Possible mode of action of mitomycin C on rat ovarian steroidogenesis.
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Biomedical subjects
Publications and source records attributed to G Majumdar.
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The activities of glucose-6-phosphate dehydrogenase, delta 5-3 beta-hydroxysteroid dehydrogenase and succinic dehydrogenase have been studied histochemically in the ovary of normal and malonate treated hens. Following malonate treatment, glucose-6-phosphate dehydrogenase and delta 5-3 beta-hydroxysteroid dehydrogenase showed increased activities while succinic dehydrogenase activity was diminished significantly. The significance of the above changes has been discussed. The ascorbic acid and cholesterol contents of the ovary were determined to get additional information about steroidogenesis in the gland under such treatment.
Suppression of delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-OHD) and glucose-6-phosphate dehydrogenase (G-6-PD) activities were observed in the rat ovarian tissues following treatment with Mitomycin C (MC), an antibiotic which depresses DNA synthesis. The same treatment also resulted in accumulation of cholesterol and ascorbic acid in the ovaries and a decrease of uterine weight. The atretic changes in the treated ovaries were judged from the activity of the lysosomal enzyme leucine amino-peptidase (LAP). The results suggest a diminution in ovarian steroid biogenesis following an alteration of DNA synthesis.
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Two nematode species (Geopetitia sp. and Diplotriaena ozouxi) and an acanthocephalan (Mediorhynchus textori n. sp.) are described. The diplotriaenid has been recorded from a new host and also from a new locality. Because of homonymy of Geopetitia chaubaudi VUYLSTEKE, 1963 with Geopetitia chaubaudi RASHHED, 1960 a new name G. vuylstekei nom. nov. is proposed for the former species. Mediorhynchus textori is characterized by lesser number of rooted hooks arranged in spirals on theproboscis. The eggs of this species bear variable floats.
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