5) was dramatically reduced in uveal melanoma, but not in normal uveal melanocytes. These results highlight the fundamental function the alpha 4 integrin subunit may play in the ability of tumor cells to evade the primary tumor and form metastasis.
Adolescent↗