Search PubMed⌕ Search

Biomedical subjects

G Lupo

Publications and source records attributed to G Lupo.

At least 37 records · Page 2Linked to original sources
PubMed1994

Susceptibility of rat retina acyl-CoA:1-acyl-sn-glycero-3-phosphocholine O-acyltransferase and CTP:phosphocholine cytidylyltransferase activity to lipid peroxidation and hydroperoxide treatment.

Two enzyme activities involved in phospholipid metabolism in the rat retina were determined after in vivo and in vitro peroxidation according to several model systems. The in vivo models were based on: (i) intravenous administration of a sonicated emulsion of phospholipid and linoleate photooxidized mixture to normal rat for a period of one week; (ii) acute injection of Fe2+ solution (20 mM) or (iii) 0.5 mg of hydroperoxylinoleate into the vitreous body, and collection of retinal tissue 4 h or 4 days later, respectively. Oleoyl CoA:lysophosphatidylcholine acyltransferase activity was unchanged or exhibited significant inhibition. On the contrary, CTP:phosphocholine cytidylyltransferase activity was stimulated. By incubating in vitro the retina with: (i) Fe(2+)-ascorbate; (ii) photooxidized phospholipid mixture (0.1-5 mM) or individual phospholipid classes; (iii) hydroperoxylinoleate (0.25-2 mM), with or without Fe2+, a significant inactivation of acyltransferase (six-fold maximum loss of initial activity) and a slight stimulation of cytidylyltransferase were seen. Altogether, the results suggest that in situ oxygen radical generation by a variety of agents irreversibly perturbs enzymes and/or membrane structures in which the enzymes are inserted; these events may bea causal factor in retinal degeneration accompanying some ocular diseases.

1-Acylglycerol-3-Phosphate O-Acyltransferase↗
PubMed1994

Differential transport of docosahexaenoate and palmitate through the blood-retina and blood-brain barrier of the rat.

The permeability-surface area product (PS) of [1-14C]docosahexaenoate and [1-14C]palmitate at the blood-retina (BRB) and blood-brain barrier (BBB) was determined after in situ brain perfusion in Sprague-Dawley rats. The intracarotid injection procedure involved continuous infusion of albumin-bound fatty acids for up to 20 s. In the retina, and visual, parietal and frontal cortex, there was a significant decrease in mean PSs for docosahexaenoate compared to the palmitate group. For optic nerve and tract, superior colliculus, lateral geniculate, striatum, hippocampus and olfactory bulb, the comparison of PS values between the two fatty acid-injected groups of animals did not reveal any difference. It is suggested that the lower docosahexaenoate transport, compared to 16:0, across microvascular endothelium of the retina and other cortical regions might help explain the highest availability and selective retention of the essential 22:6(n-3) fatty acid in these nervous system structures.

Animals↗
PubMed1993

Palmitate transport through the blood-retina and blood-brain barrier of rat visual system during aging.

The permeability-surface area product (PA) of [1-14C]palmitate at the blood-retina (BRB) and blood-brain barrier (BBB) was determined after short carotid perfusion in male Sprague-Dawley rats at 4, 14 and 28 months of age. For the retina, optic nerve and tract, lateral geniculate body, visual and parietal cortex, there was no significant difference among mean PAs in any age group. For superior colliculus, frontal cortex, striatum, hippocampus and olfactory bulb, a slight but significant increase of PA values was observed between young (4-month-old) and senescent (28-month-old) rats. Our results indicate that aging does not affect influx into retina and other structures of rat visual system of the palmitate, a metabolic substrate for which carrier-mediated transport across the BRB and BBB has not been demonstrated.

Aging↗
PubMed1993

Aging does not affect the susceptibility to lipid peroxidation and lysosomal enzyme release of rat visual system structures and sciatic nerve.

The aim of the present study was to clarify the issue of lipid peroxidation operating in visual system structures and sciatic nerve of the rat as a contributing factor to senescence. In 4-, 14- and 28-month-old male rats, the amount of endogenous malondialdehyde, conjugated dienes and extractable phospholipids were all taken as indices of lipid peroxidation. In addition, the total free and released enzyme activities of four lysosomal hydrolases were evaluated. The susceptibility of all these parameters to in vitro iron-induced peroxidation was also taken as an age-related indicator of the endogenous peroxidative potential of the nervous tissues examined. Our data show that the content of malondialdehyde and phospholipids did not change in an age-related fashion. Furthermore, the susceptibility of rat visual system structures to lipid peroxidation, together with the release of lysosomal enzymes were unchanged as a function of aging. The results do not lend support to the hypothesis that an increase in overall lipid peroxidation is peculiar to the aging phenomenon of the central nervous system areas which delimit the rat visual pathway.

Aging↗
PubMed1993

Lipid peroxidation inhibits oleoyl-CoA: 1-acyl-sn-glycero-3-phosphocholine O-acyltransferase in rat CNS axolemma-enriched fractions.

The effect of phospholipid peroxidation on the acylation of lysoPtdCho (lysophosphatidylcholine) by axolemma-enriched fraction prepared from rat brain stem was investigated. After two types of peroxidative treatments, the in vitro induction of malondialdehyde and conjugated dienes formation in axolemmal membranes correlated to a shift in the ratio of saturated/unsaturated fatty acids. By using an Fe2+ (20 microM)-ascorbate (0.25 mM) peroxidation system, the residual acyltransferase activity was 55% of the initial one. No change in Km value for either oleoyl-CoA or lysoPtdCho was found, whereas a loss of 24% in Vmax was observed. After 5 min preincubation with 150 mM t-BuOOH, 70% inactivation of the acylation reaction was observed. A near suppression of enzyme activity was reached with 400 mM. The apparent Km for oleoyl-CoA decreased sharply (from 6.6 microM in control preparations to 4.1 microM in t-BuOOH-treated membranes), indicating a 2-fold increase in the enzymatic affinity for this substrate. The apparent Km for lysoPtdCho increased markedly (from 1.56 microM in the control preparations to 5.88 microM in t-BuOOH-treated membranes) whereas a decrease of Vmax (from 1.65 to 0.80 nmol/min/mg protein) for the same substrate was observed. Significant enzyme inactivation (loss of 60% of initial activity) was seen when 10 mumol of photooxidized phospholipids were preincubated with axolemmal membranes. Significant dose-dependent enzyme inactivation was brought about by addition of 10-60 mumol of peroxidized PtdEtn/100 micrograms axolemmal protein. The percent enzyme inhibition by peroxidized PtdCho at equivalent amounts was lower than that by PtdEtn.(ABSTRACT TRUNCATED AT 250 WORDS)

Acyltransferases↗
PubMed1992

HCV infection in HBsAg positive chronic liver disease.

The prevalence of anti-HCV antibodies was determined for a group of 68 patients with various forms of chronic liver disease. All patients that were anti-HCV positive but did not show signs of HBV replication had severe liver disease. We therefore suggest that HCV may be responsible for liver damage in HBsAg positive subjects when there are no evident signs of HBV replication.

Chronic Disease↗
PubMed1992

HCV and HIV infection among intravenous drug abusers in eastern Sicily.

In a study of 175 intravenous drug addicts from Eastern Sicily, 58.3% were found to be anti-HCV positive. In this population, the presence of anti-HCV was independent of HIV infection, age, duration of drug use and the practice of needle sharing. This may indicate that HCV is more readily transmitted (or spread earlier in this population) among drug addicts than is HIV.

Female↗
PubMed1992

Cytoprotective effect of copper(II) complexes against ethanol-induced damage to rat gastric mucosa.

The cytoprotective effect of various copper(II) complexes on the gastric mucosa damage induced by acute intragastric administration of ethanol was investigated. For in vitro experiments, the following copper(II) complexes were tested: Cu(II)(L-Trp)(L-Phe), Cu(II)(L-Leu)Cu(II)(L-Leu-Leu)(L-Leu), Cu(II)(L-Phe-L-Leu), Cu(II)(Gly-His-Lys), and Cu(II)(cyHis)2(ClO4)2. Inorganic copper such as CuSO4 was also tested. The free radical generating system, acting for 2 hr on cardial and fundic mucosa scrapings or mucosal microsomes, was Fe++ (20 microM)/ascorbate (0.25 mM). We found a marked inhibition to 75% of lipid peroxidation in the range 10-100 mM, regardless of whether copper was given in complexed or inorganic form. The results suggest that nontoxic copper(II)-amino acid complexes are able to neutralize oxygen-derived free radicals. In addition, copper(II) complexes suppressed membrane lipid peroxidation when mucosa homogenates were exposed to t-butyl hydroperoxide (1-20 microM) plus Fe++ (50 microM). In vivo experiments on rat stomachs, pretreated p.o. by gavage either with Cu(II)(L-Trp)(L-Phe) as paradigmatic agent or with copper sulphate at equivalent doses in the range 3-30 mg/kg body weight showed a significant decrease (30 min after 95% ethanol administration) in the number and severity of mucosal hemorrhagic lesions. In the gastric mucosa scrapings of copper-treated rats after ethanol exposure, we found that malondialdehyde and conjugated diene levels were unchanged compared to those of untreated controls; five enzyme activities released from lysosomes were near control values. In isolated mucosal cells, whether or not pretreated with 200 microM solution of either Cu(II)(L-Trp)(L-Phe) or CuSO4, the release of cathepsin D activity was also unmodified. The results suggest that the cytoprotective effect of Cu(II) complexes against ethanol-induced mucosal lesions was not associated in vivo to lipid peroxidation.

Amino Acid Sequence↗
PubMed1990

Free and antibody-complexed antigen and antibody profile in apparently healthy HIV seropositive individuals and in AIDS patients.

The pattern of free and antibody-complexed HIV antigen and the antibody profile were investigated retrospectively in 305 serum samples taken from 22 AIDS patients before and during the development of AIDS and from 40 apparently healthy seropositive individuals. Most AIDS patients were found positive for both free and complexed antigen and had high gp41 antibody titres but low or undetectable p24 antibody. Four different patterns of HIV antigenaemia were observed: 1) positive for both free and complexed antigen; 2) negative for free HIV antigen at first, but always positive for complexed antigen; 3) positive for free antigen without complexed antigen; and 4) negative for both free and complexed antigen. The development of immune complexes preceded the appearance of free antigen and might reflect the ongoing viral replication with antigen excess and binding of anticore antibodies. No correlation was found between the development of AIDS symptoms and either the duration of free antigen positivity or the level of antigenaemia. A different pattern was observed in apparently healthy seropositive individuals: 90% of whom had high antibody titres to p24 and gp41 and were persistently negative for free and complexed HIV antigen. This study demonstrates that testing HIV markers in sequentially collected serum samples from HIV seropositive individuals is a useful and simple tool for early identification of persons at risk of developing AIDS.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Effect of clindamycin on adherence of coagulase-negative Staphylococcus to surgical nets.

The authors studied the effect that a subinhibitory concentration of Clindamycin has on adherence of coagulase negative S. epidermidis to surgical nets. Clindamycin, as expected, interferes with the adhesivity to the nets of coagulase-negative S. epidermidis slime producing when sub-MICs are used. The documentation obtained by scanning electron microscope, after treatment of the strains with doses of Clindamycin equal to 1/6 of the MIC after 3 and 6 hours, confirms the anti-adherent effect of the antibiotic.

Bacterial Adhesion↗
PubMed1989

Neutralizing antibody and clinical status of human immunodeficiency virus (HIV)-infected individuals.

An assay based on inhibition of cytopathic effect of human immunodeficiency virus (HIV) strains in Molt 4 cells was developed to quantitate neutralizing antibodies (NA) in sera of HIV-infected individuals. The assay was specific and gave results comparable to those obtained by the inhibition of immunofluorescence (IFI) and reverse transcriptase (RT) activity. Attempts were made to correlate the presence and the antibody titres with the clinical status of HIV-infected individuals classified according to Walter Reed staging classification scheme. NA titres correlated inversely with the stage of HIV infection: Compared with acquired immunodeficiency syndrome (AIDS) patients, HIV-infected subjects at stage WR1 had significantly higher NA titres. Moreover, a decrease in NA titre in relation to clinical deterioration was noted in sequential sera of eight of 11 AIDS patients, retrospectively examined, for NA. The symptomless subjects showed either the same level of NA or a trend towards an increasing antibody titre with time. Different isolates of HIV strains showed a variability in the extent of sensitivity to neutralization by sera obtained from different HIV-infected individuals.

Acquired Immunodeficiency Syndrome↗
PubMed1989

No evidence of HIV 2 infection in Sicily.

A seroepidemiological survey was carried out with the aim of establishing whether or not HIV 2 infection could be detected in Western Sicily. Two groups of sera were tested by EIA and WB assay against HIV 1 and HIV 2. 200 sera taken from North African immigrants in the Mazara del Vallo area, and 297 samples taken from AIDS-high risk groups individuals living in Palermo. None of the North African sera was positive to HIV 1 and/or HIV 2 by either techniques. 118 of the 297 sera from Palermo were HIV 1 positive by both techniques; 69 thereof also reacted to HIV 2 by EIA. Only six of these were confirmed by WB assay, showing a clear reactivity against the 140 and 105 HIV 2 glycoproteins. However, all six sera were considered merely cross-reactive to HIV 2, since none of them had neutralizing antibody to HIV 2 and the cross reactivity to glycoproteins of HIV 2 could be removed by absorbing them with HIV 1 infected cells. It seems therefore that HIV 2 has not yet reached our area. Early detection of the possible presence of this virus in our country could be obtained by improving both the surveillance and the effectiveness of laboratory tools for HIV 2 diagnosis.

Acquired Immunodeficiency Syndrome↗
PubMed1988

Main routes of transmission of human immunodeficiency virus (HIV) infection in a family setting in Palermo, Italy.

A cross-sectional seroepidemiologic study was carried out on household contacts and sexual partners of human immunodeficiency virus (HIV) antibody-positive intravenous drug abusers in Palermo, Italy, in 1985 to evaluate factors that influenced HIV transmission. A total of 43 index cases, 36 spouses or heterosexual partners, 28 children, and 55 adult household members were enrolled. None of the household members without sexual contact, who had shared items and facilities and had interacted with the index cases, contracted HIV infection. However, six of 36 sexual partners had antibodies to HIV. It was observed that the risk of HIV infection was significantly associated with the frequency of sexual intercourse with the seropositive partner. Four children were also found to be infected: two had acquired immunodeficiency syndrome-related complex, and the other two were clinically and immunologically normal. Furthermore, one other child had evidence of passive transfer of maternal antibodies. The infection was confined to the younger children (ranging in age from eight months to three years). The data support a high rate of vertical transmission from mother to infant, an intermediate rate of transmission to sexual partners, and no transmission attributable to household contact.

AIDS-Related Complex↗
PubMed1988

Further isolation of HIV strains in Palermo: some biological properties of the new isolates.

21 HIV strains were isolated from peripheral mononuclear cells of 31 adult and pediatric patients with AIDS or ARC and 10 apparently healthy HIV seropositive individuals. Viral isolation rate was correlated to the clinical status of patients, being very high in patients with AIDS or ARC, and lower in apparently healthy individuals. 4 isolates were propagated in cultures of a T-cell continuous line (Molt 4) and their biological properties were compared. The capacity to establish a chronic infection on Molt 4 cells and the extent of cytopathic effect of the infected cultures varied among the different strains. Moreover, differences in endpoint titrations of the supernatants and variable effects on cell multiplication and viability of Molt 4 cells were also observed. Our results further substantiate the existence of a wide heterogeneity of HIV strains.

AIDS-Related Complex↗