Biomedical subjects
G Johnsen
Publications and source records attributed to G Johnsen.
Intraoperative streptokinase in the treatment of superior mesenteric artery embolisation. Case report.
Two patients were treated with peroperative intra-arterial infusion of streptokinase in addition to embolectomy to improve blood perfusion and reduce the magnitude of bowel resection in embolism of the superior mesenteric artery. Perfusion of the bowel improved considerably during and after streptokinase infusion thereby avoiding major intestinal resection in both patients.
[Laser treatment of inoperable cancer of the esophagus and cardia].
The palliative effect of laser treatment for obstructing carcinomas of the oesophagus and gastric cardia was evaluated in 16 patients, between 59 and 81 years of age (median 73 years) and treated during the years 1986-89. All patients had severe dysphagia and none were able to eat solid food. They were judged inoperable because of advanced disease (9), reduced general condition, high age or concomitant disease (5). Two patients refused surgery. There was no procedure-related mortality. Complications were oesophageal perforation (1), bleeding (1) and development of fibrotic stricture (1). Ability to swallow was improved in all but one patient, and 13 became able to eat solid food. Seven patients needed repeated treatment 1-5 times at intervals of 3-10 weeks (median five weeks) between treatments. The results suggest that endoscopic laser treatment represents a good alternative in the palliative treatment of malignant obstructions in the oesophagus and gastric cardia.
Antigenic and allergenic determinants of ovalbumin--III. MHC Ia-binding peptide (OA 323-339) interacts with human and rabbit specific antibodies.
Three analogous peptides mimicking the known primary structure of ovalbumin (OA) in the region 323-339 (namely, OA 323-339, OA 323-338 and OA 324-336) were manually synthesized by the Merrifield solid phase technique. The synthetic preparations were purified by gel filtration and ion exchange high performance liquid chromatographies. The sequence linearities were deduced from the amino acid composition prior to each stage of coupling and the amino acid sequence determination on the ultimate chain. OA 323-339 was conjugated to BSA, using carbodiimide activation of the carrier protein and the conjugated compound was further used for production of antibodies in rabbit. The results showed that the region OA 323-339 was immunogenic; it could give immunoprecipitates with rabbit anti-OA 323-339-BSA in crossed immunoelectrophoresis (CIE). It similarly could bind human specific IgE from serum pools from patients allergic to egg in crossed radio immunoelectrophoresis (CRIE). Quantitative precipitation inhibition and specific IgE inhibition were used for confirming the antigenic and allergenic activities of this region. The results led us to conclude that the region 323-339 of the OA molecule encompassed an allergenic and antigenic epitope which were recognized by human and rabbit B-cells.