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Biomedical subjects

G Jacobi

Publications and source records attributed to G Jacobi.

At least 37 records · Page 2Linked to original sources
PubMed1988

Expression of the HSV-2 ribonucleotide reductase subunits in adenovirus vectors or stably transformed cells: restoration of enzymatic activity by reassociation of enzyme subunits in the absence of other HSV proteins.

We have cloned the large subunit (RR1) of the HSV-2 ribonucleotide reductase into a helper-independent adenovirus 5 vector under control of the viral major late promoter. Infection of 293 cells with the AdRed-1 recombinant virus resulted in the expression of the HSV-2 RR1 protein. We have also produced cells which constitutively express the small (RR2) subunit of the HSV-2 enzyme by transfecting 293 cells with a plasmid encoding this protein and the neo resistance marker (pSV2neo-RR2). Infection of the A439-14 producer cells with AdRed-1 resulted in the expression of enzymatically active HSV-2 ribonucleotide reductase. HSV-2 reductase activity could also be detected upon mixing of extracts from cells expressing either subunit. Our results indicate that the HSV-2 holoenzyme can be reconstituted in vivo and in vitro and that no HSV-2 proteins, beyond the enzyme subunits, are required for the formation and activity of the viral reductase.

Adenoviridae↗
PubMed1988

[Van der Woude syndrome in combination with ring chromosome 18].

7-month-old girl with a combination of van der Woude syndrome and ring chromosome 18 showed the following malformations: medial palate cleft, fistulas of the lower lips, hypertelorism, epicanthus, antimongoloid position of the eyelids, a small hymenal appendix, plantar placing of the third toe, atrial septum defect, stenosis of the auditory canal. The combination of fistulas of the lower lip and cleft lip or palate already established the diagnosis of Van der Woude Syndrome. Because of the additional malformations observed a chromosome analysis was performed which demonstrated a ring chromosome 18. The phenotypic features of ring-chromosome 18, a 18p-/18q- syndrome, vary according to the extend of deletion of the short or long arm. However, all patients show craniofacial dysmorphism, intellectual and developmental retardation, defective hearing, speech disorder, and more than 50% have stenosis or atresia of the auditory canal.

Abnormalities, Multiple↗
PubMed1986

[Damage patterns in severe child abuse with and without fatal sequelae].

In 62 battered children with involvement of the central nervous system clinically 3 patterns of impact to the skull, brain and its coverings could be distinguished: In 22 babies (mean age 6 months) the brain was damaged mainly by violent shaking. Many of those infants were in shock and epileptic status on admission. Retinal, subarachnoidal, and later subdural bleedings were recognizable. At first, all patients survived, but later 3 of them died in a vegetative state. Retinal bleedings are prompted by subarachnoidal hemorrhage due to shearing of bridging veins and by compression of the chest which immediately is followed by raise of venous pressure in the upper half of the body and by arteriospasms, leading to endothelial damage and increase of vascular permeability (Purtscher's disease). Both mechanisms result in severe retinal hemorrhage which might intrude into the vitreous body (Terson-syndrome). The consequences of violent shaking for the child might be disastrous mental retardation, microcephaly, spasticity, and epilepsies. The child might turn blind on one or both eyes; the visual failure is due to retinal scar formation, retinal detachment and fibrous organisation within the vitreous body on the one hand, due to raised intracranial pressure on the other hand, adding further damage to the visual pathway. In 19 children whose mean age was 13 months massive impact on the skull resulted in major brain damage: acute subdural hematoma, contusional bleedings, compound, diastatic or impression fractures. Ten of them died immediately or were picked up dead from home by different emergency services.(ABSTRACT TRUNCATED AT 250 WORDS)

Battered Child Syndrome↗
PubMed1986

Cranial nerve damage after paediatric head trauma: a long-term follow-up study of 741 cases.

A report is given on transient and permanent (6 months) impairment of cranial nerves after paediatric head injuries (N: 741). There is a link between severity of the injury, fractures on the base of the skull, its foramina and channels, and the frequency of cranial nerve involvement. One should try to establish whether a posttraumatic dysfunction of the cranial nerves is primary or secondary in nature, i.e. due to raised intracranial pressure or haemorrhage. In children after head injuries often the cranial nerves of the oculomotor system are affected (20.2%/7.0%)--transient (permanent), followed by optic atrophy (4.88%), lesion of the trigeminal nerve (4.2%/2.2%), and the facial nerve lower motor type (4.1%/1.7%). Loss of hearing (3.3%/1.2%) and of smell (3.2%/1.2%) are less frequent in children than in adults.

Child↗
PubMed1984

[Pattern ERG and VECP following optic neuritis in childhood].

Pattern ERGs and pattern VECPs were recorded in 12 patients who had suffered from optic neuritis in childhood. When each individual's eyes were compared, the eye with the longer P100-latency in pattern VECP was found to have the smaller amplitude in pattern ERG. The latency differences correlated with the amplitude differences. These findings confirm that after optic neuritis the amplitude of pattern ERG is reduced.

Adolescent↗
PubMed1984

Psoas-hitch ureteroneocystostomy: experience with 181 cases.

The operative technique, indications and results of the psoas-hitch ureteroneocystostomy in 181 patients are reported. The principles of this procedure are as follows. Dissection of the ureter under direct vision, fixation of the mobilized bladder to the psoas muscle, longitudinal opening of the bladder up to the point of fixation, implantation of the ureter in an immobile bladder portion using a long submucosal tunnel. The original course of the ureter is maintained and is identical to the axis of the fixed bladder part. The broad spectrum of indications includes the repair of distal ureteral defects due to iatrogenic lesion, radiotherapy or inflammation, correction of congenital ureteral anomalies as well as therapy of reflux in adulthood and complicated reflux in children. Especially in cases in which previous ureteroneocystostomy has failed, the psoas-hitch plasty is the method of choice. Contraindications are neurogenic bladder dysfunction and a highly reduced bladder capacity or the impossibility of bladder mobilization because of a 'frozen pelvis'. The success rate of psoas-hitch ureteroneocystostomy performed in 181 cases was 96.7%. The preconditions for a successful ureteroneocystostomy are: tension-free anastomosis between ureter and bladder; antireflux implantation of the ureter through a long submucosal tunnel, and a straight course of the implanted ureter without kinking at the point of entrance into the bladder.

Adult↗
PubMed1981

[Superselective embolisation in the urogenital tract with tissue adhesives (author's transl)].

The tissue adhesive butyl-2-cyanoacrylate mixed with lipiodol and tantulum powder is an excellent agent for superselective catheter embolisation of small vessels. The mixture is low viscous and can be injected through fine catheters. Occlusion is independent of blood clotting and is permanent. A coaxial catheter technique is recommended or, in cases of difficulty, a ballon catheter can be floated in. Super-selective embolisation was used in 15 patients, in seven for the kidney in eight for the pelvis. Indications were bleeding from the kidney, partial defunctioning of the kidney, tumours in single kidneys and bleeding from carcinomas in the bladder, prostate or cervix.

Adenocarcinoma↗
PubMed1981

[Complicated migraine in children (author's transl)].

444 children and adolescents (266 boys, 178 girls) were examined because of migraine at the Department of Pediatric Neurology of the University Children's Hospital, Frankfurt/Main between 1967 and 1980. 378 of these had common and/or classical migraine, 66 complicated migraine. Family history, sex and age at onset in these two groups are compared. Based on this material (4 cases of ophthalmoplegic, 49 of hemiplegic and 13 of basilar artery migraine) clinical features, EEG findings angiographic and CAT-findings of these different forms of complicated migraine are discussed and demonstrated by typical case reports. The importance of basilar artery migraine even in childhood and the mainly poor prognosis of its special type of early onset- "alternating hemiplegia" - are stressed.

Adolescent↗
PubMed1979

[Double urologic tumors: reduced immunocompetence?].

55 patients with primary multiple neoplasias underwent immunostaging with determination of the immunoglobulins, B- and T-lymphocyte counting, H3-lymphocyte stimulation (ConA, PWM, PHA) and DNCB-epidermal test. There were no significant differences between the PMN-group and a group of patients with solitary tumors, whereas both cancer patient groups revealed a diminished immunocompetence compared with a non-malignant control group of patients.

Adult↗
PubMed1978

[Microrheological studies in healthy subjects. Effect of cigarette smoke and oral contraceptives on erythrocyte flexibility].

Erythrocyte flexibility was tested in 29 healthy subjects, using whole-blood filtration through membrane filters with 5 micron pores, special attention being given to cigarette smoking and oral contraceptives as risk factors. Red cell flexibility was significantly reduced in male smokers compared with non-smokers (P is less than 0.0001), and in all male and female smokers (P is less than 0.05) An influence of oral contraceptives to erythrocyte flexibility could not be found. Reduced erythrocyte flexibility in smokers as a factor contributing to an abnormal microcirculation and to the development of arterial occlusive disease is discussed.

Adolescent↗