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Biomedical subjects

G Messeri

Publications and source records attributed to G Messeri.

At least 73 records · Page 4Linked to original sources
PubMed1980

Lipid content of amniotic fluid cells.

The lipid content of fetal cells was determined in 45 samples of human amniotic fluid. Free and total cholesterol were estimated using a gas chromatographic method, and glycerides were evaluated through the enzymatic assay of their glycerol content. The number of orange cells was estimated after staining with Nile Blue sulphate. The chemically measured lipid content appeared closely related to the number of orange cells. Total cholesterol and glycerides showed a sharp increase after the 37th week of pregnancy. These tests seem to assess fetal maturity successfully, providing a further useful aid in the management of high-risk pregnancies. The chemical determination of cell-associated lipids showed good accuracy and reliability and, when compared with the histochemical method, allowed a better evaluation of progressive lipid accumulation within the amniotic fluid cells.

Amniotic Fluid↗
PubMed1979

[Oestrogen receptors determination in human breast cancer (author's transl)].

The AA. refer about the assessment of a charcoal-dextran method for the determination of oestrogens receptors in human breast cancer. Corrections for low affinity binding components are performed measuring bound 3H-17 beta estradiol in the absence and presence of non-radioactive 17 beta estradiol or diethylstilbestrol; the method has been previously studied on samples from pregnant rabbit uterus and then applied on human mammary tumors. Results achieved with the routine-utilisation of the present method are reported.

Breast Neoplasms↗
PubMed1974

[Gas-chromatographic method for determination of plasma lecithin-cholesterol acyl transferase (LCAT) activity].

A method is described for the determination of plasma lecythin-cholesterol acyl-transferase activity (LCAT), based on the measuring ot the free cholesterol concentration decrement in incubated plasma, with the aid of gas-liquid chromatography. Precision evaluation showed a sufficient reliability of the method, which is considered to be proposable for practical puopose. After verifying normal values in a group of healthy adults, plasma LCAT reduction in liver diseases, specially in the chronic hepatic disease, was confirmed.

Acyltransferases↗
PubMedDate not supplied

Measurement of urinary somatomedin C by a chemiluminescent method.

We present a method for the measurement of the total Somatomedin C (SmC) content in human early morning urine samples after dialysis, extraction, and concentration. We modified a chemiluminescence immunoassay, previously developed for SmC determination in serum, for analysis of SmC in urine. Appropriate sensitivity was obtained by the preparation of a new chemiluminescent tracer (AEEI-COOH-SmC) and the optimization of a competitive non-equilibrium immunoassay system which had a detection limit of 0.24 fmol SmC per tube.

Carrier Proteins↗
PubMedDate not supplied

[Clinicacy].

Explore the source record for details and available documents.

Estriol↗
PubMedDate not supplied

Estrone 3-glucuronide chemiluminescence immunoassay (LIA) and 17beta estradiol radioimmunoassay (RIA) in the monitoring of superovulation for in vitro fertilization (IVF): correlation with follicular parameters and oocyte maturity.

Many works in the literature of the last years had reported that urinary approach to superovulation study is a suitable method to evaluate ovarian response to pharmacological stimulation. Before applying urinary determination of hormonal levels with a chemiluminescence immuno assay (LIA) method in early morning urine (EMU) samples, we had studied the correlation of RIA-LIA procedures with reference to follicular volumes at hCG day and to recovered oocyte maturity; in fact follicular growth and oocyte morphological features are the main parameters to evaluate a successful induced cycle. In our department the IVF cycles are daily monitored with RIA seric E2 and LIA E1-3G determination, besides ultrasound examination of follicular growth. We have studied E2 and E1-3G levels on the hCG administration day and their correlation with follicular areas and volumes; moreover, we have evaluated hormonal values on oocyte pick-up day with reference to recovered oocyte number and maturity. We have assumed as good timing for oocyte pick-up when more than 50% of recovered oocytes were of good quality (maturity score 4). We have observed that the highest pre ovulatory E1-3G value is consistent with the best timing for oocyte pick-up; it's possible to obtain a conversion coefficient follicular volumes and urinary E1-3G excretion. We have not found significant differences between plasmatic and urinary estrogenic parameters. It is important to remember the advantages connected by a not isotopic and not invasive method. The absence of discomfort for the patients may be a decisive factor to choose the monitoring method and LIA procedure may represent a valid alternative to RIA.

Adult↗
PubMedDate not supplied

Interference of exogenous HCG on pregnancy tests.

New urinary tests of pregnancy have recently been developed that are exceptionally sensitive to HCG. Our study was undertaken to explore the possibility that exogenous HCG, administered to induce ovulation, may interfere with these low-threshold tests of pregnancy. Six healthy volunteers participated in our study. Each woman received three sequential 5,000-U doses of HCG by im route on days 3, 5, and 7 after ovulation. Blood and urine for HCG assay were taken daily from each subject. Urine specimens were subjected to a traditional high-threshold pregnancy test (sensitivity limit = 2000 U/L) and to a low-threshold pregnancy test (sensitivity limit = 50 U/L); HCG in plasma and urine was determined by ELISA assay. Plasma HCG levels increased gradually under HCG dosing and attained a peak on day 8 (average = 73.7 U/L; range 24-124 U/L). As regards urinary HCG levels, the average value on days 8, 9, and 10 was above the 50-U/L detection limit of the low-threshold test, which was in fact positive in the majority of patients. Also on days 11 and 12, however, urinary HCG levels were above this limit in a certain proportion of subjects (day 11, 2/6; day 12, 1/6); accordingly the low-threshold pregnancy test was positive with a frequency of 2/6 on day 11 and of 1/6 on day 12. By contrast, the traditional high-threshold pregnancy test was negative in urine samples of all subjects. Our study indicates that the increased sensitivity of modern low-threshold pregnancy tests may cause false positive results in those cases where exogenous HCG has been administered during the luteinic phase.

Adult↗