Biomedical subjects

G Klein

Publications and source records attributed to G Klein.

1,506 records · Page 84Linked to original sources

PubMedDate not supplied

[Coumarin therapy during antirheumatism therapy with sulindac].

Possible interactions between the new non-steroidal antirheumatic drug Sulindac and a coumarin derivative have been investigated. 20 subjects, who required anticoagulation therapy for a variety of medical conditions, were treated for 4 consecutive weeks with Sulindac at varying dosages (200-400 mg per day). Prothrombin time, Thrombotest and bleeding time were not significantly affected by the concurrent administration of Sulindac. It can be concluded that there is no clinically important interaction between phenprocoumon and the doses of sulindac that are considered effective in rheumatic disorders. There were some moderate side effects, only one drop out was registered because of gastro-intestinal bleeding (melaena).

4-Hydroxycoumarins↗

PubMedDate not supplied

[A clinical trial of sulindac in osteoarthrosis].

45 patients wih degenerative joint diseases (osteoarthrosis of the hip or knee, Heberden's nodes) were treated for 5 weeks in an open controlled trial with Sulindac, a new nonsteroidal antirheumatic drug (300-400 mg daily). The effectiveness of the drug was evaluated by the following criteria: changes of pain intensity with active movements, pain at rest, night pain, duration of morning stiffness and limitation of movement. Analysis of the global data showed in 64,4% excellent results. In 9.9% the results were good, in 17,8% moderate and there was no response in 6.6%. Other side effects (epigastric pain 8,9%, dizziness 2,2%) were not serious and reversible during the treatment with Sulindac.

Clinical Trials as Topic↗

PubMedDate not supplied

[Biorheological contribution to the problem of rheumatic joint diseases].

The rheological properties of synovial fluids from patients with different rheumatic diseases are discussed. Viscosities of 73 samples were determined and are compared to a standard of "healthy" human synovial fluid p. m. and to bovine synovia. Typical differences between "healthy", degenerative and inflammatory synovial fluids could be discerned. These differences can be characterized with several rheological parameters. The mechanism of lubrication in joints and the role of synovial fluid as a lubricant are discussed. Rheological properties of polymere solutions which could serve as a substitute for sick synovial fluids are described and compared to the properties of healthy synovia. Several possibilities for molecular changes which could account for the deterioration of synovial fluids in patients with different rheumatic diseases are discussed.

Arthritis↗

PubMedDate not supplied

[Nature and incidence of causes of death in chronic polyarthritis].

The study comprises 79 patients with rheumatoid arthritis and their death rate and causes of death in comparison to an age- and sex-matched conrol group. The patients had died in the years from 1952 till 1977 and an autopsy was done on all of them in the University Institute of Pathology. Infections turned out to be the most common cause of death (48%), second in rank were various cardiac diseases (20%) while the percentage of other causes of death never exceeded 10 per cent. Possible relations between infections and their importance as a cause of death are discussed.

Arthritis, Rheumatoid↗

PubMedDate not supplied

[Susceptibility for infections in patients with chronic polyarthritis].

The case histories and autopsy findings of 79 patients with rheumatoid arthritis (RA)--15 patients with and 64 patients without a history of long term corticoid treatment--who had died in the years 1952-1977 and who had been autopsied in the University Institute of Pathology of Graz were evaluated with respect to organic lesions caused by infections and were compared with an exactly matched control group. Both in the rheumatoid group material and in the 64 RA patients without steroid. long term treatment there was a highly significant accumulation of putrid bronchitis and pneumonia and a significant accumulation of pyelonephritis in comparison to the control group. As far as bronchiectases and pulmonary tuberculosis are concerned, there was an increased prevalence in the RA group, but this was beyond the range of statistical significance. Possible reasons that might account for the elevated susceptibility to infections in patients with RA are discussed.

Arthritis, Rheumatoid↗

PubMedDate not supplied

[Guidelines for synovial cytology].

Certain technical requirements have to be satisfied when synovial fluid is submitted for evaluation. This is to enable examination of the cell morphology and the polysaccharide and lipoid histochemistry of the cells and the dried liquid phase. The cell physiology should be considered when evaluating the cytogramme. Morphological and histochemical examination of the background indicate which biochemical estimations of the liquid phase are needed, and thus represent added diagnostic tools for evaluation of joint diseases. Cytolysis, cell involution and substrate condensation are discussed in detail.

Arthritis↗

PubMedDate not supplied

Fine needle aspiration of a renal cell carcinoma with eosinophilic globules. A case report.

Fine needle aspiration (FNA) of a renal cell carcinoma with hyaline eosinophilic globules is presented. The cytology of the tumor cells was characterized by intracytoplasmic, dense, well-circumscribed globules that had a bright magenta appearance on Diff-Quik staining. Tissue sections demonstrated the globules to be located predominantly within the cytoplasm of the clear cells of the carcinoma. Immunohistochemical and ultrastructural evaluation is discussed, as is the significance of this finding.

Adenoma↗

PubMedDate not supplied

[Abnormal specificities of immune alloantisera].

Sera from repeatedly immunized with allogenic lymphocytes contain IgG that can be fixed by syngenic tumour cells. When compared to the specific anti-H-2 immune response, the paradoxical tumour reactivity is often very high, suggesting an active immunization process. Selective absorptions have demonstrated that the majority of these "anomalous" IgG are directed against membrane components of tumour cells. The nature of the detected tumour-associated determinants is not well elucidated: they are not found on normal embryonnic or adult tissue, but are highly expressed by all in vitro murine malignant cell lines thus far tested, and to a lesser extent by the corresponding in vitro maintained tumours.

Animals↗

PubMedDate not supplied

[Monoclonal gammopathy in chronic polyarthritis].

Disturbances of immunoglobulin production have often been observed in preclinical multiple myeloma and have led to the question of a pattern of reactive formation of monoclonal gammopathies. In the documented rare case of coincidental Ig-G-myeloma and rheumatoid arthritis some possible similar pathogenetic mechanisms in both diseases are discussed.

Arthritis, Rheumatoid↗

PubMedDate not supplied

Assignment and ordering of twenty-three unique NotI-linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3.

Twenty-three unique NotI-linking clones, mainly isolated from the NRL1 library, were mapped and ordered by fluorescence in situ hybridization to human chromosome 3. All these clones were partially sequenced around the NotI sites and thus represent sequence-tagged sites. The EMBL nucleotide database was then searched with sequences from the NotI-linking clones using the FASTA program. This search revealed that the NRL-090 clone (at 3q24) contains the gene encoding human guanosine 5'-monophosphate synthetase (GMPS-PEN). To our knowledge, this is the first localization of this gene. Clone NL1-320 (at 3p21.3) contains a gene encoding arginine tRNA (97.3% identity in 73 bp), while clones NRL-063, NRL-097 and NRL-143 contain expressed sequences with unknown functions. Other clones displayed 60-85% similarities to cDNAs, CpG islands and other genes.

Base Sequence↗

PubMedDate not supplied

Werner syndrome: characterization of mutations in the WRN gene in an affected family.

Affected and unaffected members of a Caucasian family with Werner syndrome were analyzed for mutations in the recently described Werner syndrome (WRN) gene and for their relevance to phenotypic expression of chromosomal instability and x-ray hypersensitivity. Two distinct molecular alterations were documented in the family. Analysis of the genomic DNA revealed a single-base exchange from A to T at an intron-exon boundary in the otherwise strongly conserved 5' donor splice site. Consequently, exon 30 is spliced together with the intron. The ensuing structure could be confirmed by the presence and calculated size of the resulting RNA fragments. The patients, all compound heterozygotes, had a 1-bp deletion in the first third of the coding sequence in the other allele. The genotypes of the family members for these mutations were determined and consequences for the cellular phenotype of the otherwise unaffected heterozygotes are documented.

Adult↗

PubMedDate not supplied

Adult X-linked immunodeficiency (XID) mice, IGM-/- single knockout and IGM-/- CD8-/- double knockout mice do not clear polyomavirus infection.

The importance of antibodies for elimination of polyomavirus infection and the prevention of virus induced oncogenesis was studied, X-linked immunodeficiency (XID) mice, IgM-/- single knockout and IgMI-/- CD8-/- double knockout mice, all defective in antibody production, and normal control mice were infected with polyomavirus as adults. The mice were followed for presence of polyoma DNA with a polyoma specific polymerase chain reaction (PCR) over 6 weeks post infection (p.i.), a time point at which polyomavirus DNA is no longer detected in normal adult infected mice. As expected, virus DNA was not detected in normal mice 6 weeks p.i. In both IgM-/- single knockout and IgM-/- CD8-/- double knockout mice a disseminated infection was still observed by 6 weeks p.i. and the latter group of mice succumbed around two months p.i. In XID mice, only one third of the mice were still positive for viral DNA 6 weeks p.i. No polyomavirus induced tumors were observed in any of the mice during the 2-4 month observation period.

Animals↗