[Biochemical and rheological data in sputum. I. The biochemical definition of mucins in sputum (author's transl)].
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Amino Acids↗
Biomedical subjects
G Lamblin
Publications and source records attributed to G Lamblin.
[Study of mucins of two sinus mucoceles (author's transl)].
Glycoproteins were isolated in the contents of two sinus mucoceles by ionic exchange and gel filtration chromatography. These glycoproteins are of the mucin-type and characterized by their richness in carbohydrate, a low amino acid content with a strong proportion of hydroxy amino acids. However, they differ largely by their peptide axis, the length of the carbohydrate chain and their acidity, which is in relation with the presence of sialic acid residue and of sulfate groups. The least acidic mucins are the richest in sialic acid residue and in threonine but have the shortest carbohydrate chains while the most acidic are rich in sulfate, richer in serine and have longer carbohydrate chains. The wall of these two mucoceles has only one type of cell capable of synthetizing the glycoproteins: the epithelium goblet cells revealed by the PAS and the alcian blue at different pH. Glandular formations have never been found in the chorion.
Amino Acids↗
[Comparative study of bronchial mucins isolated from the sputum of patients suffering from cystic fibrosis or other chronic bronchial diseases (author's transl)].
Most of the mucins isolated from the sputum of patients suffering from cystic fibrosis are acidic. Acidic mucins from a child suffering from cystic fibrosis were degraded by alkali treatment. Analysis of the degradation products demonstrated the wide heterogeneity of the carbohydrate chains linked to the mucin polypeptide moiety. However, this heterogeneity of carbohydrate chains is probably not restricted to mucins from patients with cystic fibrosis. Bronchial mucins were prepared either from the sputum of different patients belonging to blood group O or B and suffering from cystic fibrosis, chronic bronchitis and other chronic bronchial diseases, or from bronchial washings performed in macroscopically healthy area of the bronchial tree of subjects belonging to blood group O. The chemical composition of each mucin fraction was established and an average carbohydrate chain length was estimated. Acidic mucins isolated from the sputum of two children suffering from cystic fibrosis were more sulfated than sialylated and their average carbohydrate chain length was relatively large. These characters were not specific for cystic fibrosis since they were also found in acidic mucins of two children suffering from other bronchial diseases. Most of the acidic mucins isolated from the sputum of three adults suffering from chronic bronchitis were more sialylated than sulfated and had a relatively short average carbohydrate chain length. The sputum of these patients also contained a variable proportion of neural or weakly acidic mucins. The mucins isolated from bronchial washings performed in macroscopically healthy areas of the bronchial tree were acidic molecules whose acidic characteristics and average carbohydrate chain length were about the same as for acidic mucins from patients with chronic bronchitis.
Adult↗