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G J Rose

Publications and source records attributed to G J Rose.

At least 37 records · Page 2Linked to original sources
PubMed1988

Anatomical and functional organization of the prepacemaker nucleus in gymnotiform electric fish: the accommodation of two behaviors in one nucleus.

The diencephalic prepacemaker nucleus (PPn) of gymnotiform electric fish projects to the medullary pacemaker nucleus and modulates its regular firing frequency. Each firing of the pacemaker, in turn, drives an electric organ discharge (EOD). Two types of PPn neurons were retrogradely labeled from the pacemaker with HRP in Eigenmannia and Apteronotus. In both species, smaller ovoidal cells were found in the dorsomedial part of the PPn (PPn-G), and larger multipolar cells were found in the ventrolateral part of the PPn (PPn-C). This morphological distinction between the two subnuclei in the PPn was paralleled by a functional dichotomy. Microiontophoresis of L-glutamate in the PPn-G of both species elicited slow and gradual accelerations of EOD frequency characterized by a time constant on the order of seconds. The elicited frequency modulations were similar to those observed during the jamming avoidance response and during courtship. Glutamate stimulation of the PPn-C, in contrast, produced fast and abrupt frequency modulations characterized by a time constant on the order of milliseconds. These abrupt modulations resembled "chirps" observed during courtship and aggression. Similar behavior was produced by intracellular current injection into a PPn-C neuron of Apteronotus, and intracellular labeling of this neuron with Lucifer Yellow revealed a multipolar PPn-C neuron similar to those retrogradely labeled with HRP.

Action Potentials↗
PubMed1988

'Recognition units' at the top of a neuronal hierarchy? Prepacemaker neurons in Eigenmannia code the sign of frequency differences unambiguously.

The electric fish, Eigenmannia, is able to discriminate the sign of the frequency difference, Df, between a neighbor's electric organ discharges (EODs) and its own. The fish lowers its EOD frequency for positive Dfs and raises its frequency for negative Dfs to minimize jamming of its electrolocation ability by a neighbor's EODs of similar frequency. This jamming avoidance response (JAR) is controlled by a group of 'sign-selective' neurons in the prepacemaker nucleus (PPN) that is located at the boundary of the midbrain and the diencephalon (Fig. 1). Extracellular recordings from a total of 35 neurons revealed a great similarity between behavioral and neuronal response properties: 1. All neurons fired vigorously for negative Dfs and were almost silent for positive Dfs, regardless of the orientation of the jamming stimulus, and thus discriminated the sign of Df unambiguously (Fig. 2). 2. In accordance with behavioral observations, individual neurons failed to discriminate the sign of Df when the jamming stimulus had the same field geometry as the signal mimicking the animal's own EOD (Fig. 3). 3. Df magnitudes which evoke strongest JARs, usually 4 to 8 Hz, also induced most vigorous responses in sign-selective neurons (Fig. 5). 4. Behavioral and neuronal thresholds for the detection of small jamming signals were similar. Threshold for sign selectivity was reached when the amplitude ratio of the jamming signal to the EOD mimic, measured near the head surface, was 0.001. This value corresponds to a maximal temporal disparity (a necessary cue for performing a correct JAR) of 1 to 2 microseconds for signals received by the two sides of the body in a transverse jamming field (Fig. 7). 5. The effects of two jamming fields, offered orthogonally to each other, may interact nonlinearly at the behavioral as well as at the neuronal level. A positive Df presented in one field may suppress behavioral and neuronal responses to modulations of the sign of Df in the other field (Fig. 8c).

Animals↗
PubMed1987

The optic tectum of the gymnotiform electric fish, Eigenmannia: labeling of physiologically identified cells.

A total of 47 tectal neurons of the weakly electric fish, Eigenmannia, were studied physiologically and labelled by intracellular injection of Lucifer Yellow. With the exception of two cell types, all cells could be classified in accordance with the Golgi studies of Sas and Maler. The dominant stimulus modality of neurons was correlated with their laminar location. Neurons of the stratum opticum only responded to visual stimuli, such as modulations of the light level or the motion of an object. They showed, however, no directional preferences for motion. Neurons of the stratum griseum centrale were predominantly driven by electrosensory stimuli, most often those associated with the movement of an object, and generally were very sensitive to the direction of motion. Integration of different sensory modalities was found in neurons with dendrites invading laminae with different sensory inputs. In addition, small axons of interneurons appear to relay information across laminae. Large multipolar neurons in the deep tectum responded to the motion of objects, often preferring a particular direction of motion. Some of these large multipolar neurons of the deep tectum also discriminated the sign of the frequency difference between a mimic of a neighbor's sinusoidal electric organ discharge and the animal's own signal. These neurons are potential candidates for the control of the jamming avoidance response. These neurons were morphologically indistinguishable from large multipolar neurons of the deep tectum that either responded to moving objects or to acoustical stimuli. Individual large cells of the deep tectum project to various targets (Fig. 1) and probably contribute to the control of different behavioral responses. This suggests that the nature of such responses would then depend upon the constitution of sets of neurons recruited by a given stimulus situation, and the role of individual tectal neurons would neither be particularly specific nor very significant.

Animals↗
PubMed1986

A time-comparison circuit in the electric fish midbrain. I. Behavior and physiology.

Behavioral experiments show that the weakly electric fish, Eigenmannia, detects differences in timing as small as 400 nsec between electric signals from different parts of its body surface. The neural basis of this remarkable temporal resolution was investigated by recording from elements of the phase-coding system, a chain of electrotonically connected neurons devoted to the processing of temporal information. Each element of this system fires a single action potential for every cycle of the electric signal (either the fish's own electric organ discharge or a sinusoidal signal of similar frequency). For phase-coding primary afferents and midbrain neurons, the temporal resolution was determined by measuring each unit's capacity to lock its spike to a particular phase of the stimulus cycle. The jitter of a neuron's response (measured as the standard deviation of the timing of the spikes with respect to the stimulus) decreases from the level of the primary afferent (mean = 30 microsec) to the midbrain torus (mean = 11 microsec); these results can be correlated with morphological measures of convergence. The temporal resolution of single neurons is still inferior to that displayed at the behavioral level.

Action Potentials↗
PubMed1985

Species specificity and temperature dependency of temporal processing by the auditory midbrain of two species of treefrogs.

The mating (advertisement) calls of two sibling species of gray treefrogs, Hyla versicolor and Hyla chrysoscelis, are spectrally identical but differ in trill rate; being higher for H. chrysoscelis. Single-unit recordings were made from the torus semicircularis of both species to investigate the neural mechanisms by which this species-specific temporal feature is analyzed. Using sinusoidally amplitude-modulated (AM) white noise as a stimulus, the temporal selectivity of these midbrain auditory neurons could be described by five response categories: 'AM nonselective' (34%); 'AM high-pass' (7%); 'AM low-pass' (6%); 'AM band-suppression' (12%); 'AM tuned' (40%). The distributions of temporal tuning values (i.e., modulation rate at which each AM-tuned unit responds maximally) are broad; in both species, neurons were found which were tuned to modulation rates greater than those found in their advertisement calls. Nevertheless, the temporal tuning values for H. versicolor (median = 25 Hz) were significantly lower than those for H. chrysoscelis (median = 32.5 Hz). The temporal selectivities of AM band-suppression neurons were found to be temperature dependent. The modulation rate at which a response minimum was observed shifted to higher values as the temperature was elevated. These results extend our earlier findings of temperature-dependent temporal selectivity in the gray treefrog. The selectivity of band-suppression and AM-tuned neurons to various rates of amplitude modulation was largely, but not completely, independent of whether sinusoidal or natural forms of AM were used.

Animals↗
PubMed1985

Sensitivity to amplitude modulated sounds in the anuran auditory nervous system.

Auditory responses were recorded from single units in the eighth nerve and in the midbrain torus semicircularis of the leopard frog (Rana pipiens). Acoustic stimuli included sinusoidally amplitude-modulated (AM) tones and noise, as well as pure tones. Mean spike rates were measured at various rates of AM, and the degree to which a unit's spikes were restricted to a particular phase of the modulation cycle was described by a synchronization coefficient. The firing rate of eighth-nerve fibers was largely independent of the rate of AM over the modulation range 10 to 150 Hz. Further, the general shape of the spike rate vs. AM-rate function was invariant with either depth of modulation or sound-pressure level (SPL). Although virtually all eighth-nerve fibers exhibited significant synchronization to the envelope of AM, the shape of the synchronization function depended on the unit's best-excitatory frequency (BEF). Fibers with highest BEF's, presumed to innervate the basilar papilla, generally showed greater synchronization as the AM rate was increased (up to 100-150 Hz). Fibers tuned to the low-and midfrequency region, which innervate the amphibian papilla, exhibited low-pass synchronization characteristics. As the depth of modulation was reduced, the degree of synchronization of eighth-nerve fibers decreased. For a given depth of modulation an increase in sound level tended to decrease the degree of synchronization, but significant synchronization could still be observed at stimulus intensities at least 65 dB above threshold. On the basis of the spike rate vs. AM-rate functions, the temporal selectivity of single cells in the torus could be characterized by five response types: AM nonselective (spike rate was largely independent of the AM rate); AM high-pass (activity increased as the AM rate was increased); AM low-pass (response was greatest for slow AM rates and decreased at high rates); AM band-suppression (these neurons responded well to low and high AM rates, but responded weakly to intermediate rates); and AM-tuned (spike rate was greatest over a narrow range of modulation rates). In these measurements the depth of modulation was held constant at 100%. The five response categories are not discrete, but rather reflect representative examples along a continuum with regard to temporal selectivity. The temporal selectivity exhibited by toral units in their firing rates was not evident in their AM-synchronization functions.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1984

The anuran superficial reticular nucleus: evidence of homology with nuclei of the lateral lemniscus.

The superficial reticular nucleus (SR) of ranid frogs is part of a lateral cell column extending from the isthmus to the rostral tegmentum. The caudal part of this nucleus receives input from lower brainstem auditory nuclei and projects bilaterally to the torus semicircularis. On the basis of its position, connections, and sensitivity to acoustic stimuli, the caudal SR appears to be homologous to all or part of the mammalian nuclei of the lateral lemniscus.

Animals↗
PubMed1979

A characteristic effect of hallucinogens on investigatory responding in rats.

The disruption of the temporal distribution of investigatory responses by rats in a novel hole-board following lysergic acid diethylamide-25 (LSD), as described in a companion paper (Geyer and Light, 1979), was found to be a characteristic effect of a variety of hallucinogens. Similar effects were produced by indoleamine hallucinogens, such as LSD, N,N-dimethyltryptamine, and psilocin, and by phenylethylamine hallucinogens, such as mescaline or 2,5-dimethoxy-4-methylamphetamine (DOM). Congeners of DOM that are inactive in humans had no significant effects. Furthermore, of a variety of other psychoactive drugs tested, only apomorphine produced an effect similar to that of the hallucinogens. These results suggest that a simple behavioral measure of exploration in a hole-board may provide a useful animal model with which to examine the common effects of hallucinogens.

DOM 2,5-Dimethoxy-4-Methylamphetamine↗
PubMed1979

Mescaline increases startle responding equally in normal and raphe-lesioned rats.

To test the possible involvement of serotonin-containing cells of the midbrain in mediating the effects of mescaline on startle responding, electrolytic lesions were made in either the dorsal or median raphe nucleus in rats. Decreases in either striatal or hippocampal tryptophan hydroxylase activity confirmed the effectiveness of the lesions. One week later, startle was measured in response to 30 air-puff stimuli for each rat. Median, but not dorsal, raphe lesions increased startle magnitudes throughout the test session. The following day each group was divided into matched halves and were given 60 trials, 30 minutes after intraperitoneal injection of either saline or 10 mg/kg mescaline. Despite the large differences in baseline startle among the groups, mescaline produced comparable 25% increases in startle magnitudes in both sham- and raphe-lesioned animals. This result fails to support the hypothesis that increased startle responding produced by mescaline is mediated by the midbrain raphe nuclei.

Animals↗
PubMed1978

The effects of lysergic acid diethylamide and mescaline-derived hallucinogens on sensory-integrative function: tactile startle.

Tactile startle responding by male Sprague-Dawley rats given 60 presentations of air-puff stimuli (37.5 psi) was measured after the intraperitoneal administration of graded doses of hallucinogens and other psychoactive drugs. Among the drugs tested were the indoleamine-derived compounds, lysergic acid diethylamide (LSD), N,N-dimethyltryptamine and psilocin, and the phenylethylamine-derived compounds, mescaline, 2,5-dimethoxy-4-methylamphetamine and a series of active and inactive congeners of 2,5-dimethoxy-4-methylamphetamine. All of the active phenylethylamines increased startle response magnitudes throughout the test session. This pattern of augmented startle suggests that these drugs increase reactivity. However, none of the indoleamine hallucinogens increased startle responding. Of the nonhallucinogenic drugs tested, only apomorphine increased startle responding, while clonidine significantly decreased it, and amphetamine, chlorimipramine, scopolamine and methysergide had no effect. In additional studies with LSD, it was found that LSD increased the response to only the first stimulus when more intense air-puffs were used (50 psi). Furthermore, when the number of stimuli was increased from 60 to 240 (1 hr) so that appreciable habituation was evident in controls, LSD impaired this habituation. Whereas the response magnitudes of the control group decreased by 70% across the session, the responses of LSD-treated rats decreased by only 32%. These results suggest that LSD and phenylethylamine-derived hallucinogens may differ in their effects on tactile startle responding.

Animals↗