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Biomedical subjects

G Mahuzier

Publications and source records attributed to G Mahuzier.

53 records · Page 3Linked to original sources
PubMed1982

[Determination of quinine in plasma and urine by liquid chromatography].

We propose a method to quantify quinine in biological fluids using liquid chromatography. After alkalinisation, samples are extracted with a chloroform-isoamyl alcohol mixture; in the case of urinary samples, a preliminary enzymatic hydrolysis is performed. Extracts are analyzed by adsorption chromatography. A mobile phase composed of methanol-acetonitril-ammonia in used to elute. A post-column reaction with sulfuric acid allows a fluorimetric detection. With this method concentration as low as 0.05 mg/l can be measured. Variation coefficient is about 2.5 p. cent Quinine, its metabolites and dihydroquinine are well separated; thus it is a very useful method for pharmacokinetic and metabolic studies.

Chromatography, Liquid↗
PubMed1979

High-performance liquid chromatographic determination of ketoprofen in blood and urine.

A rapid, simple determination was developed for ketoprofen in biological fluids using high-performance liquid chromatography. The method requires selective extraction of this antirheumatic medicament and an internal standard with ether from the previously acidified plasma and urine. After evaporation of the ether, the residue is taken up by methanol and analyzed by reversed-phase liquid chromatography with detection at 254 nm. A concentration as low as 0.1 microgram/ml can be determined with a 0.5-ml sample.

Chromatography, High Pressure Liquid↗
PubMed1978

[Gas chromatographic determination of diethylcarbamazine (Hetrazan) in serum of laboratory rodents (author's transl)].

After chloroform extraction in an alkaline solution, diethylcarbamazine (DEC) is measured in the presence of dipropylacetamid (internal standard), treated in the same manner, by a gas chromatographic procedure using a nitrogen detector in a 10% BDS column. Results can be easily reproduced for analysis of DEC in sera up to levels of 10 mg/1 and sensibility allows determination of approximately 0.5 mg/l levels on 50-microliter samples of serum. This method is suitable for pharmacokinetic studies.

Animals↗
PubMed1978

[Simultaneous estimation of phenobarbitone and valproic acid in the plasma by high performance liquid chromatography (author's transl)].

The authors describe a method of simultaneous estimation of phenobarbitone and valproic acid in the blood. After extraction with ether, these substances were estimated by high performance liquid chromatography. They were separated on a mu Bondapack C18 column. The detection involved a colorimetric procedure based on variation in colour of a solution of bromocresol purple. The technic used is simple, its reproducibility is satisfactory (coefficients of variation of 7.6% for phenobarbitone and 2.3% for valproic acid). The sensitivity was 13.8 mumol/l for phenobarbitone and 11.1 mumol/l for valproic acid. Parallel estimations by gas chromatography led to comparable results. This method is of interest in the supervision of anticonvulsant treatments, especially in pediatrics.

Chromatography, Gas↗
PubMed1977

[Determination of the erythrocytoplasma lithium ratio. Analytical considerations and clinical applications (author's transl)].

During eleven months the authors studied the erythrocytoplasma lithium ration in 46 patients on chronic lithium maintenance. They observed that the individual variations of this ratio are of the same magnitude in "cycloid psychosis" and in chronic schizophrenia. But, furthermore these variations are significantly more important than in neurosis. They report that this ratio increases significantly in manic depressive illness during the active manic or depressive phases, and they compare their results to those of the literature.

Adult↗
PubMedDate not supplied

Experimental filariasis of Dipetalonema dessetae in Proechimys oris: 3. Effects of parasitism on the pharmacokinetics of diethylcarbamazine.

The pharmacokinetic parameters of diethylcarbamazine were compared in the plasma and peritoneal fluid of 5 non-infected rodents. In the filarial animals, the absorption rate constant and the elimination rate constant were significantly increased. The peak plasma concentration was twofold higher in the infested rodents than the control animals, and this may account for the rapid action of diethylcarbamazine on blood-circulating microfilariae. The area under the curve was unchanged. Total clearance is slightly increased in filarial rodents. These data are explained by an important reduction of renal clearance combined with a moderate increase of extra renal clearance. High levels of diethylcarbamazine in peritoneal fluid, resulting from the excellent diffusion of the drug in filarial animals explains the activity on adult worms. But the parasitism does not affect the mechanism of distribution of the drug between plasma and peritoneal liquid.

Animals↗
PubMedDate not supplied

[Experimental filariasis in Proechimys oris by Dipetalonema dessetae. 5. Effect of parasitism on metabolism of diethylcarbamazine].

The metabolism of diethylcarbamazine (DEC) in healthy and filaria-infected Proechimys oris Rodents was investigated. DEC and 14C labelled DEC were orally administrated (100 mg/kg). The drug was quickly and intensively metabolised. About 90% of the dose administered was eliminated in the urine within 24 hours. Monodeethylation was the major pathway. The major urinary metabolites were ethylcarbamazine and its N-oxide. Traces of N methylpiperazine 2,5 dione and their N-oxide were also found. Elimination was faster in filarial than in healthy animals, but metabolites were the same.

Animals↗
PubMedDate not supplied

[Microfilaricidal action of Diethylcarbamazine on Dipetalonema dessetae in his natural host (author's transl)].

With a Filaria of Rodent whose microfilariae are periodic and diurnal, and destroyed by DEC, the authors don't observe the immediate rise of microfilaria count sometimes noted following treatment of periodic filaria during the low phase of microfilaremia. Moreover, the microfilaremia falls at 30% of the pre-treatment level after 15 mn. This may be in relation with the swift digestive absorption, as detected by the rise of the DEC serum level, whose maximum is reached within 15 mn.

Animals↗