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Biomedical subjects

G Paoli

Publications and source records attributed to G Paoli.

At least 37 records · Page 2Linked to original sources

[Imposing "calcium milk" lithiasis in giant hydronephrosis].

The authors report a quite unusual case of "milk of calcium renal Stone" in unilateral giant Hydronephrosis. Radiographic, sonographic, intravenous urographic and TC aspects are described and prominence il given to morphostructural information obtained through digital image techniques of pyeloureteral zone junction and of "milk of calcium renal Stone". The authors discuss the pathogenesis of the renal Stone type and emphasize the convenience of investigative acts to clear up the ethio-pathogenetic role of pyeloureteral infections.

Adult↗

Isolation factors and kinship by isonymy in a group of parishes in northern Tuscany (Italy): influence of within-parish similarity level on between-parish similarity pattern.

We investigate the influence of within-parish similarity level on between-parish similarity pattern in surname analysis through a study of the relative roles of various isolation factors accounting for the population structure within a municipality (21 parishes) in the hilly part of northern Tuscany. One surname per family was collected (N = 3052), and the pattern of kinship observed by isonymy was compared with the expected patterns based on demogeographic variables. The fit of the isolation by distance model is substantially improved with the inclusion of values at zero geographic distance (i = j). Differentiation in migration rate was clearly shown by the local kinship values, which are highly correlated with the demogeographic features of the parishes under study. Furthermore, the strong heterogeneity of the local kinship values seems to be the major factor responsible for the poor relation of the off-diagonal values of the kinship matrix to the expected isolation pattern. In fact, the kinship matrix weighted on the basis of the local kinship values points to a pattern of similarity among parishes congruent with the demogeographic structure of the population. On the whole, the results indicate that at the low hierarchical level of parishes the extreme localization of surnames is a disturbing factor in population structure analysis. A simple method to overcome this problem has been proposed.

Altitude↗

Numerical method for the interpolation of digitized lines (unrolling method).

The proposed method can be used for reconstructing, from n not necessarily equidistant points of a digitized polydrome line, a set of n1 equidistant points (with n1 > n) interpolating the original points. This method is based on a transformation of the original line into a digitized monodrome function D(1i) (unrolling function), where 1i is the line length between the origin and the ith point (i = 1,..., n1). Advantage of this method consists in reducing the two-dimensional interpolation problem to the one-dimensional field. In scintigraphic imaging, it is possible to achieve interpolation and coding of ROI's (Regions Of Interest) edges. For n samples of a monodrome line this method can also be applied, representing a possible alternative to splines. The numerical procedure is developed to reduce the noise on the points.

Heart↗

Paleoserology of the Christian population at Sayala (Lower Nubia): an evaluation of the reliability of the results.

A paleoserological study of human remains from the Christian cemeteries of Sayala (Lower Nubia, 6th-11th centuries A.D.) was carried out by applying two techniques (absorption-elution and haemagglutination-inhibition), on two types of substratum (bones and hair), in separate laboratories (Pisa and Prague). The aim of research was to evaluate the degree of repeatability of the results and the reliability of the final paleoserological diagnoses. The results indicated different degrees of repeatability depending on the differential sensitivity of the two techniques and on the nature of the substratum. The frequencies of concordant and discordant final diagnoses within and between the two laboratories and by the two methods were significantly different from those expected under the conditions of random distribution, indicating a consistency in results from different laboratories and methods. The reliability of the resulting gene and phenotype distributions is also supported by their consistency both with the theoretical Hardy-Weinberg equilibrium and with the present distribution of the ABO gene frequencies in Egyptian, Sudanese, and Nubian populations.

Blood Grouping and Crossmatching↗

Variability of DA/DAPI and C heterochromatic regions: a population study.

DA/DAPI and C chromosomal heteromorphic sites (1q, 9q, 15p, 16q, Yq) in a sample of 136 unrelated individuals from the Garfagnana valley (Tuscany, Italy) have been analyzed quantitatively and qualitatively. The variations in length, between-homolog heteromorphisms, and intensity of fluorescence of the heterochromatic bands have been compared in two subsamples of the population (upper and middle valley) individualized according to geodemographic criteria. DA/DAPI heterochromatin differed significantly from C heterochromatin, showing a lower average amount and a higher variability at each site. This suggests a differential staining of DNA of the two banding systems. Furthermore, DA/DAPI heterochromatin was less uniformly distributed in the population than C heterochromatin and the regions 16q and Yq discriminated better between subsamples. The variations of DA/DAPI fluorescence at the 15p site demonstrated an excess of homomorphic individuals in the upper valley, which could be related to the mating structure of the population living in this area.

Adolescent↗

Genetic polymorphism in the Garfagnana population (Tuscany).

Blood group systems ABO, KEL, MN, RH, secretor status for ABH blood group substances, red cell enzymes ACP1, ESD, AK1, 6-PGD, PGM1 subtypes, serum markers GC, TF, PI, AHSG, ORM1 and chromosomal heteromorphism Q, C, DA/DAPI were examined in a population sample of the Garfagnana, a semi-isolated mountainous area in the province of Lucca (Tuscany, Italy). The total sample was subdivided and analysed according to the more recent historical events which have occurred in Garfagnana. The observed phenotype and gene frequencies were discussed in the context of other population samples from the same province and of the same region. As a whole, the results concur in pointing out a certain degree of heterogeneity within the area and a high differentiation from the rest of Tuscany. Such peculiar genetic features of the population have to be related to the historical, geographic and cultural isolation of the Garfagnana.

Blood Group Antigens↗

Surname versus gene structure of a small isolate.

A hierarchical partition of the Wright coefficient of inbreeding (F) is shown for an isolated population (about 2000 inhabitants) from a mountainous region of Tuscany (Italy) with regard to both surnames and genes. At total population level surname variation proves substantially consistent with the observed genetic differentiation of the isolate. Analysis of parental isonymy reveals that the population is structured in subunits and it is concluded that the unit population of the region may be very small, practically coinciding with the village (less than 500 inhabitants). The agreement of 'demographic' and genetic structures is less satisfactory at this level, partly because of subsample size, but we conclude that analysis of surnames may give at least a rough idea of the genetic structure of the population.

Consanguinity↗

Genetic studies in the Garfagnana population (Tuscany, Italy).

The relationship between geographic isolation and historical-demographic features and genetic structure and pattern of variation of genetic markers was analyzed in the population of Garfagnana, a semi-isolated mountainous area in the province of Lucca (Italy), taking into account hierarchical subdivisions. A random sample of unrelated individuals, whose parents were both born in this area, was typed for AB0, MN, Kell, Rh, AK, EsD, 6-PGD, AcP and ABH secretor status. The village samples were aggregated into larger population units: Two districts and six subdistricts. Comparisons were performed with population samples of the plain and the coastal area of the same province (Lucca). Phenotype and genetic differentiations among and within subdivisions were studied using G2, R statistic, Nei's method, Harpending & Ward's method and analysis of genetic distance and similarity matrices. The various parameters consistently show significant heterogeneity among the subdivisions, both at district and at subdistrict level. As expected, the gene diversity between and within subdivisions varies according to their distinctive features of isolation.

Adult↗

The use of high resolution ultrasound in preoperative evaluation of cutaneous malignant melanoma thickness.

High resolution ultrasound (HRUS) was tested in 58 cutaneous malignant melanomas to check its validity in evaluating tumor thickness in vivo before surgery. The values obtained with this method were compared with histologic values (measured according to Breslow); a highly significant correlation was found (r = 0.895, p less than 0.001). The accuracy of HRUS in distinguishing between low- and high-risk cutaneous malignant melanoma was also found to be quite high. Our data therefore justify the use of such a technique in the preoperative staging procedure.

Adult↗

[Varicography in the preoperative assessment of primary varices. A study of 100 cases].

Primary varicose veins in the lower limb are usually well evaluated by the surgeon, who avails himself of tourniquet tests and Doppler US. However, the operation is often followed by failure or recurrence, or else it requires ugly skin dissection to eradicate an unforeseeably complicated varicose vein. We suggest that, whenever the surgeon considers the information obtained with tests and Doppler US inadequate for planning surgery, varicography be performed to get further morphological (and functional) data about the origin of varicose veins, which is of fundamental importance for an accurate surgical planning (cross-section stripping, ligation, sclerosis). Out of 100 varicographies consecutively carried out on patients in whom clinical examination was not conclusive, 62% allowed an already planned surgical approach to be changed. The figure rose to 100% in case of unusual varicose veins. Such a result allowed the needs of both the surgeon (selectivity, effectiveness, radicality) and the patient (narrow scars, absence of relapse) to be respected. Furthermore, it has been pointed out that some vein segments are often only pathological, and they are to be adequately treated, while others can be undamaged, and they are to be preserved for both their function and an eventual by-pass surgery. Varicography is rapidly performed, without complications, and is well accepted by the patient. It is a fundamental help in the preoperative study of primary varicose veins, especially in case of unusual ones, whenever the surgeon is in the slightest diagnostic--and therefore surgical--doubt.

Adult↗

Rh system in the Lucca province (Italy): temporal and spatial variations.

Phenotype and gene frequency distributions of the Rh system in the province of Lucca (Italy) are reported and discussed considering their diachronic and geographic variations. The data were collected in blood transfusion centers and refer to a total of 13,856 individuals. Analysis by chi 2- and R-matrix methods point to a highly significant genetic heterogeneity among the population subsamples through time. The results are discussed in relation to sample representativeness and to the main geographic and demographic characteristics of the province.

Gene Frequency↗

[The physiopathological basis for hemorrheological changes in diabetes].

Changes in blood viscosity and blood rheology have been reported in patients with diabetes mellitus. Rheology of blood flow in microcirculation is influenced by both general properties of blood (the so-called structural viscosity) and local conditions such as vessel size, blood flow rate, local pH and especially the presence of platelet aggregates. In patients affected by diabetes mellitus with or without vascular complications and in patients with impaired glucose tolerance, platelet hypercoagulability and hyperaggregability and a reduced prostacyclin production are present. Each one among these alterations can affect the rheology of blood flow. As a consequence of the blood clotting activation, plasma concentration of soluble fibrin complexes is increased and it can be responsible for an increase in plasma viscosity. Moreover, the increased tendency of platelets to aggregate observed in diabetes mellitus may result in the formation of aggregates which markedly increase viscosity in microcirculation. Finally, the reduced prostacyclin formation may be responsible for the reduction of erythrocyte deformability. Thus, changes in blood viscosity and rheological blood flow disturbances can be considered as a consequence of hemostatic system alterations.

Blood Coagulation↗

[Effect of prostacyclin on erythrocyte deformability and blood viscosity].

The aim of our research was to investigate the effect of prostacyclin (PGI2) on red blood cell deformability and blood viscosity. Ten non-smokers healthy subjects (aged from 25 to 40) were examined. Different PGI2 concentrations were added to the blood sample and then red blood cell deformability and blood viscosity were measured: 1.2 and 2.4 ng/ml PGI2 concentrations, corresponding to plasma PGI2-like activity in man, increased red blood cell deformability and reduced blood viscosity. The PGI2 capacity of affecting red blood cell deformability and blood viscosity seems to be of clinical interest in conditions where the PGI2 production is reduced, such as ischemic heart disease, peripheral artery disease and diabetes mellitus.

Adult↗