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Biomedical subjects

G Meulemans

Publications and source records attributed to G Meulemans.

At least 37 records · Page 2Linked to original sources
PubMed1987

Pathogenicity of antigenic variants of Newcastle disease virus Italian strain selected with monoclonal antibodies.

Antigenic variants of the Italian strain of NDV were selected using monoclonal antibodies directed against the HN and F proteins of Italian virus. Antigenic mapping of the HN and F proteins using variant viruses in cross neutralization tests revealed the presence of at least two different epitopes on HN and four epitopes on F protein. Immunoselected variant viruses were demonstrated to have different intravenous pathogenicity index than the parental Italian virus.

Antibodies, Monoclonal↗
PubMed1986

Antigenic and biological characterization of avian paramyxovirus type 1 isolates from pigeons.

21 A/PMV-1 viruses were isolated from pigeons and characterized using polyclonal and monoclonal antisera in hemagglutination inhibition, seroneutralization and immunoprecipitation studies. Polyclonal and monoclonal antibodies directed against the HN and F proteins of Italien virus reacted with all pigeon isolates showing a close relationship between chicken velogenic and pigeon viruses. Differences in the M.W. of F0, P and M proteins were however observed between pigeon and chicken Italien virus. Marked differences in virulence were recorded among pigeon isolates; these were reflected by great variation in the IVPI of the different strains.

Animals↗
PubMed1986

Monoclonal antibodies to hemagglutinin-neuraminidase and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity.

Eighteen hybridoma lines obtained by immunization of mice with Newcastle disease virus (NDV) lentogenic strain La Sota or velogenic strain Italien produced hemagglutinating monoclonal antibodies. The 18 monoclones were divided into four groups according to their reactivity toward native hemagglutinin neuraminidase protein (HN), nonglycosylated HN precursor, and heat-denatured HN blotted on nitrocellulose membranes. Only group II reagents were reactive toward their targets in all conditions tested. They were considered sequence-specific antibodies. Group I antibodies did not require glycosylation but lacked reactivity towards the denatured glycosylated antigen. Monoclonal antibodies from group III recognized only the native HN. Group IV was made up of a single monoclone that lacked reactivity with NDV Italien but recognized the La Sota strain in hemagglutination inhibition and enzyme-linked immunosorbent assays. Five hybridoma lines produced monoclonal antibodies which neutralized viral infectivity but failed to inhibit hemagglutination. One monoclonal antibody obtained after immunization of mice with NDV La Sota showed a low neutralization index versus NDV Italien. Four monoclonal antibodies derived from mice immunized with NDV Italien showed higher neutralization indices towards this strain. Neither the denatured F protein nor its nonglycosylated precursor was reacted against by the five monoclonal antibodies.

Animals↗
PubMed1985

[Cervical tumors].

Explore the source record for details and available documents.

Head and Neck Neoplasms↗
PubMed1983

Isolation of orthoreoviruses from psittacine birds.

Orthoreoviridae were regularly isolated from imported psittacine birds in the absence of other pathogens or in combination with salmonella. These viruses grew in embryonated eggs, in chicken embryo fibroblasts and in hepatic cell cultures. The viral isolates were classified as orthoreoviridae on the basis of their morphological and physico-chemical properties.

Animals↗
PubMed1981

[Myeloma and hypereosinophilic leukemoid reaction (author's transl)].

In a 56-year-old man with a myeloma marked eosinophilia was discovered. The diagnosis of myeloma was made on the discovery of plasmocyte infiltration in the mandible. Despite surgery and chemotherapy, general bone infiltration by plasma cells occurred over 15 months. The leukocyte count rose to 226,000/mm3 with 162,720 mature eosinophils. Pathogenic problems suggested by such an unusual association (myeloma-eosinophilic leukemoid reaction) are considered.

Eosinophilia↗
PubMed1981

[Microscopic and ultramicroscopic lesions from experimental mycoplasma infection in respiratory tract of chickens. Possible difference between pathogenic and non pathogenic strains (author's transl)].

For three weeks after experimental infection of trachea and scroll bone with Mycoplasma gallisepticum no lesions were detected in scroll bone, but only an abundant mucous secretion. Mycoplasmas were present and adhered to cilia of epithelial cells. In trachea an almost complete destruction of cilia was observed allowing swollen epithelial cells to appear with mycoplasmas adhering to villi. Strain W31 serotype C caused no lesions but cells and cilia were covered with a thick layer of mucous.

Animals↗