Biomedical subjects
Publications and source records attributed to G L Colborn.
The surgical embryology and anatomy of the spleen are reported with emphasis given to ligaments, blood supply, and segmentation. The anatomic entities involved with splenic surgery are presented. Surgical applications are emphasized. Knowledge of splenic anatomy and technique, with efforts to save the organ if possible, is paramount for good results.
The embryology and surgical anatomy of the inguinal area is presented with emphasis on embryologic and anatomic entities related to surgery. We have presented the factors, such as patent processus vaginalis and defective posterior wall of the inguinal canal, that may be responsible for the genesis of congenital inguinofemoral herniation. These, together with impaired collagen synthesis and trauma, are responsible for the formation of the acquired inguinofemoral hernia. Still, we do not have all the answers for an ideal repair. Despite the latest successes in repair, we, to paraphrase Ritsos, are awaiting the triumphant return of Theseus.
The carpal tunnel syndrome is a compression of the median nerve within the carpal tunnel with very specific signs and symptoms. Anatomical dissection of 156 wrists is presented and then the authors compare their findings with the findings of other investigators. The purpose of this article is not to present results but rather to discuss the anatomic entities involved with the syndrome and to present the open treatment modality.
The carpal tunnel syndrome is a compression of the median nerve within the carpal tunnel with very specific signs and symptoms. Anatomical dissection of 156 wrists is presented and then the authors compare their findings with the findings of other investigators. The purpose of this article is not to present results but rather to discuss the anatomic entities involved with the syndrome and to present the open treatment modality.
The carpal tunnel syndrome is a compression of the median nerve within the carpal tunnel with very specific signs and symptoms. Anatomical dissection of 156 wrists is presented and then the authors compare their findings with the findings of other investigators. The purpose of this article is not to present results but rather to discuss the anatomic entities involved with the syndrome and to present the open treatment modality.
This study examines the incidence of the posterior gastric artery in a series of 75 adult cadavers. We clearly identified the posterior gastric artery in 36 (48%) cadavers as a 1- to 2-mm vessel arising from the cranial border of the splenic artery within 3 cm of the celiac trunk and coursing dorsally to the posterior parietal peritoneum where it forms a fold before supplying the upper part of the posterior gastric wall. Identifying this vessel is difficult during surgery that compromises vascular tributaries of the stomach, and the obscure course and high incidence of this vessel necessitates awareness that, in a 75-85% gastrectomy, the short gastric artery or branches of the left gastric artery should be preserved since the posterior gastric artery will be sacrificed in 13% of these cases.
The anatomy of the left renal vein, especially knowledge of collateral flow, is extremely important to the modern surgeon, since division of the left renal vein is common to many procedures. This study examines the left renal venous drainage system in 20 human cadavers for evidence of collateral flow and anomalies. Ten cadavers underwent dissection of the tissues surrounding the left renal vein, except for the left suprarenal and left gonadal veins, and 10 did not. Water and methylene blue were injected into the left renal vein to check for extravasation, and the veins were subsequently divided. There was no evidence of additional tributaries off the left renal vein as no extravasation occurred, and opening of the left gonadal and left suprarenal veins did not reveal any direct connections to the inferior vena cava. Thus, this experiment did not demonstrate evidence of a systemic collateral flow system draining the left kidney once the left renal vein was divided. Anomalies of the left renal venous drainage system occurred in six of 20 (30%) of cadavers, with one anomaly of the left renal vein itself (5%) manifested as a supernumerary left renal vein. The other anomalies included bifurcation of the gonadal vein, bifurcation of the suprarenal vein, the inferior phrenic vein draining into the left renal vein distal to the superior mesenteric artery, and the presence of a lumbar vein draining into the left renal vein in two cadavers. The lumbar veins may perhaps represent a normal variant.
Long before the Christian era, the duodenum was named and its function in controlling gastric emptying was conjectured. It received almost no further attention until the Eighteenth century when its relation to the bile and pancreatic ducts became know. The embryogenesis of the duodenum and the histological features of the organ are described as well as the gross movements that explain the mature relations of the duodenum to the surrounding structures. The "sphincters" of the duodenum are mentioned and evaluated.
The second part of this monograph on the duodenum describes the muscular and mucosal changes observed at the gastroduodenal junction. The structure of the duodenal wall and details of the intramural portion of the common bile and pancreatic ducts are described, together with the surgical anatomy of the four parts of the duodenum. The arterial supply, the venous and lymphatic drainage, and the innervations are described from the surgeon's point of view.
Duodenal pathology includes various developmental malformations and acquired lesions. This report provides brief descriptions of various congenital anomalies of the duodenum, including stenosis and atresia, annular pancreas and ectopic duodenal pancreatic tissue, megaduodenum, duodenal diverticula, preduodenal portal vein, and paraduodenal fossae. Acquired lesions, such as duodenal ulcer, tumors, vascular compression, and duodenal trauma are also described.
This is the last part of our efforts to present, if possible, the duodenum in toto as an anatomical and surgical entity. For all practical purposes, Part 4 is a short presentation of the anatomy involved in mobilization and exposure of the duodenum from a surgical standpoint with specific applications. A table with most of the anatomical complications of duodenal surgery is also included.
The roles of rabbit polymorphonuclear leukocytes (PMNL) and mononuclear cells (MC) for the regulation of ocular herpes simplex virus-1 (HSV-1) infection were studied. The antibody-dependent cellular cytotoxicity (ADCC) mediated by PMNL and MC from normal rabbit peripheral blood was assessed kinetically employing a specific 51Cr release assay. The HSV-1 infected primary cultures of rabbit corneal epithelium (PRCE) were used as the target cells to obtain a homologous assay system. The PRCE was prepared by an epithelium outgrowth technique and identified by electron microscopy. The expression of the surface HSV-1 antigens on PRCE was examined by indirect immunofluorescent staining; the cell population stained by fluorescein increased from 40% at 3 hr postinfection (PI) to 100% at 8 hr PI. To determine how early the cytotoxicity occurs, PRCE were infected with HSV-1 for 2 hrs. After 2 hrs, the ADCC was checked every 10 min for the first 40 min and then at 1, 2 and 4 hr of incubation. The cytotoxicity was apparent at 10 min postincubation and reached 46% by PMNL and 40% by MC at 4 hr postincubation (6 hr PI). Significant cytotoxic effect (26% by PMNL and 16% by MC) occurred as early as 3 hr PI. When the one-step growth cycle of HSV-1 was studied in the PRCE, HSV-1 had an eclipse period of 4 hr and a rise period of 8 hr. This suggests that rabbit PMNL and MC have the potential to eliminate the HSV-1 infected rabbit corneal epithelium before HSV matures in the cells.
Ocular HSV-1 shedding from latently infected rabbits was induced by iontophoresis of 0.01% epinephrine into the eye. Anodal Iontophoresis of epinephrine was performed at 0.8 mAmps for 8 min once a day for 3 consecutive days. Shedding was determined by the presence of HSV-1 in the preocular tear film obtained via eye swabs. Bilateral epinephrine iontophoresis performed on selected days during 220-280 days after inoculation resulted in HSV-1 shedding in 75% of the eyes (30/40) and 100% of the rabbits (20/20). Following the induction of ocular HSV-1 shedding, rabbits were killed and selected neural tissues were homogenized. Cell-free preparations were assayed for the presence of infectious virions using primary rabbit kidney cell monolayers. When the tissues were homogenized immediately after death, virus was detected in only one neural tissue, the trigeminal ganglia. However, when the tissues were incubated in vitro for 18-24 hours prior to the homogenization, infectious HSV-1 was recovered from homogenates of the trigeminal ganglion, superior cervical ganglion, the ophthalmic branch of the trigeminal nerve, and the root entry zone of the trigeminal nerve. A relationship was noted between the time of the last ocular shedding and recovery of infectious HSV from the tissue homogenates. Furthermore, a positive correlation in 11 eyes between the recovery of HSV-1 from the perocular tear film and HSV-1 recovery from one or more corresponding neural tissues was found. These results suggested that epinephrine iontophoresis to the cornea triggered an "alteration" in the state of the virus in the neural tissues of the latently infected rabbits and that the change can be related to the induced ocular shedding.
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The anatomy of the inguinal region is enigmatic and confusing. Among the many structures involved in hernial repair are the iliopubic tract, the transversus abdominis aponeurosis and the transversalis fascia, the transversalis crura and sling, and the inguinal canal. There is still much disagreement among surgeons and anatomists about the existence, structure, and function of these anatomic entities.