Biomedical subjects

G Mogi

Publications and source records attributed to G Mogi.

154 records · Page 9Linked to original sources

PubMedDate not supplied

Immunofluorescent study on middle ear mucosa.

To clarify the developmental course of the immunological defense system in the middle ear, immunoglobulin-forming cells of different classes and secretory proteins, such as secretory component (SC) and lactoferrin (Lf), were investigated in the middle ear mucosa of 20 developing and 5 normal adult guinea pigs by use of direct immunofluorescence technique. Changes in the middle ear mucosa were also observed after antigenic challenges directly to the tympanic cavity of 6 developing and 39 adult guinea pigs. IgA- and IgM-forming cells began to appear in the tubal mucosa at the seventh postnatal day, while it was scarcely possible to find IgG1- and IgG2-forming cells in developing guinea pigs. Immunoglobulin-forming cells of all classes increased in the middle ear mucosa after the antigenic stimuli. The injection of antigens to the tympanic cavity of developing animals induced the most striking accumulation of immunoglobulin-forming cells in the middle ear mucosa. Results of this study showed that local synthesis of IgA, as well as other classes, is latent in the middle ear, that the middle ear of immature animals is vulnerable to antigenic stimuli, and that the middle ear of developing animals possesses potential immune responsiveness.

Animals↗

PubMedDate not supplied

Secretory IgA and serum type IgA in nasal secretion and antibody activity against the M protein.

We studied IgA immunoglobulins in nasal secretions in order to clarify mucosal immunity of the nasal cavity and paranasal sinuses during chronic nasal infection. Secretory IgA and serum type IgA of 165 samples of nasal secretions were analyzed quantitatively by use of electroimmunodiffusion techniques, and the specific antibody activity of secretory IgA against the M protein of Streptococcus pyogenes was investigated by use of enzyme-linked immunosorbent assay. Results show that although the secretory IgA content in nasal secretions was elevated in chronic sinusitis, its specific antibody activity against the M protein was lower than that in normal subjects. This evidence suggests that nonspecific secretory IgA antibodies are predominantly produced in chronic sinusitis, and that mucosal immunity preventing the adherence of bacteria is impaired in the diseased mucosa.

Adolescent↗

PubMedDate not supplied

Effects of anti-allergic drugs on substance P (SP) and vasoactive intestinal peptide (VIP) in nasal secretions.

To clarify the effects of anti-allergic drugs on substance P (SP) and vasoactive intestinal peptide (VIP) levels in nasal secretions, we employed competitive enzyme-linked immunoassays to measure concentrations of those neuropeptides in nasal secretions from 40 patients with house dust nasal allergy before and after administration of azelastine and oxatomide. One mg of azelastine and 30 mg of oxatomide were administrated twice a day for 4 weeks. Mean values of SP concentrations and ratios of SP to total protein of the nasal allergy group were significantly higher than those of the control group (p < 0.002). The VIP/total protein ratio of the allergy group was also significantly higher than that of the control group, although the VIP concentration alone was not. Mean levels of SP and VIP from patients with severe symptoms were significantly higher than those of the control group (p < 0.05), although those values were not significantly different between patients with moderate symptoms and control subjects. Azelastine and oxatomide effectively reduced SP levels in nasal secretions (p < 0.005), but they did not significantly decrease VIP levels. The reduction of SP levels was significant in patients with excellent responses to those drugs (p < 0.005), but not in patients with poor responses. These findings suggest that SP and VIP levels in nasal secretions may reflect the clinical state of nasal allergy and be one of the better parameters available for evaluating the clinical efficacy of anti-allergic drugs against nasal allergy.

Adolescent↗

PubMedDate not supplied

Radioimmunoassay of IgE in middle ear effusions.

Fifty-six sample pairs of middle ear effusions and sera obtained from patients with otitis media with effusion were investigated for The IgE antibody activity against mites, using radioallergosorbent test (RAST) which was recently developed as an in vitro allergy test. The IgE concentrations were also determined by radioimmunosorbent test (RIST). Results showed that the IgE antibody activity against this allergen was found in 5 middle ear effusions and 3 sera. Four of 5 patients with the positive RAST test had nasal allergy or signs suggesting allergy. Theree were two middle ear effusions which seemed to be an allergic fluid. However, findings in the present study do not suggest that middle ear effusions result from the direct allergic reaction induced by mites in the mucous membrane lining the middle ear cavity.

Acute Disease↗

PubMedDate not supplied

IgA immunocytes in tonsils.

Dimeric IgA-forming cells were studied by a secretory component (SC) affinity test on 20 palatine and 7 pharyngeal tonils from children. This study also included an investigation on the immunoflurescent localization of IgA immunocytes and IgA and SC deposits. The results showed that IgA immunocytes capable of binding SC in tissue sections are present in both palatine and pharyngeal tonsils. However, the number of cells positive for the SC affinity test was significantly lower than that of IgA immunocytes not binding SC. IgA immunocytes were located mainly in the subepithelial area, medullary portion, and occasionally the intraepithelial layer. SC determinants were detected only in some epithelial cells of the pharyngeal tonsils. The findings of the present study suggest that the pharyngeal tonsils share in the local immunological mucosal resistance regulated by secretory IgA, although its activity might be limited.

Adolescent↗

PubMedDate not supplied

Laryngeal secretions. An immunochemical and immunohistological study.

This study was designed to investigate properties of laryngeal secretion and secretory activity of IgA in the larynx. Laryngeal secretions were collected by adsorption method on filter paper during laryngomicrosurgery from 20 patients having an inflammatory lesion in the larynx. Contents of IgG, IgA, IgE, secretory component (SC), and lactoferrin in the laryngeal secretions were determined and compared with results of those in nasal secretions, tracheobronchial washings, and serum samples obtained from the same subjects. The laryngeal mucosae of 8 laryngectomized materials for cancer lesion were subjected to immunofluorescence studies including the cytoplasmic SC affinity test. Results of this study indicate that laryngeal secretions are characterized by exocrine secretion, resembling nasal and tracheobronchial secretions in the electrophoretic pattern and immunoglobulins content. The immunofluorescence studies and SC affinity test found that the larynx possesses secretory activity of IgA, particularly in the ventricle and subglottis.

Adult↗

PubMedDate not supplied

The efficacy of sulphate quinine compared to fansidar in treating falciparum malaria in children 6 months-7 years old.

From January 1989 until February 1990, 65 children suffering from falciparum malaria without complication at the Department of Child Health Medical School Sam Ratulangi University/Gunung Wenang Hospital Manado were randomly allocated into two groups. The first group was treated with sulphate quinine based on the dose of 30 mg/kg bw/day in three divided doses and the other one was treated with single dose fansidar (sulphadoxine-pyrimethamine) based on the dose of sulphadoxine 20-30 mg/kg bw/day. The group treated with quinine showed much shorter duration of parasitemia compared with the group treated with fansidar. Fever subsided more rapidly in children treated with quinine compared with those treated with fansidar.

Antimalarials↗

PubMedDate not supplied

Experimental otitis media in chinchillas. II. Comparison of the middle ear immune responses to S pneumoniae types 3 and 23.

The present study shows that two different serotypes of Streptococus pneumoniae (type 3 and 23) when inoculated through the bulla elicit distinctly different immune responses in chinchillas. The passive hemagglutination assay shows that type 3 is a better immunogen than type 23 and that type 3 primarily elicits immune responses in the middle ear. When class-specific antibody activity was measured with the red cell linked antigen-antibody reaction test, antibody activities of serum IgG to type 3 slightly declined and of effusion IgM and IgA to type 3 increased following type 3 infection, whereas following type 23 infection antibody activities of serum IgG to type 23 increased and no antibody activities of IgA and IgM to type 23 were present in the effusions.

Animals↗

PubMedDate not supplied

Microcrypt extensions of tonsillar crypts.

The crypts of human palatine tonsils were examined electron microscopically. The cryptal epithelia contained three types of micropores (termed microcrypt). Type I microcrypts opened between intercellular spaced of the epithelial cells. Free cells were observed in the micropores. Type II microcrypts opened near the center of a cryptal epithelial cell. Type III microcrypts appeared after "specialized epithelial cells" degenerated and fell off. Many mature plasma cells are attached to the walls of the sinusoidal vessels in the lower boundary zone of the epithelium and subepithelial layer. Lymphocytes were observed migrating from the postcapillary venules of the subepithelial zone. The findings indicate that the microcrypts are probably orifices for tunnel-like passages of free cells and that they are located in the walls of the tonsillar crypts. The lymphocytes in the passages perhaps come from lymphoid tissues in the subepithelial layer and postcapillary venules, and the antibodies produced in the cryptal epithelia of the palatine tonsils may contribute to humoral immunity.

Adolescent↗

PubMedDate not supplied

IgE in respiratory tract allergies.

The IgE concentration was determined in the sera and nasal secretions of 166 patients with nasal allergy, 40 patients with bronchial asthma, and 50 control subjects, using Phadebas IgE kits. The IgE level was significantly elevated in both the sera and nasal secretions of allergic patients. The sera and nasal secretions of 142 patients, who were positive in HD or mites skin test, were subjected to a radioallergosorbent test (RAST) for estimating the specific IgE antibody activity to mites. The gradings of the specific antibody activity were significantly correlated with the strengths of the HD skin tests. The results of this study proved the usefulness of the determination of IgE and specific IgE antibodies in the diagnosis and study of the pathogenesis of respiratory tract allergies. The IgE in nasal secretions was much more closely related to pathogenesis of respiratory allergies than was IgE in sera. However, unless more concentrated nasal secretions are assayed, the diagnostic value is limited.

Adolescent↗