The effect of endotoxin on the synthesis of connective tissue matrix components by pulp fibroblasts in vitro.
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Biomedical subjects
Publications and source records attributed to G J Pinero.
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Specimens of mandibular condyle from human cadavers were employed for scanning electron microscopic and radiographic observations of the articulating surface and underlying bone. Smooth articular surface supported by smooth bone was most frequently observed. Craters and depressions in the articular surface were associated with resorption of underlying bone. Lateral radiographs proved to be of limited value in detecting defects of this type.
1. The generally poor state of soft tissue preservation that complicates observations by gross dissection and light microscopy posed no hindrance to scanning electron microscopic (SEM) studies of bone surface, because all of the soft tissues are chemically removed. 2. Specific and identifiable surface patterns representing locations of periosteal and fibrous muscle attachments can be readily observed in cadaver tissue with the SEM. 3. SEM observations confirmed earlier literature concerning the extent of periosteal and intraosseus attachments of the masseter muscle. The masseter muscle attached periosteally on the lateral surface of the mandible and along the inferior border. Just above the inferior border, a ridge of intraosseous attachment was noted. 4. The correlation of SEM observation of bone surface patterns with gross dissection and light microscopy demonstrated that SEM can be useful in determining the extent of muscle attachments in cadaver tissue even when the soft tissue is poorly preserved.
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Phatocytosis by a human gingival epithelial-like cell line in tissue culture was studied using latex beads as a marker. Electron microscopic and chemical analyses revealed that the cells ingested the beads in a quantitative and reproducible manner. This phagocytosis system is potentially useful for studying the effects of dental plaque on cell metabolism.
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