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Biomedical subjects

G Oliver

Publications and source records attributed to G Oliver.

At least 145 records · Page 8Linked to original sources

Comparative study of the efficiency of some additives in protecting lactic acid bacteria against freeze-drying.

Cultures of 14 lactic acid bacteria species were freeze-dried in 10 or 20% non-fat skim milk and in distilled water containing one of the following additives: bovine albumin, glycogen, dextran, polyethylene glycol (PEG) 1000, PEG 4000, PEG 6000, glycerol, beta-glycerophosphate, sodium glutamate, asparagine, or cysteine. Each of the potential protective agents tested exhibited marked variations in the protection afforded to different species, none of them was effective for the preservation of viability of thermophilic lactobacilli. However, glycerol provided effective protection for L. leichmannii ATCC 4797 (90% survival), while L. bulgaricus ATCC 11842 reached a viability of 78% with 0.04 M cysteine.

Asparagine↗

Protective effect of adonitol on lactic acid bacteria subjected to freeze-drying.

The protective effects of glycerol, adonitol, and four other related polyhydric alcohols on lactic acid bacteria subjected to freeze-drying were examined. The presence of adonitol in the suspending medium markedly protected the viabilities of the 12 stains tested. Dulcitol, mannitol, m-inositol, and sorbitol were found to provide little or no protection.

Cryoprotective Agents↗

Isolation and identification of anaerobic contaminants from a machine for producing processed cheese.

Some bacteria of the genus Clostridium can contaminate milk. These bacteria can cause "the late gas" or "late blowing" defect in the cheese if this is made with milk containing such contaminants. In this study, six samples from a processed cheese contaminated in a manufacturing machine were analysed. Out of 60 strains studied, 30 were classified as Clostridium tyrobutyricum, 20 as Clostridium butyricum, and 10 as Desulfotomaculum ruminis.

Cheese↗

Time-course of the effect of alpha-methyl-p-tyrosine on ACTH secretion.

250 mg/kg i.p. of the methyl ester of alpha-methyl-p-tyrosine (alpha-MpT) produced a rapid increase (by 1 h) in immunoreactive plasma ACTH, whereas the hypothalamic norepinephrine (NE) concentration and plasma corticosterone (B) changed significantly only 6 h after the administration of the inhibitor. These data suggest that the norepinephrinergic inhibitory tonus of CRF-ACTH secretion depends on the availability of a rapidly utilized pool of transmitter, affected in a very short time by alpha-MpT.

Adrenocorticotropic Hormone↗