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Biomedical subjects

G Konat

Publications and source records attributed to G Konat.

50 records · Page 3Linked to original sources
PubMed1978

E rosette-forming lymphocytes in multiple sclerosis patients. Basic protein stimulation of rosette-forming cells.

"Total" and "active" T cells were enumerated using sheep red blood cell (SRBC) rosetting technique. The percentages of "total" and "active" T lymphocytes were not significantly different in multiple sclerosis patients as compared to other neurological disorders and/or healthy controls. However, the number of "avid" T cells binding more than three SRBC was significantly increased in MS patients. Active E rosette test has shown hypersensitization to myelin basic protein in 50% MS patients and 31% OND patients. All healthy controls studied, except one had negative response.

Adult↗
PubMed1977

Clinical, social and biochemical studies on Batten's syndrome, alias Spielmeyer-Vogot or Stengel's Syndrome.

The clinical and biochemical data on 13 patients with Batten's syndrome are described. Clinically the disease was characterized by progressive maental and somatic deterioration. Initially, vision loss was found between the ages 4 and 8 years. This was associated with 1 or 2 years of normal school attendance followed by attendance at a school for mentally retarded from the age of 8 to 11; then warding was established at a school for blind children and later on a hospital for epileptic patients when seizures and mental retardation made hospitalization necessary. Biochemically, an increased peroxidation rate was revealed in peripheral thrombocytes. This abnormality was associated with a significant decrease in peroxidase activity of leucocytes assayable with p-phenylenediamine, but not with Guajacol. The peroxidase defect seemed to concern an azide-resistant peroxidase. However, in serum the glutathione peroxidase was only found insignificantly decreased.

Blood Platelets↗
PubMed1977

Lymphocyte stimulation in multiple sclerosis patients untreated and treated with transfer factor. Long-term studies on the effect of different stimulants on myo-(2-3H)inositol incorporation into phosphatidylinositol of lymphocytes.

The lymphocytes from controls, untreated MS and MS patients treated with transfer factor were stimulated with PHA, PWM, PPD, LPS and MA and the increased incorporation of myoinositol into phosphatidylinositides was determined. As compared with controls, the untreated MS patients revealed significantly lower index of stimulation with PHA, LPS, PPD and MA. The response of lymphocytes from MS patients treated with transfer factor was intermediate. No correlation between disease progression and stimulation of inositol incorporation was observed. Analysis of variance showed that the myoinositol test is not applicable in long-term studies to distinguished single subjects, however it is useful to determine differences between MS patients and controls.

Adult↗
PubMed1975

Morphological changes of astrocyte-like cells induced by serum beta-lipoprotein in brain cell culture.

When added to brain cell cultures of newborn rats, serum and, in particular, its beta-lipoprotein fraction caused significant morphological transformations of astrocyte-like cells present in the culture. The changes were instantaneous as they appeared within 1 min after addition of beta-lipoprotein and they were characterized by swelling and loss of cellular processes of all astrocyte-like cells present. The lipoprotein effect was reversed after a period of about 7 h. Since the blood-brain and blood-spinal fluid barrier in multiple sclerosis is decreased towards serum macromolecules, i.e. the serum beta-lipoprotein, this protein may enter the central nervous system and thereby initiate a demyelinating process.

Animals↗
PubMedDate not supplied

Multi-sialo brain gangliosides are powerful stimulators of active E-rosetting lymphocytes from multiple sclerosis patients.

Peripheral blood T lymphocytes from all of 14 patients with clinically definite multiple sclerosis (MS) were significantly stimulated by MS brain gangliosides in the active rosetting of sheep erythrocytes. Fractionated mono- and disialogangliosides were devoid of any stimulating effect on MS lymphocytes whereas the trisialoganglioside GT1 and to a greater extent the tetrasialoganglioside GQ1b were fully effective at a dose as low as 2 x 10(-18) moles. Gangliosides extracted from MS brains or from MS brain myelin were far more effective than gangliosides derived from control human brains or from bovine and mouse brains, suggesting the importance of highly sialylated gangliosides occurring to a greater extent in MS brain as previously reported. Lymphocytes from only 3 out of 24 other neurological patients were stimulated by the slow migrating gangliosides in the same way, but none of 32 healthy subjects responded to these gangliosides in the active E-rosette test. Lymphocytes from 5 to 8 patients with unilateral optic neuritis reacted positively to brain gangliosides by rosette formation, several weeks before a similar reaction to myelin basic protein was evident. Our data are compatible with a release of gangliosides during demyelination or other CNS degenerative processes occurring in multiple sclerosis.

Adult↗
PubMedDate not supplied

The effect of serum from multiple sclerosis patients in remission on the incubated rat brain slices.

Addition of human serum to incubated rat cerebral slices induced increased generation of myelin-related, membranous fragments floating on 0.32 M sucrose. Sera from 20 healthy subjects and 19 patients with various neurological disorders were equally active in this respect. On the other hand, the myelin-degrading activity of sera from 20 multiple sclerosis patients in remission was found to be significantly elevated by about 50%. The present findings support the contention that the serum of multiple sclerosis patients possesses increased potency to induce myelin sheath alterations.

Adolescent↗