Search PubMed⌕ Search

Biomedical subjects

G Mancini

Publications and source records attributed to G Mancini.

At least 73 records · Page 4Linked to original sources
PubMed1985

Quantitative determination and separation of analogues of aminoglycoside antibiotics by high-performance liquid chromatography.

Commercial bulk products and pharmaceutical drug formulations of aminoglycoside antibiotics obtained by fermentation (kanamycin, gentamicin, sisomicin and tobramycin) or by synthesis (amikacin) were analysed with high-performance liquid chromatography on a C8 reversed-phase column. The method is based on a pre-column derivatization of the aminoglycosides with a 2,4,6-trinitrobenzenesulphonic acid reagent and UV detection (350 nm). The quantitative determination was carried out vs. an external standard; both peak heights and areas were used. A gentamicin mixture was separated into five or four components, depending on the column used. Amikacin was separated from its possible regioisomers and kanamycin A was easily separated from its minor components B and C.

Amikacin↗
PubMed1985

Antigenic and biochemical analysis of influenza "A" H3N2 viruses isolated from pigs.

Four influenza A-H3N2 viruses isolated in pigs from different herds in Central Italy in the period 1981/82 have been antigenically and biochemically analysed. Three of them A/Sw/Italy/2/81, A/Sw/Italy/7/81, A/Sw/Italy/8/82 were found to be serologically related to A/Bangkok/1/79 (H3N2). These three viruses were shown to have an identical electrophoretic pattern, as regards virus induced polypeptides and were clearly distinguishable from the virus A/Sw/Italy/6/81 which was antigenically related to A/England/42/72 (H3N2) and A/Sw/Taiwan/7310/70 as shown by specific monoclonal and polyclonal antisera. The observed biochemical variations underline the importance of the changes occurring by genetic reassortments or mutations in human influenza viruses, during their maintenance in pigs.

Animals↗
PubMed1985

Comparative electrophoretic study of polypeptides of influenza A/H3N2 viruses isolated in circumscribed geographical areas.

Two distinct groups of influenza A/H3N2 viruses, closely related to A/Bangkok/1/79 and to A/Belgium/2/81, have been chosen from viruses isolated in Italy during 1981 to 1983 with the aim of analysing the biochemical composition of their polypeptides. The strains of each group have shown differences in electrophoretic migration rates in one or more proteins in comparison to the prototype viruses. Polypeptide mobility variations among isolates from circumscribed geographical areas and from single outbreaks have also been observed. In particular, there was a high degree of variability in the NS1 protein. The detection of biochemical differences among identical antigenic variants, probably the result of point mutations in polypeptide sequences or of genetic reassortment among different co-circulating human viruses, is a further expression of the peculiar ability of the influenza A virus to exhibit variation in internal proteins during its circulation.

Disease Outbreaks↗
PubMed1984

Detection of antibodies to strain-specific and cross-reactive antigenic determinants on the haemagglutinin of influenza viruses A/Texas/1/77 and A/Bangkok/1/79 in human sera. Brief report.

The antibody response to antigenic determinants on the haemagglutinin molecule of A/Texas/77 and A/Bangkok/79 strains was analysed by SRH of virus-absorbed sera, in human sera collected from persons of different ages following natural infection or immunization. The results have shown, as expected, low and high frequencies of strain-specific antibodies in sera of adults and young children respectively. However, in the latter group antibodies to cross-reactive antigens were also detected.

Adolescent↗
PubMed1984

Detection of antibodies of the IgG and IgM classes to herpes simplex virus type 1 in cerebrospinal fluid and serum from four patients with herpes encephalitis.

Antibodies of IgG and IgM classes to Herpes simplex virus type 1 were detected, using the ELISA technique, in the cerebro-spinal fluid (CSF) and in the serum of four patients with encephalitis. All CSF and sera were positive for IgG antibodies; two CSF samples, which were collected quite early, were also positive for antibodies of IgM classes.

Antibodies, Viral↗
PubMed1984

Children's memory for phonemically confusable and nonconfusable letters: changes with age and reading ability.

This experiment focused on differences that occur with age and reading skill in the use of phonemic codes in short-term retention tasks where stimuli were presented visually. In 1 condition, individuals recalled 6-letter strings composed of rhyming and nonrhyming letters after a 15-sec delay under conditions that permitted phonemic coding and rehearsal. 2 other conditions were designed to suppress (a) rehearsal of certain types of phonemic codes in the delay interval and (b) both phonemic coding at stimulus presentation and rehearsal. Subjects were grade 2 average readers matched in reading skill with grade 4 disabled readers, grade 4 average matched with grade 6 disabled, and grade 6 average matched with grade 4 superior readers. Average readers showed a decrease in errors with age in all 3 conditions, although performance was always better in the nonsuppression condition. In the latter, memory for nonrhyming letters was always better than memory for rhyming letters. Differential use of phonemic codes by good and poor readers in grades 4 and 6 was less than that found in previous research with younger children. Finally, by comparing the performances of grade 2 average with grade 4 disabled readers and grade 4 average with grade 6 disabled readers, the developmental lag hypothesis of reading disability was examined. Problems with testing the hypothesis are discussed.

Child↗
PubMed1984

Hemopoietic development in human embryos.

We report studies on hemopoietic progenitors and precursors in human embryos and fetuses at 4 to 5 through to 9 to 10 weeks from conception, as well as the corresponding in vivo Hb synthesis pattern. Particular attention is paid to the morphology of 5- to 8-week liver cells, as evaluated by both conventional and electron microscopy. On the basis of these observations, a unifying monoclonal model is proposed for human embryonic hemopoiesis.

Cells, Cultured↗
PubMed1983

Comparison of haemagglutination-inhibition and single radial haemolysis techniques for detecting antibodies to influenza A and B viruses.

The sensitivities of haemagglutination-inhibition (HI) and single radial haemolysis (SRH) techniques in detecting antibodies against influenza A/Bangkok/1/79, A/Brazil/11/78, B/Singapore/222/79, B/Hong Kong/5/72 strains, in human sera were compared. For antibodies to influenza B viruses the HI tests employing ether-treated antigens were also evaluated. The SRH technique appears to be more sensitive for detecting protective titres of antibodies against influenza B strains and influenza A/Brazil/11/78.

Adolescent↗
PubMed1982

Enzyme-linked immunosorbent assay for the detection of antibodies to type-B influenza virus.

The Enzyme-Linked Immunosorbent assay (ELISA) has been applied to the detection of antibodies to type B influenza virus. The specificity, the sensitivity and the reproducibility of the indirect method has been evaluated. The Haemagglutination inhibition assay (HAI) was used as reference system. The results obtained at a single serum dilution show good correlation between ELISA and HAI test (r = 0.70). Reproducibility is satisfactory. Our results seem to show a better sensitivity of the ELISA test as compared to the HAI test. This feature may be useful for certain applications such as the detection of antibody responses after vaccination. The need of accurate standardization in carrying out the test is pointed out.

Adolescent↗
PubMed1982

[Antibody response to trivalent anti-influenza vaccination (inactivated virus) A/Texas/1/77 H3N2), A/URSS/90/77 (H1N1), B/Hong Kong/8/73].

Seventy-five young recruits received an intramuscular dose of anti-influenza virus vaccine containing 300 U.I. of A/Texas/1/77 (H3N2), A/URSS/90/77 (H1N1), B/Hong Kong/8/73 strains. Antibody responses were detected by HI and SRH tests: immunogenicity of the preparation was different for the individual vaccine strain in spite of the similar amount of antigenic content, and the immunity conferred by vaccine strains did not significantly extend to new influenza virus strains which prevailed in 1979/80 winter season with the exception for A/Brazil/11/78 (H1N1).

Adult↗
PubMed1982

[Antibody response to an anti-influenza vaccine of inactivated whole virus, titrated in micrograms, used in the 1980/81 season].

Antibody response of 68 healthy young-adult volunteers to A/Bangkok/1/79 (H3N2), A/Brazil/11/78 (H1N1), B/Singapore/222/79 trivalent anti-influenza virus vaccine was studied by haemagglutination inhibition and single radial haemolysis techniques. The results of this study indicate that immunogenicity of the individual components of the vaccine (10 micrograms each) varied significantly, the highest frequence of antibody response occurring for A/Brazil influenza virus strain and the lowest for B/Singapore.

Adult↗
PubMed1982

The detection of serum antibody response to type-B influenza virus in vaccinated individuals by enzyme linked immunosorbent assay.

The sensibility of the Immunoenzymatic ELISA test for the evaluation of antibody response in individuals vaccinated with inactivated Influenza vaccine was evaluated in comparison with the results obtained by the Haemagglutination inhibition test using type B Influenza virus as antigen. The rate of seroconversion obtained with the two methods versus each class of HAI titre or each interval of OD values of the prevaccine sera showed similar features. In 23% of the cases, however, sero-conversion was observed only by the ELISA test, in 5.4% of the cases only by the HAI test. It is to be assessed whether the serological response revealed by the ELISA test is fully correlated with protection against the disease.

Adult↗