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Biomedical subjects

G J Calton

Publications and source records attributed to G J Calton.

At least 73 records · Page 4Linked to original sources
PubMed1981

Immobilization of Escherichia coli Cells Containing Aspartase Activity with Polyurethane and Its Application for l-Aspartic Acid Production.

Whole cells of Escherichia coli containing aspartase activity were immobilized by mixing a cell suspension with a liquid isocyanate-capped polyurethane prepolymer (Hypol). The immobilized cell preparation was used to convert ammonium fumarate to l-aspartic acid. Properties of the immobilized E. coli cells containing aspartase were investigated with a batch reactor. A 1.67-fold increase in the l-aspartic acid production rate was observed at 37 degrees C as compared to 25 degrees C operating temperature. The pH optimum was broad, ranging from 8.5 to 9.2. Increasing the concentration of ammonium fumarate to 1.5 M from 1.0 M negatively affected the reaction rate. l-Aspartic acid was produced at an average rate of 2.18 x 10 mol/min per g (wet weight) of immobilized E. coli cells with a 37 degrees C substrate solution consisting of 1.0 M ammonium fumarate with 1 mM Mg (pH 9.0).

Journal Article↗
PubMed1981

Use of IgE antibody determinations in cutaneous Coelenterate envenomations.

Elevated levels of serum anti-sea nettle venom IgE persisted for several years in two patients who had exaggerated reactions to jellyfish (sea nettle, Chrysaora quinquecirrha) stings. These antibodies also cross reacted with Portuguese man-of war venom. The determination of increased antigen specific IgE concentrations may be of value in identifying patients "at risk" or in diagnosing stings by "unseen" animals. Because of the increased frequency of IgE radioallergosorbent testing (RAST) in dermatologic practice, a brief review of the use of this test is included.

Adult↗
PubMed1980

Use of the radioallergosorbent test for the study of coelenterate toxin-specific immunoglobulin E.

The radioallergosorbent test (RAST) was adapted for use to screen the immune response to sea nettle (Chrysaora quinquecirrha) and Protuguese man-o'-war (Physalia physalis) toxins. The results of a preliminary screening of serum from patients varying in their reaction to envenomations indicate the potential of these toxin proteins to induce an allergic state in humans and illustrate the use of the RAST as a screening device to detect persons sensitive to coelenterate stings.

Adult↗