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Biomedical subjects

G J Marell

Publications and source records attributed to G J Marell.

5 recordsLinked to original sources

Laboratory work flow analysis and introduction of a multi-functional analyser.

Laboratory work flow analysis was performed in order to define guidelines for improved laboratory organisation and efficiency. All activities were monitored from the moment a laboratory test was requested until the result was reported and received. Detailed information was collected on numbers of samples and tests, work-stations and sample splitting, number of staff and laboratory costs and management. From the data thus obtained requirements for optimal reorganisation could be developed. Reduction of work-stations appeared to be of primary importance. This could be achieved by replacement of seven different work stations by instruments for multi-functional analysis (Cobas Integra) in the department of routine clinical chemistry. Effects of reorganisation were evaluated by repeated work flow analysis. The multifunctionality of the analysers (photometry, turbidimetry, ion selective electrodes and fluorescence polarisation) provides opportunities for efficient work structuring, avoiding the need for sample splitting, distribution of sub-samples and performing analyses at different work stations. Manual and clerical errors could thus be reduced. Laboratory service to clinicians was improved by reduction of turnaround times to such an extent that all test results are reported within 60 minutes (stat service) even during peak hours. Laboratory costs were reduced by decreasing the number of laboratory staff and work-stations. Both clinicians and patients expressed great satisfaction with the effects of this reorganisation, for which work flow analysis appeared to be an indispensable instrument.

Blood Chemical Analysis↗

Quantitative determination of non-haem iron and ferritin iron in bone marrow using flameless atomic absorption spectrophotometry. A comparative study on the cytological and chemical determination of the bone marrow iron content.

A method for non-haem iron analysis in bone marrow aspirates using graphite furnace atomic absorption spectrophotometry has been developed. Bone marrow aspirates were obtained from patients with various disorders. A good correlation is observed between chemical and cytological assessment of total non-haem iron in bone marrow. An intra-assay coefficient of variation of 9.0% was observed. The ferritin-iron concentration was also determined and a CVduplo of 11% was found. The ferritin iron concentration increased with an increasing total iron content until saturation of ferritin appeared to be reached at about 3 g ferritin per kg protein. It was concluded that the quantitative determination of bone marrow iron can be of value in the diagnosis and investigation of both hypo- and hyper-ferraemic disorders.

Bone Marrow↗

Rapid and sensitive immunochemical determination of immunoglobulins by centrifugal analysis.

The measurement of immunoglobulin concentrations in serum by a turbidimetric method using a centrifugal analyser (Cobas Bio) is described. In the procedure described it is possible to measure the absorbance of each sample in the buffer reagent in a preliminary run without antiserum (sample blank for correction of colour, turbidity, etc.) In the main run (after adding antiserum) a final measurement is made after 600 seconds reaction time and the difference between the final absorbance and the sample blank is corrected for the absorbance of the antiserum determined in the antiserum blank cuvet. The initial phase of the reaction can thus be taken into account so that high titre antisera can be used. Therefore the method covers a wide range of values with good precision.

Antigen-Antibody Complex↗