Biomedical subjects
Publications and source records attributed to G Mukherjee.
Studies were carried out on ferulic acid esterase production using a culture of Streptomyces S10. In optimized condition, enzyme yield was 2.0 mU/ml in MBS medium, containing 1.5% de-starched wheat bran at 30 degrees C and initial pH 6.5 under agitated submerged culture.
Three rotational bands in 74Kr were studied up to (in one case one transition short of) the maximum spin I(max) of their respective single-particle configurations. Their lifetimes have been determined using the Doppler-shift attenuation method. The deduced transition quadrupole moments reveal a modest decrease, but far from a complete loss of collectivity at the maximum spin I(max). This feature, together with the results of mean field calculations, indicates that the observed bands do not terminate at I = I(max).
The generic feature of traffic in a network of flowing electronic data packets is a phase transition from a stationary free-flow phase to a continuously growing congested nonstationary phase. In the most simple network of directed oriented square lattice we have been able to observe all crucial features of such flow systems having nontrivial critical behavior near the critical point of transition. The network here is in the shape of a square lattice and data packets are randomly posted with a rate rho at one side of the lattice. Each packet executes a directed diffusive motion toward the opposite boundary where it is delivered. Packets accumulated at a particular node form a queue and a maximum of m such packets randomly jump out of this node at every time step to its neighbors on a first-in-first-out basis. The phase transition occurs at rho(c) = m. The distribution of travel times through the system is found to have a log-normal behavior and the power spectrum of the load time series shows 1/f-like noise similar to the scenario of Internet traffic.
ArrayExpress is a public repository for microarray data that supports the MIAME (Minimum Information About a Microarray Experiment) requirements and stores well-annotated raw and normalized data. As of November 2004, ArrayExpress contains data from approximately 12,000 hybridizations covering 35 species. Data can be submitted online or directly from local databases or LIMS in a standard format, and password-protected access to prepublication data is provided for reviewers and authors. The data can be retrieved by accession number or queried by various parameters such as species, author and array platform. A facility to query experiments by gene and sample properties is provided for a growing subset of curated data that is loaded in to the ArrayExpress data warehouse. Data can be visualized and analysed using Expression Profiler, the integrated data analysis tool. ArrayExpress is available at http://www.ebi.ac.uk/arrayexpress.
A scale-free network is grown in the Euclidean space with a global directional bias. On a vertical plane, nodes are introduced at unit rate at randomly selected points and a node is allowed to be connected only to the subset of nodes which are below it using the attachment probability, pi(i)(t) approximately k(i)(t)l(alpha). Our numerical results indicate that the directed scale-free network for alpha=0 belongs to a different universality class compared to the isotropic scale-free network. For alpha - infinity. The link length distribution is calculated analytically for all values of alpha.
Disabled people in India frequently use the arm-crank propelled three-wheeled chair (ACWC) for outdoor transportation. Two models of these chairs are commercially available: one is powered by cranking using one arm (ACWC-1) and the other uses both arms (ACWC-2). The purpose of the study was to compare the efficiency of the two types of propulsion with respect to the standard physiological responses and, consequent upon the findings, to recommend the use of a suitable one. The energetics of locomotion of the users at their freely chosen speed (FCS) were measured and compared with changes in the physiological parameters in the within-subject groups. The study was conducted in outdoor settings to simulate the actual locomotive conditions encountered by the users in their practical life. 14 males, who had been regular and proficient users of both propulsion systems for more than last six years and who had a history of paraplegia (below the 10th thoracic vertebra, n=11) and poliomyelitis (n=3) participated in the study. The subjects were required to propel the test chair (a combination of both the propulsion systems built in a single model) on an oval track of 358 m circumference at their FCS for 5 min; ambulatory data were collected during last 3 min of exercise and averaged. The FCS (m/min), heart rates (bpm) and oxygen uptake (l/min) were measured. Physiological cost index (b/m) oxygen consumption (ml/kg/min), oxygen cost (ml/kg/m) and net locomotor energy cost (kcal/kg/km) of the two sets of observations were derived and compared using a t-test for the paired observations. The FCS was significantly higher and the physiological parameters were lower with the ACWC-2 than with ACWC-1, except for heart rate and oxygen consumption, where the difference was not significant . It is concluded that two-arm use is more efficient and less physically demanding than one-arm use in the arm-crank propulsion system and that the former is more suitable for efficient ambulation.
In an attempt to isolate chitinase producers from soil, a streptomycete strain was found potent using natural chitin as the substrate. Chitinolytic activity was tested directly on agar plates, also with crude enzyme. Chitinase assay showed that the isolate could produce 0.8 U/ml of the enzyme. The morphological, cultural, physiological and biochemical characters of the isolate P10 were studied, and identified as Streptomyces venezuelae P10.
We have measured the total energy of the alpha particles following the beta decay of 8B by implanting 8B into a planar silicon surface barrier detector. Calibration was performed using alpha particles following the beta decay of 20Na, similarly implanted. The alpha spectrum is used to infer the 8B neutrino spectrum which is an important input in the interpretation of experiments that detect energetic neutrinos from the Sun. The alpha spectrum reported here is in disagreement with the previous best measurement which used two detectors in coincidence.
Evidence is presented for multiphonon excitations based on a high-spin (25 Planck) intrinsic state in the deformed nucleus 182 Os. Angular momentum generation by this mode competes with collective rotation. The experimental data are compared with tilted-axis cranking calculations, supporting the vibrational interpretation. However, the lower experimental energies provide evidence that more complex interactions of states are playing a role.
S-phase kinase associated protein 2 (Skp2) is a member of the F-box family of substrate recognition subunits of SCF-ubiquitin ligase complexes and controls progression from G(1)-S-phase by targeting cell cycle regulators such as p21 and p27. Its locus is at 5p13, a region of frequent amplification in several cancers including carcinoma of the cervix (CaCx). Overexpression of Skp2 has been observed in many cancers of an advanced stage. We examine the expression of Skp2 in 42 invasive CaCx and its correlation with tumour differentiation state and p27 expression. Using immunohistochemistry we found increased nuclear expression of Skp2 in 55% of invasive CaCx cases analysed. It is significant that poorly differentiated tumours invariably exhibit high Skp2 expression (>40% positive nuclei), whereas well-differentiated tumours express Skp2 at a lower level (<20% positive nuclei). Skp2 expression in normal cervical epithelia is <10% (positive nuclei). Increased Skp2 protein levels did not correlate inversely with p27 expression. Our data suggest that Skp2 may contribute to the progression of CaCx, however, unlike non-human papillomavirus (HPV) containing tumours, p27 is unlikely to be the major target protein contributing to malignant progression. The high prevalence of HPV types in CaCx may circumvent the need for Skp2 to eliminate p27.
Structural properties of the Indian railway network is studied in the light of recent investigations of the scaling properties of different complex networks. Stations are considered as "nodes" and an arbitrary pair of stations is said to be connected by a "link" when at least one train stops at both stations. Rigorous analysis of the existing data shows that the Indian railway network displays small-world properties. We define and estimate several other quantities associated with this network.
A network is formed using the N sites of a one-dimensional lattice in the shape of a ring as nodes and each node with the initial degree k(in)=2. N links are then introduced to this network, each link starts from a distinct node, the other end being connected to any other node with degree k randomly selected with an attachment probability proportional to k(alpha). Tuning the control parameter alpha, we observe a transition where the average degree of the largest node changes its variation from N0 to N at a specific transition point of alpha(c). The network is scale free, i.e., the nodal degree distribution has a power law decay for alpha> or = alpha(c).
We report cytogenetic findings from fine-needle aspiration samples of two synovial sarcoma patients. The cases are of interest because (1) one case is of a rare site (submandibular region) of the head and neck, and (2) the other is a patient with synovial sarcoma of the toe showing additional cytogenetic abnormalities along with t(X;18). The literature of this tumor is reviewed.
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OBJECTIVE: To observe the effect of endurance training on novice users of an arm-propelled three-wheeled chair (APTWC), powered by asynchronous arm crank propulsion, in actual locomotive conditions. STUDY DESIGN: A continuous endurance training programme was administered by instructing subjects to propel themselves at free chosen speed (FCS) for 15 minutes, twice daily. Data were collected at two-week intervals consecutively and this was continued until the maximum beneficial effect was achieved. SETTING: The study was conducted in outdoor settings similar to those the users encountered in their everyday life. SUBJECTS: Twelve male paraplegics (below T10), age 30.5 +/- 8.59 years, weight 41.75 kg (+/- 5.76) recommended to use the APTWC as an ambulatory device. INTERVENTION: The propulsion speed (metres per minute), peak heart rate (beats per minute) and oxygen uptake (litres per minute) were measured in consecutive sessions at two-week intervals. Physiological cost index (beats m(-1)), oxygen consumption (ml kg(-1) min(-1)) and oxygen cost (ml kg(-1) m(-1)) were derived to assess fitness level. RESULTS: Significant changes were observed in the variables following endurance training for 12 weeks. The propulsion speed increased considerably and the cardiorespiratory parameters were found to be reduced. One-way analysis of variance was done for each parameter separately for seven sets of readings taken to assess the overall change over the study period of 12 weeks. The F-ratios were found to be significant (p < 0.01) in each case, except for body weight and oxygen uptake. The pairwise comparison of the biweekly results of the parameters which showed an overall significant change over the study period revealed that after the first two weeks there was considerable change (p < 0.01) for all the parameters. The trend was upward for speed and downward for the other cardiovascular stress parameters. These trends continued until the 10th week showing significant change, after which the values become stable as was revealed by thestatistical tests (p > 0.05). The percentage difference in the mean values from the initial stage (before training) to the 12th week ranged from 10.44% in the case of heart rate to 69.70% in the case of propulsion speed. CONCLUSION: Self-ambulation at free chosen speed regularly for 10-12 weeks provides efficiency and improves fitness status required for wheelchairs driven by an asynchronous arm crank propulsion technique and no special exercise programme is required.
PURPOSE: The Arm propelled three wheeled chair (APTWC) is in common use in the economically poor countries for outdoor ambulation, the propulsion force of which is exerted by arm cranking in asynchronous fashion. The purpose of the study was to assess the ambulatory performance of the users of the device using heart rate as a control index, at graded speeds. METHOD: Fifteen regular users of APTWC male with paraplegia and poliomyelitis were ambulated at seven different graded speeds in outdoor settings and the resting and ambulatory heart rate was measured during steady state and then physiological cost index and comfortable speed of propulsion was computed. RESULTS: The physiological strain, linear regression of the relationship between heart rate and speed with correlation coefficient of r = 0.86 (p < 0.001), polynomial regression of the relationship between physiological cost index (PCI) and speed with correlation coefficient of r = 0.83 (p < 0.001) and comfortable speed of propulsion (120 m/min) was reported. CONCLUSIONS: Heart rate can be used to evaluate the ambulatory performance of the device, quantifying energy economy for outcome evaluation and decision making for clinical recommendation.
Aberrant expression of FLT3 has been found in most cases of B-lineage ALL and AML, and subsets of T cell ALL, CML in blast crisis and CLL. In 20% of patients with AML the receptor has small internal tandem duplications of the juxtamembrane region which appear to contitutively activate the receptor. To investigate whether FLT3 activation could play a role in leukemia, we generated a constitutively activated FLT3 by fusing its cytoplasmic domain to the helix-loop-helix domain of TEL in analogy to the fusion that occurs with TEL-PDGFR in CMML. In vitro translation assays demonstrated oligomerization and intrinsic tyrosine kinase activity of the TEL-FLT3 chimeric receptor. Constitutively activated TEL-FLT3 conferred IL-3 independence and long-term proliferation to transfected Ba/F3 cells. Immunoblot analyses showed that JAK 2, STAT 3, STAT 5a, STAT 5b and CBL were tyrosine-phosphorylated in TEL-FLT3 expressing Ba/F3 cells in the absence of IL-3. These data suggest a possible role for the JAK/STAT pathway in FLT3 signaling. Transplantation of TEL-FLT3 expressing Ba/F3 cells into syngeneic mice caused mortality in all mice by 3 weeks after injection. Histopathologic analysis demonstrated a massive infiltration of mononuclear cells in the liver, spleen and bone marrow. The mimicking of naturally occurring TEL fusions provides an approach to assess aspects of the biology of activated FLT3, or other receptor-type tyrosine kinases (RTKs) in leukemic transformation.