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Biomedical subjects

G I Kozinets

Publications and source records attributed to G I Kozinets.

At least 19 recordsLinked to original sources
PubMed2002

[Evaluation of NMR relaxation method as a diagnostic tool for donor blood analysis and patients with hematologic diseases and burns].

Diagnostic value of the NMR-relaxation method in the blood plasma was estimated in the patients with different pathologies. The time of hydrogen nuclei longitudinal relaxation (T1) in the health donors of the blood, in the patients with oncopathology (hemoblastoses) and in the cases with anemia and burning disease were investigated. The time of the longitudinal relaxation (T1) was measured by automated NMR-relaxometer "Palma" (Russia). The working frequency was equal to 35 MHz, the temperature was 45 +/- 0.1 degrees C. For the single measurement 0.2 ml of blood obtained from heparinized venous blood 1.5 hours after its taking was used. The time of the longitudinal relaxation (T1) was shown to be 1.78 +/- 0.02 in the health donors, 1.70 +/- 0.06 s in cases with anemia, 1.97 +/- 0.48 c in patients with leucosis, 2.40 +/- 0.12 s in patients with burns. The sensitivity and the specificity of diagnostics of leucosis based upon the results of the only single T1 measurement in blood plasma were concluded to be 75%. It proves the significant T1 change both in patients with anemia and burning disease of the II-III degree. However it is evidently insufficient for selective use of NMR-relaxation blood plasma (serum) in the diagnostics of anemia and leucosis. The data obtained prove also the possibility of use of NMR-relaxation blood plasma (serum) for control of the hemostasis state during treatment or remission.

Anemia↗
PubMed2002

[Ratio of erythrocyte number to erythrocyte size in peripheral blood].

The count and size of erythrocytes are normally stable. In order to evaluate their ratio in health, some hematological diseases and during exposure to some ecological factors, peripheral blood erythrocytes were studied in 747 subjects (115 healthy controls aged 18-80 years, 56 subjects chronically exposed to ionizing radiation sources, 105 subjects working with aggressive liquids, 117 patients with anemias, and 81 with Hodgkin's disease). No relationship between erythrocyte count and MCV was detected in normal subjects and patients with anemia and Hodgkin's disease. It seems that this relationship is rather specific for hemopoiesis shifts associated with cell cycle delay, as it was detected in patients with B12 and folic deficiency anemias, in subjects chronically exposed to ionizing radiation sources, and in those handling nitrogen oxide in combination with heptyl.

Adolescent↗
PubMed2002

[Microvzor-2: a system for automated dry blood smear analysis].

Upgraded system Microvzor-2 for investigation of blood morphology in space flight is composed of soft- and hardware for dry smear image analysis. Ground-based testing showed that it could be utilized to investigate a broad spectrum of blood parameters in space flight, including erythrocytes, hemoglobin content in erythrocytes, volumetric erythrocyte distribution, diagnostics of anisocytosis, and poikilocytosis, leukocytes and leukocyte count. Analyzed are smears prepared from a fixed volume of finger blood. The process consists of smear scanning, input of the vision field images in the spacecraft computer and ensuing automated identification and counting. Information is stored in a dedicated database. Results of analysis are displayed as forms and cell galleries.

Adaptation, Physiological↗
PubMed2001

[Magnetic resonance spectroscopy].

Life-time diagnosis of the initial pathological shifts in the organism remains an and still unsolved important problem of modern medicine. Magnetic resonance spectroscopy based on the nuclear magnetic resonance phenomenon is one of the methods which can help to solve this problem. Modern reports about the use of magnetic resonance spectroscopy in the diagnosis of various diseases are reviewed.

Diagnostic Techniques and Procedures↗
PubMed2001

[Dynamics of erythrocyte electrophoretic mobility in patients with various forms of erysipelas].

AIM: To study electrophoretic mobility (EM) of erythrocytes in the course of various forms of erysipelas. MATERIAL AND METHODS: 78 patients with various erysipelas clinical forms were examined in the disease acute period and early in convalescence. RESULTS: EM of erythrocytes was significantly higher in hemorrhagic forms of erysipelas vs an erythematous form. CONCLUSION: Manifest changes in functional condition of erythrocytic membranes in patients with erythematous-hemorrhagic and bullous-hemorrhagic forms of erysipelas play an important part in the development of local hemorrhagic syndrome.

Adolescent↗
PubMed2001

[Various aspects of bone marrow cell proliferation in acute leukemia].

AIM: To study proliferation of bone marrow cells in acute leukemia using flow DNA-cytometry. MATERIAL AND METHODS: Parameters of the cell cycle (S, G2 + M, G1/0) and myelogram were studied in 55 patients with acute myeloid leukemia (AML) and 46 patients with acute lymphoblastic leukemia (ALL) in remission (135 cases) and acute period (66 cases). Only cases with cell clones containing normal (diploid) number of DNA were included. RESULTS: In acute period, distribution of AML cases by the percentage of cells in S-phase of the cellular cycle was normal, in ALL--log-normal. In remission it was lognormal in both leukemias. Variability of cell cycle parameters in leukemia in some patients was much less than a mean sample one. The reestablishment of proliferation after chemotherapy (7 + 3) in AML was followed up. CONCLUSION: If proliferative activity of blast (tumor) cells in AML is subnormal (10%), proliferation of myelokaryocytes can be used as an additional test for assessment of remission completeness.

Acute Disease↗
PubMed2000

[Comparative assessment of myelocariocytes cell cycle in patient with hemodepressions].

The proliferative activity of bone marrow cells was studied by flow cytofluorometry in 8 patients with aplastic anemia (AA), 6 with myelodyspastic syndrome (MDS), and 26 normal subjects aged 21-40 years. The myelokaryocyte proliferation was inhibited at the G0-1 stages of cell cycle, the number of cells in the S phase was decreased, and the coefficient of variations cV of DNA cytofluorometry values were increased in AA patients in comparison with the norm. By contrast, in MDS patients the number of cells in the G0-1 phase was decreased and that of cells in the S phase increased in comparison with the norm and with AA patients; cV was increased. These differences in the cell cycle characteristics are suggested to be used as a differential diagnostic criterion in AA and MDS.

Adult↗
PubMed2000

[The erythron system in body exposure to nitrogen oxides].

AIM: Assessment of erythron parameters in subjects exposed occupationally to nitrogen oxides polluted air versus those who work in healthy environment. MATERIALS AND METHODS: Levels of red cells, reticulocytes, packed cell volume, resistance of red cell membranes to acid buffer were measured in 95 subjects working in polluted environment and 89 healthy controls. RESULTS: Long-term (10 years and longer) contact with nitrogases entails a tendency to lowering of red cell number in the exposed subjects, significant rise of packed cell volume, increased mean cell volume of red cells, anisocytosis and reticulocytosis, high acid resistance of red cells and their membrane permeability. The causes of these changes are discussed in terms of "uneffective erythropoiesis" conception. CONCLUSION: Thus, erythron parameters can serve for indication of toxic effects of some chemicals on human body.

Adult↗
PubMed2000

[Comparative study of platelets from healthy human donors using light microscopy and flow cytometr "Cobas micros"].

Peripheral blood platelets of 25 normal subjects were examined by optic microscopy and flow cytometry. Blood was collected routinely into tubes for Cobas Micros flow scintillator with EDTA anticoagulant. Smears were made as a monolayer and stained by May-Grünwald's method in our modification. Morphologically, 5 classes of platelets were distinguished under optic microscope: young, mature, old, macroplatelets, and irritated forms. The percentage of each class was estimated (i.e., normal platelet formula was determined). Comparison of platelet parameters obtained on a flow cytometer and optic microscopy data for subjects with high percentage of large platelets showed increased mean volume of platelets (MPV) and width of platelet volume (PDW). The curve reflecting platelet distribution by volume in these donors was characterized by strong inclination to the right and a flat plateau.

Adult↗