Search PubMed⌕ Search

Biomedical subjects

G N Bisaga

Publications and source records attributed to G N Bisaga.

12 recordsLinked to original sources
PubMed2005

Clinical and quality of life responses to high-dose chemotherapy plus autologous stem cell transplantation in patients with multiple sclerosis: two case reports.

During the last several years high-dose chemotherapy (HDCT) with autologous stem cell transplantation (ASCT) has been established as a therapeutic option for multiple sclerosis (MS) patients. We report on the long-term effects of HDCT + ASCT in two female patients affected by secondary progressive and relapsing-remitting types of MS, respectively. As a result, disease stabilization was achieved in the first case and disease improvement in the second one. Both patients were off immunosuppressive or immunomodulating therapy throughout the post-transplant period. Notably, HDCT + ASCT resulted in an excellent quality of life (QoL) response in both cases. Our findings demonstrate that HDCT + ASCT could be considered as an effective treatment for MS patients. Moreover, QoL measurement seems to be an effective approach to assessment of treatment outcomes at long-term follow-up of patients with MS.

Adult↗
PubMed2002

[New approaches to antioxidant therapy in multiple sclerosis].

For study of antioxidant therapy efficiency in relapsing-remitting multiple sclerosis we investigated group 1 (18 patients) treated with alpha-lipoic acid and group 2 (14 patients) who received complex of antioxidants and neuroprotectors with various mechanisms of action (oc-lipoic acid, Nicotinamide, Acetylcysteine, Triovit Beta-carotine, Alpha-tocopheryl acetate, Ascorbic acid, Selenium, Pentoxifylline, Cerebrolysin, Amantadine hydrochloride) during 1 month, 2 times a year. The treatment resulted in significant reduction (2-3 times) of relapse frequency in multiple sclerosis patients (especially in group 2) and decrease of required corticosteroid courses. After antioxidant therapy the content of lipid peroxide products was significantly reduced (most expressed in group 2). The improved method of multicomponent antioxidant and neuroprotective therapy can be considered as pathogenic threatment in relapsing-remitting multiple sclerosis.

Adult↗
PubMed2001

[Proton magnetic-resonance spectroscopy in remitting and secondary-progressive multiple sclerosis].

Proton magnetic-resonance spectroscopy (PMRS) was used to measure the levels of inositol/myoinositol (Ins), choline, creatine/phosphocreatine (Cr), glutamine/glutamate (Glx/Glx1), N-acetylaspartate (NAA), gamma-aminobutyric acid (GABA) and lipids were measured in the foci of demyelinization in the brains of 59 patients with multiple sclerosis (MS). Magnetic-resonance imaging was performed during a single investigation. A control group comprised 20 healthy individuals. PMRS revealed significant alterations in the levels of metabolite in all the patients as compared with the controls: decreases in NAA by 23-52%, in Cr by 12-21%, in choline by 15-26%; increases in Ins by 51-63%; as well as the appearance of lipids (up to 100%). In MS, there were reduction in NAA/Cr, NAA/choline, and NAA/choline/Cr ratios by 12-53; 10-19; and 57-82%, respectively. As compared with the remitting MS, secondary-progressive MS showed decreases in the content of NAA by 23-25%, NAA/(choline + Cr) by 48-54% and increases in the levels of Ins and lipids by 50-76%. In remitting MS, there was a strong correlation between the NAA/Cr ratio and the volume brain lesion. It is concluded that PMRS evaluated the extent, pattern and activity of demyelinization (by the levels of Ins, NAA, Cr, lipids) and the intensity of cerebral atrophy (by NAA levels, NAA/Cr ratio). The findings testify that there are neurochemical differences between remitting and secondary-progressive MS.

Adolescent↗
PubMed2001

[Immunogenetic cytokine restriction in multiple sclerosis].

Sixty eight patients with verified multiple sclerosis (MS) (mean EDSS score 3.1 +/- 1.0) and 50 healthy donors have been investigated. Thirty five patients had relapsing-remitting, 25--secondary progressive, 8--primary progressive course. The remission was in 38, decompensation--in 20, relapse--in 10 patients. Lymphocyte subpopulations were investigated using monoclonal antibodies (Moscow) to the following antigens: CD3 (T-lymphocytes), CD4 (T-helpers), CD8 (T-supressors), CD20 (8-lymphocytes), CD25 (IL-2 receptor), CD16 (natural killers), CD95 (activated cells ready to apoptosis). Cytokines and tumor necrosis factor-alpha (TNF-alpha) levels were measured using ELISA test. HLA antigens were investigated by standard lymphocytotoxic test. In MS we found a fall of CD3, CD4, CD8, CD20 and CD16, but an increase of CD4/CD8, CD95, CD25. The CD95 level correlated with CD4, CD4/CD8 and CD16. In MS spontaneous IL-2, IL-6, IL-8 and TNF-alpha production was raised and stimulated IL-6 and IL-8 secretion was reduced. IL-4, IL-6, IL-8, TNF-alpha and IL-1 beta serum production in vivo was elevated. We found an increase of CD3, CD4, CD16, CD25, but a decrease of IL-1 (p < 0.01) spontaneous production and IL-6, IL-8, TNF-a stimulated secretion in DR2(+) MS patients, comparing to DR2(-) patients and controls. In DR2(-) patients as compared to DR2(+) patients and controls, all lymphocyte subpopulations levels, especially CD8 (p < 0.001) one, were decreased, but spontaneous IL-8 (p < 0.01) production was increased. The data obtained indicate lymphocyte apoptosis activation, targeting promoted lymphocyte destruction, and suggest T helper type-1 reaction prevalence in MS.

Adolescent↗
PubMed2000

[Multiple sclerosis in Northern-West region of Russia: results of HLA-typing].

Distribution of antigens of A, B, DR loci of HLA system in standard lymphocytotoxic test was studied in 59 patients with a significant diagnosis of multiple sclerosis (MS) and in 138 healthy donors. In the patients elevated frequency of the next antigens was found as compared with the controls: A10 (37%; chi 2 = 6.31; p < 0.05; relative risk--RR = 2.34), B7 (37%; chi 2 = 4.62; p < 0.05; RR = 2.05), B13 (29%; chi 2 = 10.86; p < 0.01; RR = 3.59), B35 (17%; chi 2 = 4.27; p < 0.05; RR = 2.61), DR2 (68%; chi 2 = 11.61; p < 0.001; RR = 2.99), as well as DR6 (5%; chi 2 = 3.95; p < 0.05; RR = 7.34) and also DRw52 (24%; chi 2 = 27.49; p < 0.001; RR = 21.16). The highest value of etiologic fraction was found for DR2 antigen. Analysis of intralocus and extralocus combinations of antigens in MS revealed that significantly elevated frequency had only one combination--B7DR2 (25.4%; chi 2 = 9.77; p < 0.01; RR = 3.58), relative risk was higher for this combination than for each individual antigen separately: B7 (RR = 2.05), DR2 (RR = 2.99). Significant negative associations with a possible protective effect of separate alleles were established in MS for antigens HLA A2 (34%; chi 2 = 5.55; p < 0.05; RR = 0.47), A11 (7%; chi 2 = 4.66; p < 0.05; RR = 0.31), A30 (0%; chi 2 = 4.50, p < 0.05; RR = 0.01), B18 (8%; chi 2 = 4.55; p < 0.05; RR = 0.35), DR5 (59%; chi 2 = 10.17; p < 0.01; RR = 0.36). The most significant was a decrease of the frequency of DR5 antigen (p < 0.01). Patients with the recurrent course had prevailed antigens A11, B21, B35 and decreased frequencies of antigens A9, B13, DR7. However, only the difference in the frequency of DR7 (16% in remitting and 57% in progredient course, chi 2 = 10.02; p < 0.001; RR = 0.14) was significant.

Adolescent↗
PubMed2000

Morphological changes in the skin during experimental allergic encephalomyelitis and multiple sclerosis: bases for new diagnostic test.

Skin specimen from the medial malleolar area of patients with multiple sclerosis and guinea pigs with experimental allergic encephalomyelitis were examined by light and electron microscopy. Demyelination, inflammation, and dystrophic changes in peripheral nerves and skin correlating with clinical manifestations were revealed in both sclerosis and encephalomyelitis. Maximum changes were revealed in nerve-associated cells (Merkel cells), Langerhans cells reflecting inflammation intensity, and fibroblasts responsible for nerve integrity and resistance against external stimuli.

Adult↗
PubMed1996

[Modern diagnostic methods in multiple sclerosis].

161 patients with multiple sclerosis were observed according to C. M. Poser's scale. The diagnosis was definite in 73.9% of cases, probable--in 14.9% and doubtful in 11.2% of patients. The diagnostic significance of magnetic resonance tomography (MRT) was estimated as well as of visual evoked potentials (VEPs), mid-latency auditory evoked potentials (MAEPs), viscocontrastoperimetry and pallesthesiometry (vibratory sensation). MRT revealed alterations in 80.3% of 71 cases (97.5% at definite diagnosis). The results of investigation of evoked potentials differed from normal values: VEPs in 100%, MAEPs in 72.6% of 26 patients observed. Visocontrastoperimetry revealed disorders in 75,2% of 113 cases, pallesthesiometry in toes--in 100%, in fingers--in 68.9% of 116 patients observed.

Adult↗
PubMed1992

[Role of specific brain peptide factors in the pathogenesis and compensation of neurologic disorders and their use in the treatment of nervous system diseases].

New concepts of the molecular mechanisms implicated in the pathogenesis and compensation of central motor disorders are presented. It has been shown experimentally that specific peptide, postural asymmetry, and inactivation factors, play the leading part in these processes. The detection of these specific factors in the cerebrospinal fluid (CSF) of patients and convalescents allowed one to work out and test clinically a new method of treatment (CSF therapy) of patients with central motor disorders of varying genesis. The results of the clinical observations of two patients' groups (with cerebrovascular brain lesions and multiple sclerosis) are provided.

Adult↗
PubMedDate not supplied

[New morphological data on multiple sclerosis].

The brain from 6 patients who died of multiple sclerosis was studied with MR tomography (MRT), macroscopy, light (CD 3, CD 20, LCA antigens) and electron microscopy (EM). Typical foci of demyelinization (plaques) were found in all cases. Alterations of brain arteries were found particularly in arteries of narrow lumen--with disturbance or even absence of elastin and muscle layer. Up to 30% of the vessels were surrounded by microcavities. Perivascular infiltrates consisting primarily of T lymphocytes were observed around the vessels (up to 70% of all vessels). This indicates the importance of vascular changes in the disease pathogenesis. Four variants of demyelinization (plaques) are distinguished depending on the degree of destruction of myelin, axons and glia. Two types of cells were found in the plaques with most pronounced changes: astrocytes and newly formed oligodendrocytes. Classification of plaques is suggested.

Adolescent↗