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G L Moore

Publications and source records attributed to G L Moore.

At least 37 records · Page 2Linked to original sources
PubMed1987

Post-thaw storage at 4 degrees C of previously frozen red cells with retention of 2,3-DPG.

Fresh human blood was collected in CPD, frozen by either the Meryman or the Valeri high glycerol technique, and stored at -80 degrees C. Later the red cells were thawed, deglycerolized by the appropriate technique and resuspended in either saline-glucose wash solution or an additive solution containing ascorbate-2-phosphate, adenine, glucose (dextrose), mannitol and sodium phosphate. The cells were stored at 4-6 degrees C for 21 days and assayed weekly for ATP, 2,3-DPG, pH, P50, glucose utilization and lysis. The additive solution maintained red cell 2,3-DPG at fresh blood levels for 3 weeks and maintained ATP levels sufficiently well to suggest good red cell viability for 21 days. There was no difference in results between the Meryman or the Valeri freezing methods if sodium phosphate was used with the saline-glucose wash solution in the Valeri method. If this additive solution is coupled with sterile deglycerolization techniques, 3 weeks of post-thaw red cell preservation would be practical. Using this additive solution would make frozen blood a reasonable source of red cells for emergency needs in both military and civilian blood banking.

2,3-Diphosphoglycerate↗
PubMed1987

Additive solutions for better blood preservation.

For several decades the standard blood preservative solution consisted of citrate, dextrose, and, later, phosphate (ACD and CPD). In 1978 a new solution containing adenine (CPDA-1) was introduced to permit extension of red cell shelf life from 21 to 35 days. The success of CPDA-1 and the high percentage of blood units processed into components (estimated 87% in 1983) have stimulated a burst of research and development activity to develop improved red cell preservation systems. Most of these systems have taken the form of "additive solutions" in which the blood is drawn in CPD or CP2D and processed into components. Then the packed red cells are stored by addition of a solution customized for their preservation. This review evaluates these additive solutions in detail. Innovative systems for pH control (buffers and resins) have also been explored. Our review concludes with discussion of the safety aspects of new preservative solutions and the methodological problems of evaluating these solutions.

Blood Preservation↗
PubMed1981

Red cell ATP and 2,3-diphosphoglycerate concentrations as a function of dihydroxyacetone supplementation of CPD adenine.

Units of CPDA-1 whole blood were subdivided and each treated with additions of dihydroxyacetone (DHA) to give final concentrations from 0 to 80 mM. The 'optimum' concentration of DHA to maintain 2,3-diphosphoglycerate (2,3-DPG) with minimal loss of ATP during 42 days of storage appeared to be 30 mM of DHA. With this formulation, red cell 2,3-DPG concentrations rose to 130-140% of normal by 14 days and then decreased in a near-linear manner to 50-60% normal by 42 days, while maintaining adequate ATP levels. In addition, packed red cells were prepared form CPD fresh blood and treated with adenine, glucose, and various concentrations (0-80 mM) of DHA. The cells also responded most favorably to 30mM DHA, although the response was not as positive as whole blood. This concentration of DHA produced nearly 100% maintenance of 2,3-DPG at 14 days with subsequent fall to 30% of normal by 42 days.

2,3-Diphosphoglycerate↗
PubMed1981

Adenine in blood preservation.

The recent Food and Drug Administration (U.S.) approval of a new blood preservative (CPDA-1) which contains adenine not only introduces a new blood product into the American blood banking system, but also heralds the advent of novel approaches to blood product preservation. The use of adenine to effect maintenance of red cell adenosine triphosphate (ATP), and hence to prolong storability, has a well-founded biochemical rationale. Effects of adenine on red cell metabolism are generally well understood, but effects on other blood components have not been fully delineated. The efficacy of adenine preservatives in enhancing the duration of red cell storage appears to outweigh the small risk of toxicity from free adenine. Clinical use of millions of units of adenine-preserved blood in Europe during more than a decade has resulted in only one report of possible adenine toxicity. Marginal acceptability of 24-hr 51Cr red cell recovery of packed red cells stored for 35 days in CPDA-1 has stimulated development and evaluation of an improved preservative (CPDA-2) which may extend blood storability beyond 35 days. A heightened awareness of the hematological consequences of prolonged storage has come with the extension of blood storage beyond 21 days. The concepts of component-specific preservation systems and optimal preservation systems have emerged as a result of experimentation on adenine preservatives. While the influence of adenine preservatives on American blood banking is yet to become manifest, the ultimte impact of adenine on blood preservation may be the development of novel systems which optimally preserve specific blood components at the option of the user.

Adenine↗
PubMed1978

The adult psychiatrist in the medical environment.

To function effectively in the medical environment a psychiatrist must be comfortable with the skills and values of medicine. He or she must nurture his or her original knowledge in this area with an ongoing learning program. Certain problems are uniquely encountered in this environment. The psychiatrist must understand that various psychiatric disorders may be masked by or may mask somatic complaints. He or she must expect to deal with threatened and threatening patients. Most important, the psychiatrist must understand that to be effective he or she must make a commitment to involvement.

Alcoholism↗
PubMed1976

Psychiatric aspects of chronic renal disease.

Life-extending treatment for end-stage renal disease is now accepted standard procedure when feasible. The spectrum of treatable patients is expanding to include many with serious emotional problems. This expansion places an added burden on staff and patient alike, as the stresses produced often cause significiant dysphoria even in the patient who is well integrated emotionally. Psychiatric morbidity must be expected and managed as well as circumstacnes will allow. As in most other human endeavors, patients who are likely to be successful are the fortunate few who can adapt to difficult and chronic stresses, undergo positive emotional experiences, and have a supportive family, satisfying work, solid identity, and comfortable self-esteem--in short, those with good mental health. The discussion speculates on personality or life-experience variables that might have prognostic usefulness.

Adaptation, Psychological↗
PubMed1975

Morphology of stored, rejuvenated human erythrocytes.

Whole blood was stored at 4 degrees C for 42 days. At 7-day intervals, starting with day 21, aliquots were rejuvenated with a phosphate, inosine, glucose pyruvate, and adenine solution. Samples were taken for ATP assays and red cell morphology (by SEM) both before and after rejuvenation. Rejuvenation elevated all ATP levels in excess of 100% of the starting values and significantly increased the percent of cells in the discoid shape as measured by their morphological index, showing that rejuvenation not only raises the cell's metabolic level but causes partial reversion to fresh cell morphology.

Adenine↗