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Biomedical subjects

G Moss

Publications and source records attributed to G Moss.

106 records · Page 6Linked to original sources
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Patents in the public interest.

The UK High Court has granted an injunction allowing Chiron Corporation a monopoly in selling hepatitis C virus test kits. The decision will be welcomed by the pharmaceutical and biotechnology industries.

Biotechnology↗
PubMedDate not supplied

Viable lymphocytes in frozen washed blood.

Samples of glycerolized, frozen, packed cells, were washed by each of three systems and then were cultrued for viable lymphocytes using the short-term culture method. Of the 39 samples, 17 (43.6%) showed definite evidence of growth of lymphocytes. Of the 17, 11(64.7%) were washed by Elutramatic, two (11.8%) by Haemonetics, and four (23.5%) by IBM. The 4 C prefreeze (two to five days) as well as the -80 C (21 To 40 days) storage times were the same for both positive and negative specimens. In all samples, clumps of distorted granulocytes with pyknotic nuclei were seen in addition to a number of well-preserved mononucleated white cells. In the 17 specimens which showed growth, incorporation of 3H-thymidine was seen only in the PHA-induced blast cells; typical mitotic figures were seen in some cultures. These observations demonstrate that lymphocytes remain viable in frozen blood stored at -80 C up to 40 days. The significant difference in removal of viable lymphocytes noted in specimens washed by different instruments requires further evaluation.

Blood Preservation↗
PubMedDate not supplied

Maintenance of GI function after bowel surgery and immediate enteral full nutrition. I. Doubling of canine colorectal anastomotic bursting pressure and intestinal wound mature collagen content.

Following either standardized stapled resection of an ileal blind loop terminus (17 beagles) or construction of a colorectal anastomosis (10 beagles), half the subjects immediately were fed an elemental diet (Vivonex HN). Gastrointestinal (GI) motility and absorption were maintained by exclusion of swallowed air. Controls received the same rate of feeding solution containing only the electrolyte components. Four days postoperatively, the fed beagle's colorectal anastomosis had over double the bursting pressure of the control, 303 +/- 46 mm Hg (p less than 0.001). At this time, the mature collagen content of the fed subjects' ileal wound was undiminished from that of normal ileum 2,223 +/- 336 versus 2,250 +/- 577, contrasting with the 45% decrease of this structural component (OHP) in the wounds of the unfed controls, 1,237 +/- 820 microgram/g of tissue (p less than 0.001). Similarly, the wounds of fed but not unfed subjects had a doubling (p less than 0.001) in concentration of collagen precursors and "new" collagen. The "catabolic" and "lag" phases, as noted in unfed experimental animals, appeared to be reflections of the relative starvation that accompanied the intestinal wounding. With maintenance of GI function and immediate full enteral nutrition, "accelerated healing" was noted relative to the previously considered "norm".

Animals↗
PubMedDate not supplied

Efficient gastroduodenal decompression with simultaneous full enteral nutrition: a new gastrostomy catheter technique.

A gastrostomy tube was designed that provides efficient supplemental aspiration of the proximal duodenum to remove gas that escapes gastric removal, utilizing an extension of the gastric suction channel. A separate lumen delivers elemental diet 7.5 cm more distally, for safe postoperative feeding. On clinical testing, patients consistently achieved immediately postoperative enteral absorption of 3000 kcal/day and positive protein balance. Supplemental insulin was required for optimum utilization, maintaining plasma glucose within the physiologic range. Oral barium motility study during the second 24 hr demonstrated prompt gastric emptying and spontaneous anastomotic passage. We noted decreased clogging with mucus, attributable to "self-cleansing" by the aspirated duodenal juices. This was returned with the feedings to prevent depletion. There were no complications attributable to this regimen. Patients were discharged uneventfully as early as 48 hr following colectomy.

Catheterization↗