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Biomedical subjects

G Micali

Publications and source records attributed to G Micali.

At least 91 records · Page 5Linked to original sources
PubMed1983

Changes in rapid transport of phospholipids in the rat sciatic nerve during axonal regeneration.

Axonal transport of phospholipids in normal and regenerating sciatic nerve of the rat was studied. At various intervals after axotomy of the right sciatic nerve in the midthigh region and subsequent perineurial sutures of the transected fascicles, a mixture of 60 mu Ci [Me-14C]choline and 15 muCi [2-3H]glycerol in the region of the spinal motor neurons of the L5 and L6 segments was injected bilaterally. The amount of radioactive lipid (and in certain cases its distribution in various lipid classes) along the nerve was determined as a function of time. Three days after fascicular suture and 6 h after spinal cord injection of precursors, there was an accumulation of labeled phospholipids and sphingolipids in the transected sciatic nerve in the region immediately proximal to the site of suture. Nine days after, there was a marked increase in the accumulation of radioactivity in the distal segments of the injured nerve, which increased up to 14 days after cutting and disappeared as regeneration proceeded (21-45 days). In all segments of both normal and regenerating nerve fibers, as well as in L5 and L6 spinal cord segments, only phosphatidylcholine and sphingomyelin were labeled with [14C]choline. These results suggest that the regeneration process in a distal segment of a peripheral neuron, following cutting and fascicular repairing by surgical sutures, is sustained in the first 3 weeks by changes in the amount of phospholipids rapidly transported along the axon towards the site of nerve fiber outgrowth.

Animals↗
PubMed1975

[Experimental and clinical studies on the use of a new suture material: polyglycolic acid].

Experimental and clinical investigation of polyglycolic acid, a new synthetic suture material, is reported. Experimental work was done on single-layer intestinal sutures, while clinical employment of the material was extended to various general surgery operations. There were few signs of inflammation at the site of implant, indicating that the new material has a greater degree of inertness than chronic catgut, restulting in optimal tolerance on the part of the body.

Animals↗