Biomedical subjects

G Kleinhans

Publications and source records attributed to G Kleinhans.

22 records · Page 2Linked to original sources

PubMedDate not supplied

Flow cytometric analysis of the maldescended testis.

Flow cytometric measurements of the cellular DNA contents of 16 testicular biopsies of 15 patients with maldescended testes were performed. 2 patients were children. 11 patients of pubescent or adult age had no haploid germ cells in the tissue of their maldescended testis. One patient with a testis mobilis had about a normal percentage of haploid germ cells. In one patient a malignant tumor was detected unexpectedly by an aneuploid stem line at 2.2 c. Flow cytometric analysis of human testicular biopsy material is an easy and a fast way to quantify the function of spermatogenesis and detect malignancies.

Adolescent↗

PubMedDate not supplied

DNA-analysis of human testicular samples by cytofluorometry.

Germ cells in human testicular samples have been estimated by histology and quantitated by flow cytometry. There is an excellent relationship between the two techniques. These samples were classified histologically as "normal", "reduced number of germ cells" or "no germ cells". A definition of "normalcy" as determined by flow cytometry as having at least 38% of the cells in the haploid state, is proposed. Testicular samples from prepubertal boys with infiltrating leukemic cells often show an increase in the percentage of S-phase cells indicating the presence of tumor. These results indicate the value of flow cytometry in the identification of reproductive and neoplastic disorders.

Adolescent↗

PubMedDate not supplied

Preoperative renal tumor embolization. A useful procedure?

The medical reports of 113 patients operated upon for renal tumors were retrospectively reviewed to assess the value of preoperative embolization. Coils or ethanol were used preoperatively in 55 patients while 58 patients underwent nephrectomy without embolization. The tumors were staged according to the TNM and the Robson staging classification. T1 and T2 tumors as well as T3 and T4 tumors were grouped together for statistical evaluation. There was no significant difference in intraoperative blood loss, operation time and survival rate between patients undergoing preoperative embolization and those who had only nephrectomy. Duration of hospital stay was always longer in the preoperatively embolized group. Preoperative embolization of renal tumors neither improved the patients' outcome nor reduced the surgical risk. Robson's classification, stage III/IV, did not show any significant differences for embolized patients or for those nephrectomized without embolization.

Adult↗

PubMedDate not supplied

[Spermatogenesis in old age--nuclear DNA determination by flow cytophotometry in testicular samples of older men for the quantification of spermatogenesis].

The flow cytometric determination of the nuclear DNA content of testicular samples permits a fast and reliable quantification of spermatogenesis. 70 testicular samples from men over 50 years of age were analysed with this method. 60% of the samples showed a distinct reduction of spermatogenesis. Only three men showed a nonreduced spermatogenesis in both testes. Comparing the results of the measurements of both testes, in nearly half of the testes pairs a reduction of the germ cell number below two thirds of the better side was striking. In one patient, a Leydig cell tumor was detected by chance by an aneuploidy.

Aged↗