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Biomedical subjects

G Kolde

Publications and source records attributed to G Kolde.

102 records · Page 6Linked to original sources
PubMed1983

An ultrastructural study of epidermolytic leukoplakia.

A leukoplakic lesion of the lip showing the histologic features of epidermolytic hyperkeratosis above solar elastosis was investigated by electron microscopy. The ultrastructural alterations observed in the upper epidermal layers corresponded in the main with those of epidermolytic hyperkeratosis in other skin diseases. The keratinocytes showed irregularly formed tonofilaments, a marked intracellular edema, and premature cornification. In addition, there were discrete subcellular signs of premalignancy in the cells of the basal and suprabasal layers. These alterations suggest that the epidermolytic leukoplakia represents a rare histopathologic variant of actinic cheilitis.

Cheilitis↗
PubMed1982

Changes in neuronal arborizations induced by lesions of the ganglionic perineurium (Helix pomatia).

Lesions of the perineurium of the buccal ganglia of Helix pomatia have been found to elicit changes in neuronal arborizations. These changes were studied by light and electron microscopical techniques. In ganglionic regions of a damaged perineurium, the known arborizations of the identified neurones B1, B2 and B3 were characterized by additional and atypical fibres of small diameters and with multiple swellings. Damaged regions contained an increased number of neuronal fibres often showing vacuoles of great diameters. The changes observed 4-24 h after the damage of the perineurium are interpreted as neuronal sproutings.

Animals↗
PubMed1982

PO2-profiles in hippocampal slices of the guinea pig.

For a detailed analysis of the oxygen supply of hippocampal slices, tissue PO2 (Pt,O2) was recorded polarographically in the neural layers of thick and thin slice preparations from the guinea pig. The experiments showed that the Pt,O2-gradients were extremely steep in the outer zones of vital slices. In an air equilibrated salt solution the surface PO2 was reduced to less than 50% within ca. 25 micron. Minimum values were measured at a depth of ca. 150 micron. A rise of temperature lowered the oxygen supply in the deeper layers of the excised tissue. An elevation of the surface PO2 hardly improved Pt,O2 in the deep structures, because the O2-consumption of the hippocampal slices increased with rising PO2.

Animals↗
PubMed1982

Subsurface cisterns and lamellar bodies in the granule cells of the guinea-pig fascia dentata.

Subsurface cisterns (SSC's) and less frequent lamellar bodies (LB's) were identified in the granule cells of the guinea-pig fascia dentata. Both structures, composed of flattened or collapsed agranular cisterns, are continuous with the regular rough endoplasmic reticulum and occasionally connected with each other forming LB-SSC complexes. The SSC's are apposed to glia, synaptic boutons, and nerve cell processes as well as to neighbouring granule cells appearing here singly and in confronting pairs. The quantitative analysis of the various cisternal appositions compared to the distribution of the tissue components on the granule cell soma shows that the overwhelming majority of SSC's are related to glial cells.

Animals↗
PubMed1978

Ultrastructural morphometric investigations on normal human liver biopsies.

Normal liver biopsies of 14 volunteers were investigated ultrastructural morphometrically. Besides the determination of morphometric baseline data for comparative purposes particular emphasis was placed on the question, if morphometric evaluations of human livers yield reliable results in spite of the higher range of distribution than in experimental liver material. The results showed that the surface of the smooth endoplasmic reticulum of human liver is significantly greater than the surface of rough endoplasmic reticulum. Otherwise the morphometric data of the human liver tissue did not differ essentially to the values of the laboratory rat. The variance analytical consideration of the mean evaluated from the data of the 14 liver biopsies indicated that the standard error of nearly all proved parameters was less than 10% of the mean. The results suggest that ultrastructural morphometric evaluation of human liver biopsy samples yield reliable results provided the samples investigated are sufficiently extensive.

Animals↗
PubMed1978

[Ultrastructural-morphometric analysis of polyenylphosphatidylcholine [PPC] treated cholestatic rat liver (author's transl)].

Essential phospholipid PPC was administered to male rats over 24 and 72 hours after operation on bile duct ligation. The subsequent alterations were studied by ultrastructural morphometry and correlated with serum enzyme changes. By the results evidence is accrued that hepatocellular mitochondria are prevented by phospholipid treatment to develop alterations as seen after bile duct ligation alone. This occurred although serum GLDH activity was significantly increased. The smooth endoplasmic reticulum exhibited a normotrophic behavior after PPC treatment of animals in which bile duct ligation was done. The surface of the rough membranes was found increased at hours 24 and 72 after bile duct ligation. The bile canaliculi were markedly dilated. However, this alteration was not accompanied by a marked loss of microvilli. Administration of the phospholipid induced a numerical increase of hepatocellular nuclei in both sham-operated and cholestatic animals independantly from the bile duct ligation. It is suggested by the results that the administration of the phospholipid PPC is of value in influencing the fine structural hepatic alterations as seen in cholestatis.

Alanine Transaminase↗
PubMed1976

Effects of clofibrate (alpha-p-chlorophenoxyisobutyryl-ethyl-ester) on male rat liver.

In male rats, fed 0.5% clofibrate in their diet for 8 days and 21 days, the ultrastructural morphometric alterations of the hepatocytes were evaluated and compared with the biochemical data. The morphologic alterations of the microbodies were particularly related to the changes of the catalase activity of the liver homogenates. The results showd a marked hypertrophy of the liver and an increase in the volume of the individual hepatocyte. The numerical density and, even more pronounced, the volume density of the microbodies increased excessively during the treatment. The numerical density of the mitochondria decreased markedly after 21 days of administration. The surface of the rough endoplasmic reticulum showed a significant decrease, whereas the surface of the smooth endoplasmic reticulum showed a hypertrophy. The catalase activity of the liver homogenates increased 2-fold after 8 days and remained at this new steady-state after 21 days of treatment. The results suggest that the enzyme content of the microbodies changed after treatment with clofibrate, and support the suggestion that clofibrate may induce the synthesis of a yet unidentified peroxisomal protein.

Animals↗