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Biomedical subjects

G Liberge

Publications and source records attributed to G Liberge.

At least 19 recordsLinked to original sources
PubMed1985

[Diagnostic and therapeutic use of human anti-D (Rho) monoclonal antibodies. Evaluation and perspectives].

Human monoclonal antibodies will be essential in medicine. They are valuable tools for biological diagnosis and therapeutics. Our model, human monoclonal antibodies directed against the Rhesus D antigen can be used for the determination of the Rhesus D phenotype and for the suppression of Rh(D) immunisation in women. These new products require new procedures of preparation, new regulations for the quality controls, which will be discussed in this paper.

Animals↗
PubMed1983

H-deficient blood groups of Reunion island. II. Differences between Indians (Bombay Phenotype) and whites (Reunion phenotype).

Two variants of recessive, H-deficient nonsecretor individuals (h/h, se/se) were identified on Reunion Island: (1) H-negative individuals corresponding to the classical Bombay phenotypes (OhO, OhA, OhB, OhAB) who lack completely the H antigen on their red cells; all of them were Indian and had strong anti-H antibodies reacting with normal O and Oh red cells from whites; and (2) H-weak individuals (Oh, Ah, Bh, ABh). This phenotype represented the majority (85%) of the H-deficient phenotypes on Reunion Island, and all of them were white. They had only a weak expression of the H antigen and showed small but detectable amounts of ABH antigens on their red cells. Their anti-H antibodies reacted with normal O erythrocytes, but failed to react with Oh red cells, regardless of the ethnic origin of the donor. They were all from the same geographical area on the Island (Cilaos) and showed homogeneous titers of anti-H antibodies in sera. We propose to call this particular variant of weak H phenotype, belonging to the so-called para-Bombay series, Reunion.

ABO Blood-Group System↗
PubMed1981

A family demonstrating the independence between Lutheran and Auberger loci.

The family presented here demonstrates the absence of a close relationship between the Auberger loci on one hand and the Lutheran and secretor loci on the other. This absence of a close relationship between the Lutheran and Auberger loci is important in understanding the inhibition mechanism of the Au antigen when in the presence of the dominant In(Iu) allele.

Alleles↗
PubMed1980

Different H deficient phenotypes present in one kindred.

Two different H deficient phenotypes are observed in one kindred. Three of them in two generations of a same family appear as Bombay like. In the other branch of the kindred, an Hz phenotype (as described in the editorial of this issue) is observed. The most simple explanation is that the three Bombay like phenotypes correspond to Hz ABH non secretor (Hz sese) individuals, this being indistinguishable from a true Bombay. The high level of I antigen in the plasma of the three Bombay like (as observed in Hz in contrast to true Bombay) could favour such an hypothesis. According to ORIOL'S new hypothesis [5], III2, III3 and IV2 would genetically be hh sese, III8 would be hh Se and the h Se would therefore be a recombining haplotype (the original haplotype being h se).

ABO Blood-Group System↗
PubMed1980

Relationship between I and H antigens. II. Study of the H and I deficient phenotypes.

H and I substances are present in the plasma and the saliva. In the plasma there is no quantitative relationship between H and I substances, whereas in the saliva only non-secretors produce I substance [shown by anti-Is serum, (Sti.)]. The study of I and H substances in the plasma and the saliva of H and I deficient subjects gives a better understanding and enables the completion of the classification of these rare phenotypes. The results also allow the proposal of a synthetic scheme of Is and H substances in the saliva.

Agglutination↗
PubMed1978

[A family with an "Hm" phenotype transmitted over 3 generations].

Hm phenotype represents a dissociation between a normal salivary expression of H substance and a very weakened expression of the antigen on red blood cells. Genetic analysis of the reported family reveals a dominant inheritance: Some members (Marie K..., Francette, Carmen) present a phenotype marked by a normal H enzyme but a deficient H antigen in erythrocyte membrane. Others Alice, Mathilde) have no expression of A1 antigen due to H substrate deficiency. H substance in salivary secretion is normal. In the other branch of this pedigree without consanguinity, Herbert presents an H substance deficiency, though quite different, as A1 antigen is expressed. In this family, Hm phenotype can be explained, without resorting to a Zm allele, by the expression of an exceptional allele at the H locus (like Am is an ABO allele). This hypothesis supports the possible polymorphism of H locus.

ABO Blood-Group System↗
PubMed1976

Cis AB blood groups. Immunologic, thermodynamic and quantitative studies of ABH antigens.

Fifteen samples of cis AB bloods belonging to six unrelated families were tested by serological and thermodynamic assay techniques. The B and H antigens of cis AB bloods differ significantly from those of trans AB bloods. Differences were found among unrelated samples, but identical results were obtained within a given family : this could mean that there had been as many mutations as there were families.

ABO Blood-Group System↗
PubMed1975

[The Cis AB complex of the ABO system].

Members of six unrelated families from Japan, France, Belgium and Poland were studied in parallel. Major immunological features characteristic of the phenotype produced by the Cis AB complex are the following: 1) The red cell A reactivity is close to normal, is beyond the values of agglutination scores by Helix and by anti-A from B; likewise, with percent agglutination measurements, A reactive appears hiher than that of A2B cells; one sample only is slightly detected by anti-A from Dolichos. 2) The B reactivity, on the contrary, is lower than that of normal AB cells. A single sample is detected by anti-B from A1. All samples are well detected by anti-B from AW, Aend, Ax, Am but none is detected by anti-B from ABx, Cis AB, or by an auto-anti-B. Under standard conditions, percent aggutination is around 80, very close to that of normal AB cells, thus differentiating Cis AB from AB3 (some of which only reach this figure), and from ABx which are very far from this value. 3) An abnormally high reactivity to anti-H antibody is observed, higher than that of normal A2B, similar to that of A2 red cells. 4) Among secretors, A substance is found to be normal or in excess, H substance is in excess, while B substance is only detected by Cis AB red cells inhibition. 5)An anti-B antibody was identified in the samples studied; however, we recently received from Germany a Cis AB samples, the serum of which did not contain anti-B antibody. By these main characteristics, the studied samples seem to be identical; however, agglutination kinetics and thermodynamic methods show that they differ by their reaction with a same anti-B antibody in standard conditions. The reactive structures of the various samples are indeed different from one family to another. The main point is that identical values were observed in all samples within a same family. Thus, the various Cis AB can be considered as different families mutants.

ABO Blood-Group System↗
PubMed1975

[Cold agglutinin with anti-B specificity in an A1B subject].

A high titre cold autoagglutinin with anti-B specificity was found in the serum of an A1B group individual. It was associated with a low titre anti-I. This anti-B agglutinated most cells having a B antigen (normal B, A1B, A2B, from adult and cord bloods, B3), but failed to agglutinate Bx Cis-AB and Bh cells. Nevertheless, all these cells absorbed the anti-B SER at low temperatures. Fixation elution tests were also positive on the patient's cells and three consecutive absorptions on these cells completely removed the anti-B activity. The Coombs test was positive with anti-complement anti-globulin when the cells were sensitized by the serum at 4 degrees C. It was negative when the cells were sensitized at 37 degrees C. The patient did not show any sign of haemolysis. The anti-B was a IgM Kappa. Its reaction with normal B cells had an enthalpy change of - 36-000 cal./mole, i.e. very different from O ANd A individuals, but similar to that of the erythrocytic I antigen - anti-i antibody reaction. Quantitative measurements showed the erythrocyte B antigen similar to that of control A1B cells.

ABO Blood-Group System↗
PubMed1975

A cold auto-agglutinin of anti-B specificity in an A1B patient.

A high titre cold auto-agglutinin with anti-B activity was found in the serum of an A1B group individual. The B/anti-B reaction was thermodynamically investigated and quantitative analysis of the patient's B antigen revealed no abnormality.

ABO Blood-Group System↗