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Biomedical subjects

G L Wantzin

Publications and source records attributed to G L Wantzin.

At least 37 records · Page 2Linked to original sources
PubMed1987

Sequential immunophenotypic study of lymphoid infiltrate in allergic and irritant reactions.

Sequential biopsies (4-72 h) of early allergic and irritant patch test reactions have been examined immunohistologically for reactivity with 19 monoclonal antibodies against antigens on lymphoid cells in order to investigate the nature/origin of the infiltrating lymphoid cells and assess their state of activation/proliferation. The composition of the infiltrates was similar in allergic and irritant reactions and consisted of T-lymphocytes of helper/inducer types in association with T-cell accessory cells, i.e., Langerhans cells and HLA-DR-positive macrophages. No differences in expression of T-cell or macrophage associated antigens were seen in early as opposed to late biopsies. In contrast, the proportion of cells positive for markers associated with activation (interleukin-2 receptor) or proliferation (transferrin receptor, the Ki-67 nuclear antigen) of lymphoid cells was found to increase with time in both types of reaction. These data substantiate the view that T-cell immune reactions are implicated in both allergic and toxic patch test lesions, and indicate that the lymphocytes in the infiltrates are activated and proliferate.

Adult↗
PubMed1987

Extracutaneous spreading with fatal outcome of mycosis fungoides in a patient treated with ciclosporin A: a word of caution.

A patient with mycosis fungoides in the tumor stage was treated with ciclosporin A orally in daily doses of 5 mg/kg body weight for 5 weeks. Three weeks after cessation of treatment the patient died with dissemination of the cutaneous lymphoma to the lungs, myocardium, liver, pancreas, kidneys, adrenals, and stomach. Treatment with ciclosporin A may have contributed to the death of this patient.

Cyclosporins↗
PubMed1987

Oral retinoids in mycosis fungoides and Sézary syndrome: a comparison of isotretinoin and etretinate. A study from the Scandinavian Mycosis Fungoides Group.

Thirty-nine patients with mycosis fungoides in various stages or Sézary syndrome were treated with isotretinoin and 29 with etretinate as single drug therapy. Complete remission within 2 months was obtained with isotretinoin in 8 cases (21%) and partial remission in another 15 cases (38%). Etretinate induced complete remission in 5 cases (21%) and partial remission in 11 (46%). Only 1 case with Sézary syndrome went into partial remission. The first sign of remission occurred in 2 to 4 weeks. During continued treatment remissions could not always be maintained. Isotretinoin and etretinate were considered to be of equal potency in the treatment of mycosis fungoides.

Drug Eruptions↗
PubMed1986

Occurrence of human T cell lymphotropic virus (type I) antibodies in cutaneous T cell lymphoma.

Of 315 patients from Scandinavia and West Germany with cutaneous T cell lymphoma, thirty-six (11.4%) had specific antibodies reactive against human T cell lymphotropic virus type I (HTLV-I). Among the HTLV-I antibody-positive patients, one had Sézary syndrome; five, mycosis fungoides, plaque stage; sixteen, mycosis fungoides, plaque stage with nondiagnostic histologic features; and three, lymphomatoid papulosis. All the patients from the Copenhagen area had several samples taken during the course of their disease, but the HTLV-I antibody titer was unaltered independent of the clinical stage the individual patient had at the time of the study. Eighty-three patients with non-Hodgkin's lymphoma were tested for HTLV-I antibodies, and all except two showed negative results. The finding of specific antibodies reactive against HTLV-I in cutaneous T cell lymphoma suggests that a retrovirus related to HTLV-I plays an important role in the pathogenesis of cutaneous T cell lymphoma.

Antibodies, Viral↗
PubMed1986

T-cell growth factor receptor (Tac-antigen) expression in cutaneous lymphoid infiltrates.

A total of ninety-four biopsies from normal skin, benign dermatoses, and lesions of human T cell lymphotropic virus type I (HTLV-I)-positive and -negative cutaneous T cell disorders have been examined immunohistologically for reactivity with anti-Tac (monoclonal anti-T cell growth factor receptor). Tac-positive T cells were plentiful in approximately half the specimens, and they were equally frequent in benign and malignant disorders and in HTLV-I-positive and -negative cutaneous T cell disorders. Furthermore, in diseased skin, reactivity with anti-Tac was not confined to T lymphocytes, since it was also a property of dermal macrophages and Langerhans cells, as shown by examination of serial and/or double-stained sections. Comparison with other markers linked to T cell activation and proliferation (i.e., HLA-DR, OKT10 antigen, transferrin receptor, and the Ki-67 nuclear antigen) showed that Tac-antigen expression did not correlate clearly with any of these markers. These data indicate that anti-Tac forms a useful supplement to other monoclonal antibodies for the identification of activated cell subsets in cutaneous lymphoid infiltrates, but they also show that the use of this reagent on cutaneous biopsy specimens is of only limited diagnostic value.

Antibodies, Monoclonal↗
PubMed1986

Syphilis in HTLV-III infected male homosexuals.

Out of 100 male homosexual persons screened for AIDS, 54 were found to be HTLV-III antibody positive. Of these, 34 (63%) had a positive history of syphilis, as compared to 16 (35%) of the 46 HTLV-III antibody negative persons (P less than 0.01). In the HTLV-III antibody positive group 12 (22%) had had more than three syphilis infections as compared to none in the negative group (P = 0.02). The frequency of a previous primary, secondary, and latent syphilitic infection was different in the two groups. During the 1970s and early 1980s the incidence of syphilis in the U.S.A. and in Denmark was increasing, but from 1982 the incidence decreased markedly, possibly a consequence of the fear of AIDS, which appeared in 1981-82. It is speculated that the high frequency of syphilis among homosexual men might have been a co-factor for the acquisition of the HTLV-III infection.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Expression of a cell-cycle-associated nuclear antigen (Ki-67) in cutaneous lymphoid infiltrates.

Cryostat sections of skin biopsies from 83 patients with benign or malignant cutaneous lymphoid infiltrates were examined immunohistologically for reactivity with Ki-67 (a monoclonal antibody recognizing a nuclear antigen expressed by cycling cells) using the APAAP immunoalkaline phosphatase labeling method and a double immunoperoxidase/alkaline phosphatase staining technique. Ki-67-positive neoplastic lymphocytes were plentiful in all large-cell lymphoma cases and in one-third of the small-cell lymphomas. Some benign disorders (patch test biopsies, cutaneous lymphocytoma, lichen planus) also contained many Ki-67-positive lymphocytes. These data indicate the value of using Ki-67 to assess the proliferative capacities of the lymphoid cells in cutaneous infiltrates. Use of this technique in the future may have important prognostic and therapeutic implications in the management of cutaneous lymphoid malignancies.

Antibodies, Monoclonal↗
PubMed1986

Photosensitive dermatitis with actinic reticuloid syndrome: an immunohistological study of the cutaneous infiltrate.

Cryostat sections of skin biopsies from five patients with chronic photosensitivity dermatitis with actinic reticuloid syndrome (PDAR) have been examined immunohistologically by the alkaline phosphatase:anti-alkaline phosphatase staining technique using a panel of 24 monoclonal antibodies against lymphoid cells and their subsets. The lymphoid infiltrates in all cases had an essentially identical cellular composition, containing a mixture of T-lymphocytes, T-cell accessory cells (Langerhans cells) and other types of HLA-DR positive dermal macrophages. In two patients there was an excess of T-helper/inducer cells relative to T-suppressor cells, while in the other three patients the numbers of T-cells in these two subsets were approximately equal. Many of the infiltrating T-cells expressed activation (HLA-DR, interleukin-2 receptor) or proliferation (the Ki67 nuclear antigen, transferrin receptor) associated markers. These data indicate that a T-cell immune response is operative in cutaneous PDAR lesions.

Aged↗
PubMed1986

Immunohistological reactivity pattern of the anti-cutaneous T-cell lymphoma antibody BE2.

It has been reported that the monoclonal antibody BE2, raised against leukaemic T cells, reacts specifically with malignant lymphoid cells and represents a valuable reagent for the early identification of cutaneous T-cell lymphomas. To test this hypothesis, a comprehensive range of nodal and cutaneous biopsies have been examined immunohistologically using single and double immunoenzymatic and immunofluorescent staining methods. BE2 showed a broad range of reactivity, consistently labelling normal endothelial cells, B-lymphocytes in mantle and marginal zones, T-lymphocytes in the paracortex of lymph nodes and medulla of thymus, as well as a variety of different macrophage types, including Langerhans cells and dermal macrophages. Furthermore, although BE2-positive T-lymphocytes were most frequent in malignant lymphomas, they were also found in four of 19 benign dermatoses. We conclude that BE2 is neither T-cell nor tumour-cell specific, and that use of this reagent on tissue sections is unlikely to improve the diagnosis of cutaneous lymphomas.

Antibodies, Monoclonal↗
PubMed1986

Reactivity of T lymphotropic retrovirus antibody (12/1-2) in man: comparison of epidermis with other epithelial cells.

The reactivity of a monoclonal antibody against human T lymphotropic retrovirus (antibody 12/1-2, recognising the HTLV-1 p19 internal core viral protein) with benign and malignant cutaneous biopsy specimens was examined and compared with results obtained on normal skin, on various other human cells and tissues, and on immunoblotted extracts of tonsil squamous epithelium. In keeping with previous studies, 12/1-2 labelled a proportion of the thymic epithelial stroma and the entire layer of basal cells in stratified non-keratinized and keratinized epithelium. Furthermore, antibody 12/1-2 reacted with basal cell carcinomas and showed an essentially identical staining pattern in normal skin, cutaneous T cell lymphomas, and a range of benign dermatoses. The dot blot preparations showed that 12/1-2 recognised an antigen associated with keratin intermediate filaments. These data indicate that antibody 12/1-2 forms a useful marker for subsets of epithelial cells, which presumably participate in T cell education, and that a range of cutaneous disorders of widely different aetiology show no abnormalities in epithelial expression of this antigen.

Antibodies, Monoclonal↗