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Biomedical subjects

G Lavergne

Publications and source records attributed to G Lavergne.

At least 37 records · Page 2Linked to original sources
PubMed1977

Homocytotropic antibodies (IgE) to Neisseria gonorrhoeae in the rat and a cross-reactivity of heterologous gonococcal strains.

Outbred Wistar rats were immunised with a single intraperitoneal injection of a mixture of 30 mg of A1(OH)3 and 100microng of gonococcal zeolite antigen (ZA). Ten days after immunisation, ZA prepared from T1 and T4 colonies of Neisseria gonorrhoeae strain GC6 (GC6-T1 ZA and GC6-T4 ZA) were able to elicit a reaginic (IgE) response which declined to low levels by 35 days. There was a significant amount of histamine release from the mast cells of actively-sensitised rats on challenge with a specific gonococcal antigen. The antisera heated at a temperature of 56 degrees C for four hours failed to elicit a passive cutaneous anaphylaxis (PCA) reaction, indicating a lack of IgGa antibody. In addition antisera to GC6-T1 ZA gave a positive PCA reaction with GC6-T4 ZA and ZA prepared from T1 colonies of six heterologous gonococcal strains suggesting that these strains of N. gonorrhoeae share common antigenic determinants.

Animals↗
PubMed1977

The effect of hyperimmunization with Neisseria gonorrhoeae on the presence of gonococcal antibody in serum, tissues, and secretions of the rabbit.

Antibody responses in sera, tissues, and secretions of the urogenital tract and lower respiratory tract of rabbits hyperimmunized with Neisseria gonorrhoeae were examined. Antibody was detected by passive hemagglutination, whole-cell agglutination, bentonite flocculation, and in some cases immunodiffusion-in-gel. Immunization of rabbits either intravenously or intramuscularly resulted in the presence of gonococcal antibodies in the sera, spleens, and tissue of the urogenital tract (vagina, cervix, uterus, and fallopian tubes). Gonococcal antibody was also found in secretions bathing the mucosa of the urogenital tract and lower respiratory tract. Antibodies were not detected in sera, tissues, and secretions of non-immunized rabbits. The spleen was shown to synthesize gonococcal antibody in vitro in response to hyperimmunization. Tissues of the urogenital tract did not appear to synthesize gonococcal antibody thus suggesting and antibodies present in secretions of the urogenital tract were derived mainly from serum.

Agglutination Tests↗
PubMed1976

Iron as a replacement for mucin in the establishment of meningococcal infection in mice.

Experimental infection of mice with Neisseria meningitidis was established by the injection of the bacteria suspended in solutions of various iron compounds. The progressive and fatal infection caused by otherwise non-lethal doses of organisms was produced in these mice after prior injection with ferrous sulphate or concomitant injection with iron sorbitol citrate or iron dextran. Reduction in LD50 to levels at least comparable to those obtained in the mucin challenge system was achieved; in some serogroups of N. meningitidis the LD50 was decreased more than a million fold. The results suggest that iron, which is a component of hog gastric mucin, is a factor involved in the establishment of meningococcal infection in mice. Use of iron compounds as injection medium offers a more advantageous system than mucin, since controlled administration of chemically defined substances occurs.

Animals↗
PubMed1975

The chick embryo in studies of virulence and immunity with Neisseria Gonorrhoeae.

Intravenous inoculation of 11-day old chick embryos with Neisseria gonorrhoeae has confirmed the original observation of Bumgarner and Finkelstein that T1 and T2 gonocci are significantly more virulent than T3 and T4. Pili do not seem to be solely responsible for this virulence, since elimination of pili did not effect either the viability or the virulence of N. gonorrhoeae. In neutralization studies, there was only one log difference between the ability of normal and hyperimmune rabbit serum to protect the embryo against gonoccocal infection. However, when mouse sera were used in the same chick embryo system a clear difference was noted between the protective activity of normal serum and that antisera elicited in mice by purified gonococcal antigens. It is suggested therefore that mouse antisera be used in this chick embryo model for the assay for gonococcal antigens.

Animals↗