The patterns of renal electrolyte excretion in the duck (Anas platyrhynchos) maintained on freshwater and on hypertonic saline.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
G L Fletcher
Publications and source records attributed to G L Fletcher.
Sequential changes in the adenosinetriphosphatase activity and the electrolyte excretory capacity of the nasal glands of the duck (Anas platyrhynchos) during the period of adaptation to hypertonic saline.
Explore the source record for details and available documents.
Adaptation, Physiological↗
Effects of gamma radiation on deoxyribonuclease I in the presence of high concentrations of second solutes.
Explore the source record for details and available documents.
Cellulose↗
A computerized scoring procedure for auditory brainstem response audiometry.
A weakness of auditory brainstem response (ABR) audiometry is the highly subjective manner in which the tracings are scored. This article describes a computerized scoring technique which was devised to reduce the subjective decision making in ABR test analysis. This correlation technique assumes only that the ABR, whatever its latency or waveform, will be highly consistent when the same stimulus conditions are repeated. This consistency should result in high correlations when two suprathreshold tracings are compared. In contrast, low correlations should occur when one of the tracings is a control containing no ABR. The extent of differences between correlations is used as the response criterion.
Adult↗
Development of an all-fish gene cassette for gene transfer in aquaculture.
To develop an all-fish gene cassette suitable for gene transfer in aquaculture, the antifreeze protein (AFP) gene promoter from the ocean pout (Macrozoarces americanus) was analyzed for its ability to direct exogenous gene expression both in vitro and in vivo. The ocean pout AFP (opAFP) gene promoter fused to the bacterial chloramphenicol acetyltransferase (CAT) was functionally analyzed in two fish cell lines and in Japanese medaka embryos. The opAFP gene promoter was active in these systems, as demonstrated by the transient expression of CAT activity. These results suggest that the opAFP gene promoter is useful for many other gene transfer experiments. To facilitate use of the opAFP gene promoter as a common and versatile vehicle for fish gene transfers, an expression vector, opAFP-V, was constructed by linking the 2.1-kb opAFP gene promoter, the 63-bp opAFP gene 5' untranslated sequence, and the 1.2-kb opAFP gene 3' sequence by two unique restriction sites, Bg/II and HpaI, respectively. Thus, genes of interest can be inserted into either the Bg/II site or the HpaI site depending on the length of their 5' untranslated sequence. The complete DNA sequence of opAFP-V was determined to facilitate future detailed analysis of integration and expression of the transgene.
Animals↗