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G J Mitchell

Publications and source records attributed to G J Mitchell.

At least 73 records · Page 4Linked to original sources
PubMed1990

A nurse responds.

Explore the source record for details and available documents.

Human Development↗
PubMed1989

Delayed return of gastric emptying after gastroenterostomy.

A retrospective study of patients having gastroenterostomies was undertaken to identify predictive factors for the development of postoperative delayed return of gastric emptying (DRGE). A total of 322 consecutive patients underwent 324 gastroenterostomies; 35 experienced delayed return of gastric emptying. Regression analysis demonstrated preoperative obstruction to be the most significant factor (P less than 0.001). Vagotomy was not an independent variable. Age, sex, size of stoma, anastomotic technique, albumin and experience of the operator were not significant factors. Gastroenterostomy in the presence of 'gastric atony' was likely to produce DRGE. In all, 86 per cent of cases resolved spontaneously. Only one case of DRGE was found to have a mechanical cause. Most patients were supported by parenteral nutrition but, with a knowledge of the identified risk factors, more thought could be given to establishing a route for jejunal feeding at the time of surgery.

Adolescent↗
PubMed1978

Structure of recombinant plasmids containing synthetic human foetal globin gene sequences.

In vitro synthesized duplex DNA complementary to human foetal globin messenger RNA was integrated into bacterial plasmids and amplified by transformation of Escherichia coli. Recombinants carrying globin DNA were identified by hybridization of foetal globin messenger RNA to bacterial DNA in situ and by liquid hybridization of purified plasmids to specific globin complementary DNA probes. Heteroduplex mapping revealed either a simple insertion loop at the position of the EcoRI site of the parental plasmid DNA. We provide evidence to suggest that these deletions are the result of a site-specific nicking activity of the EcoRI preparations used in the formation of recombinant plasmids.

DNA Restriction Enzymes↗
PubMed1977

Genes for gamma-globin in human adult erythroid DNA.

Purified gamma-globin specific complementary DNA has been used to demonstrate the presence of the gene for gamma-globin in DNA from human adult red blood cells. This finding sheds doubt on any theory involving looping-out excision of genes to explain the switch over from synthesis of gamma-globin to beta-globin at birth.

Adult↗
PubMed1976

Delta-beta-thalassemia is due to a gene deletion.

DNA has been prepared from peripheral blood or cultured skin fibroblasts obtained from three Sicilian and one Greed deltabeta-thalassemia homozygotes. Globin-gene analysis was carried out using a cDNAbeta probe, and the results indicate that deltabeta-thalassemia has arisen from a deletion of the beta-globin genes. A similar result was obtained using DNA prepared from cultured skin fibroblasts from an individual homozygous for the Negro form of hereditary persistence of fetal hemoglobin (HPFH). In both cases, the deletion has spared the Ggamma and Agamma loci directing the gamma chains of hemoglobin F, but it has not been possible to demonstrate any difference between the size of the deletion involved in the production of delta-beta-thalassemia and that which gave rise to HPFH. These experiments provide further direct evidence that deletions of critical areas of the gamma-delta-beta gene cluster result in persistent gamma chain synthesis in adult life.

Chromosome Aberrations↗
PubMed1975

The activation of RNA polymerase by alkylammonium ions.

Tetramethylammonium and tetraethylammonium ions both lower the melting temperature of DNA. We have shown that these ions increase the activity of E. Coli RNA polymerase. When the base composition of the DNA template was varied, there was a correlation between the decrease in melting temperature and increase in RNA polymerase activity. No stimulation was observed when heat denatured DNA was used as template. It was shown that initiation of RNA synthesis was stimulated but to a smaller degree than was total RNA synthesis. The lag observed at low temperatures before RNA synthesis commences was found to be shorter in the presence of these alkyl ammonium ions. All these results are consistent with an unwinding of the double helix being an important step in transcription.

Animals↗
PubMedDate not supplied

Discipline-specific inquiry: the hermeneutics of theory-guided nursing research.

Gadamer's insights into the hermeneutical process contribute a great deal to the understanding that may accompany scientific inquiry, if preexisting prejudices are respected for their contribution to discipline-specific knowledge. Heidegger said that language "speaks for us in what has been spoken." This means that the way nurses use language signifies who they are as a group of health professionals. Language is the vehicle that discloses nurses' values and beliefs. Parse's theory is one framework among many that provides nurse researchers with a coherent language of the human-health interrelationship for guiding inquiry. The prejudices of the theory are clearly specified and they circumscribe unique arenas for knowledge development. The language of the theory describes human becoming from a nursing perspective. It is complex, process oriented, and abstract, just like the human-health phenomena it represents. It is precisely the unique understanding and knowledge of nursing theories that may direct further discipline-specific inquiry and structure activities for an autonomous nursing practice. Ultimately, knowledge generated from discipline-specific inquiry will expand theories for guiding creative and meaningful practice with persons, families, and groups that seek professional engagements.

Humans↗