Search PubMed⌕ Search

Biomedical subjects

G Mahrle

Publications and source records attributed to G Mahrle.

At least 73 records · Page 4Linked to original sources
PubMed1989

[Lymphomatoid papulosis resembling ecthyma. A case report].

A 54-year-old female patient recurrently developed disseminated papules, red-brown in color and partly ulcerous, which spontaneously disappeared after 3-6 weeks. Histological examination revealed dense infiltration of atypical lymphocytes with epidermotropism. Immunohistological analysis showed predominantly helper T-cells (CD 4). Accordingly, we established the diagnosis of lymphomatoid papulosis. There was no evidence of malignant lymphoma.

Biopsy↗
PubMed1989

[The atypical course of syphilis in HIV infection].

We report on 3 HIV patients showing atypical courses of syphilis. Both the history and serology of the first patient proved a recent re-infection with T. pallidum, whereas the histopathological findings corresponded to an advanced stage of the disease (S II-III). The second patient showed the clinical picture of syphilis maligna with slowly converting and slightly positive serological reactions. The third patient had a refractory syphilis and an early relapse. Our observations suggest that syphilis might take an unusual course in HIV patients. Considering our total HIV clientèle (800 patients greater than or equal to WR 2) the frequency of these atypical cases must be rated very low (0.38%).

Acquired Immunodeficiency Syndrome↗
PubMed1989

Colorimetric growth assay for epidermal cell cultures by their crystal violet binding capacity.

The application of a simple, rapid, and inexpensive colorimetric growth assay was tested for human epidermal cells subcultured in uncoated plastic dishes. Cell layers were incubated with a crystal violet (CV) solution (0.2% with ethanol 2% in 0.5 M Tris-Cl buffer, pH 7.8) for 10 min at room temperature. After rinsing with 0.5 M Tris-Cl (pH 7.8) the cell layer was dried and decolorized with a sodium-dodecylsulfate solution (0.5% with ethanol 50% in 0.5 M Tris-Cl, pH 7.8) for 60 min at 37 degrees C. The extinction of the supernatant was read at the absorption maximum of 586 nm. The protein content of attached cells as classical parameter for quantifying cell growth was strongly related to CV extinction with a correlation coefficient of r = 0.98. Furthermore, the subcellular protein binding qualities of CV were analyzed. The water-soluble protein fraction of cultured epidermal cells was separated by sodium-dodecylsulfate polyacrylamide gel electrophoresis and stained with CV. We found a staining pattern which was qualitatively very similar to that of Coomassie blue, however less intense. Keratin electrophoresis revealed an affinity of CV to the 48, 50, and 56 kD cytokeratins. In conclusion, this CV assay is a reliable and simple method for the monitoring of epidermal cell growth in cultures.

Cell Division↗
PubMed1989

Immunostaining of keratin and vimentin in epidermis: comparison of different post-embedding immunogold techniques for electron microscopy.

The present study compares different post-embedding staining methods, including conventional and low-temperature embedding techniques, for demonstration of the keratin and vimentin cytoskeleton of epidermal cells, applying commercially available polyclonal and monoclonal antibodies. Immunogold staining (5-nm particles) was performed on the following material: (a) osmium-fixed and Durcupan-embedded material, etched with various solutions; (b) aldehyde-fixed material embedded in Lowicryl K4M at 4 degrees C and -35 degrees C; (c) aldehyde-fixed material embedded in Lowicryl K11M at -60 degrees C with and without cryoprotection with glycerol. In conventionally embedded material we failed to demonstrate intermediate filaments, whereas they were stained after low-temperature embedding with Lowicryl, i.e., K4M 4 degrees C, K4M -35 degrees C, and K11M -60 degrees C. The keratin and vimentin cytoskeleton reacted exclusively with polyclonal antibodies. The best results for antigenicity as well as structural preservation were achieved by post-embedding staining of K4M -35 degrees C-embedded material. Antibodies to keratin stained the cytoskeleton in keratinocytes of all epidermal layers. Filaments were decorated in a linear alignment. Antibodies to vimentin stained the cytoskeleton of Langerhans cells and melanocytes. In these cells a linear distribution pattern of the reaction product along the filaments and an extrafilamentous cluster formation were observed, indicating staining of vimentin and a vimentin-associated protein.

Antibodies, Monoclonal↗
PubMed1988

[Wound treatment with autogenous epidermal cell expansion culture].

Sheets of autologous epidermal cells grown by expansion culture were used to cover small skin defects in seven patients with postoperative necroses, necroses due to temporal arteritis, varicose ulcers or after tangential excision of tattoos. Several transplantation techniques were used: backing of the cultured epithelia with vaseline gauze, Surfasoft, Adaptic, Silastic foil, culturing directly from Petriperm-foil. Meshed Silastic-foil proved to give the best support. Optimal take of the in-vitro epithelia (more than 80% of their surface area) was achieved only for fresh dermal wound-beds. The take was only moderate on chronic granulation tissue, but the transplants reduced the formation of fibrinous-necrotic material and favoured the formation of fresh granulation tissue.

Adult↗
PubMed1988

[Immunohistological studies of immune dermatoses on reconstituted paraffin sections. Value and comparison of different methods].

In a study on 26 patients with autoimmune diseases the immunostaining for immunoglobulins and complement in frozen sections was compared with that in formalin-fixed and paraffin-embedded sections. The formalin-fixed material of pemphigus vulgaris, lupus erythematosus, bullous pemphigoid, and dermatitis herpetiformis (Duhring's disease) was reconstituted and stained by means of the standard two-step technique (TST), the peroxidase-antiperoxidase technique (PAP), and the streptavidin-biotin method (SAB). In comparison with frozen sections, immunoglobulins and complement could also be demonstrated in formalin-fixed sections in all cases of pemphigus vulgaris and in 85% of cases of discoid lupus erythematosus, but in only 60% of cases of bullous pemphigoid or Duhring's disease. PAP and SAB proved to be about equally reliable, but TST was significantly less dependable.

Dermatitis Herpetiformis↗
PubMed1987

[Benign and malignant granular cell tumors. An immunohistochemical classification of tumor cells].

Eight benign and three malignant granular cell tumors were characterized by means of antibodies and antisera against keratin, desmin, epithelial membrane antigen, factor VIII-related protein, lysozyme, myelin basic protein, myoglobin, neurone-specific enolase, S 100 protein, myelin-associated protein (Leu 7), glial fibrillary acidic protein, vimentin, and neurofilament. All benign granular cell tumours showed positive staining of the tumor cells to antibodies against vimentin, S 100 protein, and neurone-specific enolase; myelin-associated protein (Leu 7), in contrast, was only detectable in a few tumor sections. Histogenetically the granular cells may be classified as Schwann's cells which lost their expression of laminin. The three malignant granular cell tumors showed a staining pattern significantly different from that of the benign tumours. Thus, only neurone-specific enolase was detectable in all the tumors, whereas S 100 protein and vimentin could not be demonstrated but in one and two, resp., out of three tumors.

Antibodies, Monoclonal↗
PubMed1987

[Current therapy: immunopharmaceuticals].

Immunopharmarca are classified as follows: thymus factors and hormones, lymphokines and cytokines, microbial products, drugs with potential effects on the immune system, and external immunomodulators. We discuss new drugs and substances such as Timunox, Tp-1 Serono, delimmun, Isoprenosine, recombinant interleukin 2, Immuneron, tumor necrosis factor, Sandimmun, and diphencyprone, as well as drugs already known like Levamisole, Bestatin, gold, sulfones, and sulfopyridines.

Adjuvants, Immunologic↗
PubMed1987

[Transition to cutaneous squamous cell carcinoma in 2 patients with bowenoid papulomatosis].

We report on two female patients (30 and 33-year-old) suffering from bowenoid papulosis (BP) which showed HPV-16-DNA (E.-M. de Villiers, DKFZ, Heidelberg). Both patients developed cutaneous invasive squamous cell carcinoma in the perineal and vulvar region, resp. In the first patient, the microinvasive part of the tumor was excised. Subsequent therapy with interferon-gamma resulted in complete clinical and histological remission of the BP within 4 months; 6 months after discontinuation of the therapy with interferon, however, we observed a relapse of BP. In the second case, which showed more prominent BP alterations and deeper tumor invasion, interferon-gamma did not bring on any improvement within 3 weeks of therapy; thus radical operation was performed. To our knowledge, this is the first report on the transition of BP of the outer ano-genital region into invasive squamous cell carcinoma.

Adult↗
PubMed1987

Initial lesions of HIV-related Kaposi's sarcoma--a histological, immunohistochemical, and ultrastructural study.

Kaposi's sarcoma (KS) in human immunodeficiency virus infection (HIV) has become a rather frequent manifestation of the previously rare disease with fatal outcome. Initial lesions of KS were studied by means of histopathology, immunohistology, and electron microscopy in order to define the earliest alterations. The histopathological changes of initial lesions were distinct, consisting of (1) discrete proliferation of capillary vessels, (2) dissection of collagen by proliferating spindle cells which formed slits, (3) atypical spindle cells arranged in an Indian file pattern, and (4) the lack of any inflammatory cellular infiltrate. Double staining with antibodies against vimentin and immunohistochemical markers for endothelial cells revealed that slits forming vimentin-positive spindle cells displayed laminin, factor VIII, and PAL-E. Atypical vimentin-positive spindle cells arranged in an Indian file pattern inconsistently expressed laminin and factor VIII, but not PAL-E. KS cells rarely stained with the lectin UEA I, not even in case of less advanced dedifferentiation. Electron microscopy showed gradual transformation between spindle cells forming slits and those having lost the ability to form incomplete vessel walls. The present findings support the view that KS develops from the endothelial cells of the blood vessels. The proliferation of atypical endothelial cells as early as in initial lesions and the lack of inflammation favors the primary neoplastic genesis of KS.

Acquired Immunodeficiency Syndrome↗
PubMed1987

Epidermal cell growth-dependent arylhydrocarbon-hydroxylase (AHH) activity in vitro.

Cytochrome P-450-dependent arylhydrocarbon-hydroxylase (AHH) activity and inducibility by benzanthracene (BA) was measured in cultured guinea pig and human epidermal cells. Basal AHH-activity (AHHb) in guinea pig epidermal cells was much higher than in human epidermal cells. AHHb in guinea pig epidermal cells was directly related to the labeling index and decreased to the original level between the 5th and 7th day of cell culturing. On the other hand, the induction-ratio of AHH reached its maximum level when the number of cells began to rise (proliferation phase) and remained high at day 7 of the cell culture. These results suggest a cell growth dependent activity and inducibility of carcinogen-metabolizing enzymes, such as AHH, in isolated epidermal cells.

Adult↗
PubMed1987

Combined tar-anthralin versus anthralin treatment lowers irritancy with unchanged antipsoriatic efficacy. Modifications of short-contact therapy and Ingram therapy.

In 44 patients with chronic plaque psoriasis anthralin therapy was used as high-strength short-contact therapy in a bilateral comparison of anthralin versus 5% crude coal tar-anthralin combination. These two trials were undertaken with and without ultraviolet (UV) irradiation immediately after anthralin therapy. The combined tar-anthralin therapy was significantly less of an irritant during the first 3 weeks of treatment than anthralin alone, and it did not decrease the antipsoriatic efficacy. The use of UV irradiation, either with anthralin or tar-anthralin combination, did not produce an additional therapeutic effect. These findings lead us to prefer combined tar-anthralin therapy because of its lower irritancy in comparison with anthralin alone and they show the ineffectiveness of additional UV irradiation under the conditions of this study.

Administration, Topical↗
PubMed1987

Retinoids in cutaneous T cell lymphomas.

Sixteen patients - 12 with cutaneous T cell lymphoma (CTCL), 1 with Sézary syndrome, 1 with actinic reticuloid, and 2 with parapsoriasis variegata - were treated with either a new, potent arotinoid alone or with combined etretinate (Tigason) and PUVA therapy (Re-PUVA). 92% of all patients showed a minor up to a distinct response of their skin lesions within 12.6 +/- 7.4 weeks. More than 50% of the skin lesions cleared in 67% of the patients. After discontinuation of the retinoid therapy, relapses occurred in all cases within 3-10 weeks. There was no difference between the therapeutic efficacy of arotinoid alone and the Re-PUVA regimen, but the latter was less toxic.

Antineoplastic Agents↗
PubMed1987

[Immunohistologic differential diagnosis of skin tumors in routinely embedded paraffin sections].

Thirty-nine skin tumors of epithelial, mesenchymal, and neuroectodermal origin were studied using antibodies against intermediate filaments and other cell proteins. Formol-fixed and paraffin-embedded material was reconstituted and stained with antibodies against epithelial cells (keratin, epithelial membrane antigen, carcinoembryonic antigen), mesenchymal and histiocytic cells (vimentin, alpha-1-antichymotrypsin, alpha-1-antitrypsin, lysozyme), nerve tissue (neurofilament, glial fibrillary acidic protein, myelin basic protein, myelin-associated protein, neuron-specific enolase), vessels (factor-VIII-related protein), basal cell lamina (laminin) and S-100 protein. Tumor cells displayed the same antibody pattern found in the normal cell type. It is recommended that immunotyping be started with three antibodies to allow gross classification into epithelial (keratin positive), mesenchymal (vimentin positive) and neuroectodermal (vimentin and S-100 protein positive) tumors; then, in a second step, the tumors can be subclassified by the other more specific antibodies listed above. All antibodies used in this study are commercially available and provide reliable results.

Antigens, Neoplasm↗