Biomedical subjects

G N Gephardt

Publications and source records attributed to G N Gephardt.

44 records · Page 3Linked to original sources

PubMed1981

Malignant melanoma of the bronchus.

A case of malignant melanoma of the bronchus is presented. The tumor obstructed the left main stem bronchus. Secondary pneumonia was probably caused by Hemophilus influenzae.

Autopsy↗

PubMed1981

Comparison of alternative chromogens for renal immunohistochemistry.

Renal immunomicroscopy using enzyme labeled reagents has been shown to be a reliable method for the identification of immunoglobulins and complement in the routine evaluation of glomerular disease. However, the potential carcinogenicity of benzidine derivatives used in the procedure represents a major disadvantage of the technique. With a series of 55 renal biopsy specimens evaluated by light microscopy, immunofluorescence, and electron microscopy from patients with a variety of renal diseases, a study was done comparing aminoethylcarbazole and the Hanker-Yates reagent (p-phenylenediamine and pyrocatechol), chromogens chemically unrelated to benzidine. Aminoethylcarbazole was not suitable for renal immunomicroscopy, since in cases of antiglomerular basement membrane disease the color reaction product was finely granular at high magnification. Specimens immunostained with the Hanker-Yates reagent yielded permanent water insoluble reaction products and immunomicroscopic patterns identical to the results observed with immunofluorescence in all cases. To our knowledge, no carcinogenic properties have been identified for p-phenylenediamine or pyrocatechol. The Hanker-Yates reagent may be used routinely for renal enzyme immunomicroscopic studies with no currently identified carcinogenic hazard to laboratory personnel.

3,3'-Diaminobenzidine↗

PubMed1981

Cytoplasmic inclusions of Fabry's disease. Ultrastructural demonstration of their presence in urine sediment.

The diagnosis of Fabry's disease (angiokeratoma corporis diffusum universale) is usually confirmed by demonstrating typical cytoplasmic inclusions in a renal biopsy specimen, in conjunction with typical skin and ocular lesions and deficient alpha-galactosidase A activity in a male patient. Affected men are usually associated with carrier female family members. Electron microscopy of the urine sediment from two patients with Fabry's disease demonstrated typical laminated cytoplasmic inclusions within exfoliated epithelial cells. This method may be useful in the diagnosis of Fabry's disease and in screening of kin for hemizygotes and female carriers.

Cytoplasm↗

PubMed1981

Primary ultrastructural diagnosis of cryoglobulinemic glomerulonephritis.

A 58-year-old woman suffering from epistasis was found to be hypertensive. Hypocomplementemia associated with normal serum creatinine level, erythrocytosis, microscopic hematuria, proteinuria, and normal serum immunoglobulin levels and immunoelectrophoresis results led to a renal biopsy. Light microscopy disclosed mesangial hypercellularity and accumulation of eosinophilic material in capillary loops and in the mesangium. Immunomicroscopy disclosed coarsely granular to globular deposition of IgG, IgM, and C3 in similar distribution. Electron microscopy demonstrated electron-dense mesangial and subendothelial deposits consisting of interlacing fibrillar bundles associated with infiltration of acute inflammatory cells characteristic of cryoglobulinemic glomerulonephritis. After the biopsy, the presence of circulating IgG-IgM cryoglobulins was documented. Substructure of the in vitro cryoprecipitate and the immune complexes in the kidney was very similar. These observations illustrate the importance of continued routine ultrastructural assessment in evaluation of renal biopsy material.

Cryoglobulins↗

PubMed1980