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Biomedical subjects

G Matthes

Publications and source records attributed to G Matthes.

83 records · Page 5Linked to original sources
PubMed1979

[Pretreatment of donors with sodium selenite-a method for the conditioning of tissues for cryoconservation].

Investigations about the protective effect of the pretreatment of albino rats with different concentrations of sodium selenite in sodium chloride solution were carried out. Albino rats, serving as a control group, had only a pretreatment with sodium chloride solution. The following results were obtained: 1. An intraperitoneal application of 2 microgram sodium selenite per kg body weight over three days shows a significant increase of contractility of frozen and afterwards re-thawed fragments of heart-muscle after storage -196 degrees C. 2. The increase or decrease of the sodium selenite dose by the factor ten had no effect on the preservation of the contractility of fragments of the heart-muscle after storage -196 degrees C in comparison to the control group. 3. A differentiated observation of the injuries under frozen preservation permitted the conclusion that the pretreatment with sodium selenite for the most parts will be protective in the phase below 0 degrees.

Animals↗
PubMed1979

Some aspects for the further improvement in cell cryopreservation.

According to theoretical considerations and to results of own experimental investigations in heart muscle fragments of adult rats the authors are of opinion that more attention should be attributed to the cryoprotective medium as a whole, i.e. not only to the proper cryoprotectant, but to the other components of the cryoprotective medium as well. The suppose that the still unsatisfactory results of cell preservation, including blood and bone marrow cells, can be essentially improved by optimizing the composition of the basal medium.

Animals↗
PubMed1979

[Protective effect of selenium compounds in the freeze preservation of erythrocytes].

The influence of sodium selenite, selenomethionine and sodium selenite with tocopherols in combination on the survival of cryopreserved erythrocytes was investigated. Percent hemolysis is marked decreased after a three-hour incubation of the whole blood with addition of selenomethionine as well as sodium selenite with tocopherole in combination before cryopreservation. The protective effect is attributed to the antioxydative and membrane stabilizing effectiveness of selenium and tocopherol.

Blood Preservation↗
PubMed1978

Successful cryopreservation of auricle fragments from rat hearts at -196 degrees C.

The influence of electrolyte composition and glucose concentration of a cryoprotective medium on the survival of auricle fragments from adult rat hearts after storage at -196 degrees C was investigated. Using a K+-, Mg++-, Ca++-rich solution with increased glucose concentration, a high rate of surviving fragments was found after cryopreservation.

Animals↗