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Biomedical subjects

G Johansson

Publications and source records attributed to G Johansson.

At least 361 records · Page 20Linked to original sources
PubMed1979

Interaction of human serum albumin with fatty acids. Role of anionic group studied by affinity partition.

The interaction of human serum albumin with fatty acids has been determined using the method of affinity partitioning in aqueous biphasic systems containing dextran, poly(ethylene glycol) and esters of dicarboxylic acids with poly(ethylene glycol). The difference in the partition of albumin in phase systems with and without the poly(ethylene glycol)-bound fatty acid group provides a measure of the interaction of fatty acids with the protein. The relative contribution of the polar and non-polar interaction to the binding of fatty acids to albumin has been estimated by comparing the present data with that obtained earlier using poly(ethylene glycol)-bound straight chain aliphatic hydrocarbons. In both cases, the aliphatic chain should contain a minimum of 8 carbon atoms to affect the partition of albumin and that the maximum effect is obtained with chains containing 16 carbon atoms. The effect of the polymer-bound fatty acid group is higher than the corresponding hydrocarbon only when the number of carbon atoms in it exceeds 12. The relative effect of polymer-bound 16-carbon chains, with and without a carboxyl group in the terminal position is independent of pH in the range 5--9.

Fatty Acids↗
PubMed1979

Effects of therapy with bendroflumethiazide in patients with recurrent renal calcium stones.

Forty-four patients with recurrent formation of calcium-containing renal stones were treated with bendroflumethiazide for at least 2 years. Prior to treatment each patient had formed, on average, one stone per year for 8 years; during treatment only 4 patients formed new stones. A reduction in urinary calcium excretion was seen in almost all patients irrespective of their initial urinary calcium level. The apparent clinical benefit was not related to pre-treatment urinary electrolyte levels. Side effects were slight: one patient developed symptomatic hyperuricaemia and in one case sustained hypercalcaemia was found. Long-term treatment with thiazides appears to be a safe and effective method for the prevention of recurrent calcium stones.

Bendroflumethiazide↗
PubMed1979

Magnesium metabolism in healthy subjects.

Magnesium metabolism was studied in 106 apparently healthy subjects aged between 15 and 80 years. The serum magnesium values had a gaussian distribution with a narrow range and with no age or sex dependence. The basal, urinary magnesium excretion was greater in men than in women at all ages and decreased with age. The magnesium and calcium excretions were closely correlated in both sexes. The fractional, gastrointestinal absorption of magnesium, assessed by a newly developed technique using the radionuclide 28Mg, compared favourably with that in earlier balance and tracer studies. The intracellular magnesium content, estimated in muscle specimens obtained by percutaneous biopsies, was not correlated to age or sex. A magnesium-loading test was performed, and the normal range for magnesium retention was calculated. No correlation was demonstrated between different magnesium parameters within the normal range in these subjects.

Adolescent↗
PubMed1979

Indications of pH-induced conformational changes in phosphofructokinase from baker's yeast.

The partition of phosphorfructokinase (EC 2.7.1.11) from baker's yeast between the liquid phases of an aqueous biphasic systeem changes drastically in the pH interval 7-8, in contrast to other proteins. This abnormal behaviour is correlated to changes in sedimentation coefficient and binding capacity of the enzyme in this pH region. Since the molecular weight of phosphofructokinase does not change, these findings must reflect conformational changes in the enzyme molecule.

Hydrogen-Ion Concentration↗
PubMed1978

Comparison of two aqueous biphasic systems used for the partition of biological material.

Two biphasic systems, both consisting of water, dextran and poly(ethylene glycol) but differing in the molecular weight of the latter polymer, Mr = 35 000 (systems A) and 6000 (systems B), have been studied. The phase diagrams for the two systems at 20 degrees are compared. The partition of proteins influenced by salts and the viscosity of the phases are direct functions of the difference in composition between the upper and the lower phase as measured by the lengths of the tie-lines found for the systems in the phase diagram. The solubility of gamma-globulin is 1.5-2 times larger in an A system than in a B system with corresponding tie-line. In both systems, poly(ethylene glycol) palmitate has less effect on the partition of serum albumin than would be predicted from the distribution of poly(ethylene glycol) and reflects the non-ideality of simple equilibrium models.

Animals↗
PubMed1978

HLA and kidney stone disease.

Normocalcemic patients with recurring kidney stones were HLA typed. 54 patients with an idiopathic high urinary excretion of calcium but without signs of renal tubular defect showed a B27 phenotype frequency which was more than twice that of the controls and a CW1 frequency that was doubled. After correction for the number of tested HLA specificities the increase was not significant. 35 patients with renal tubular acidosis and 16 patients with no metabolic diagnosis had no deviant HLA antigen distribution.

Acidosis, Renal Tubular↗