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Biomedical subjects

G Husby

Publications and source records attributed to G Husby.

At least 271 records · Page 15Linked to original sources
PubMed1976

Tissue T and B cell infiltration of primary and metastatic cancer.

Immunofluorescent techniques were utilized to identify the types of infiltrating lymphocytes adjacent to human malignant tumors arising from a wide range of anatomic sites. 24 of 29 primary tumors and 5 of 8 metastatic lesions showed varying degrees of lymphocytic infiltration. T cells predominated in the infiltrates in primary tumors (mean 80%, range 50-100%) and this pattern was evident regardless of anatomic site or the presence or absence of metastatic spread. By contrast, B cells predominated at the margins of three of five tumor metastases. Mononuclear cells bearing the Fc receptor were not a prominent component of the infiltrates associated with either primary tumors or metastases, but tumor cell binding of fluoresceinated IgG aggregates was observed in 12 of 29 primary tumors. A significant reduction in peripheral blood T cell numbers occurred in a third of the patients studied. This decrease was not clearly related either to the extent of local tumor T cell infiltration or to the presence of disseminated disease. These preliminary findings provide a descriptive analysis of the local and systemic distributions of immunocompetent cells in cancer.

Adult↗
PubMed1976

Liver disease--a prominent cause of serum IgE elevation.

Serum IgE concentrations were elevated in thirty-seven out of sixty-seven patients (55%) with acute or chronic liver disease of widely differing aetiology. The mean IgE concentrations in these patients showed an eight-fold increase above that observed in control subjects. Increased IgE levels in patients with liver disease occurred in the absence of eosinophilia, clinical evidence of atopy or other known causes of IgE elevation. No IgE-containing plasma cells were detected in the liver biopsies from thirty-two of the sixty-seven patients tested. Peripheral blood T cells were significantly decreased from normal in the patients with liver disease, but no correlation emerged between serum IgE levels and absolute peripheral blood T-cell numbers. These findings emphasize the importance of liver disease as a significant cause of serum IgE elevation.

Adult↗
PubMed1976

Amyloid fibril protein AA in Papua New Guinean amyloidosis.

In this study of protein composition of amyloid fibrils isolated from eight patients representative of the spectrum of amyloidosis found in Papua New Guinea has been investigated. All fibril preparations, including three from patients with amyloidosis secondary to lepromatous leprosy and one from an unusual juvenile case of primary amyloidosis, contained the non-immunogobulin amyloid protein, protein AA. However, only 44% of thirty-six amyloid patients had detectable levels of the protein AA-related serum component, protein SAA. Alkali-degraded material from each of the fibril preparations failed to react in double immunodiffusion test with antiserum to the amyloid-related light chain VgammaV, but evidence was found for this immunoglobulin light chain-specificity in the serum of one patient.

Adult↗
PubMed1975

[Ibuprofen (Brufen)].

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Anti-Inflammatory Agents↗
PubMed1975

Isolation and characterization of amyloid-related serum protein SAA as a low molecular weight protein.

With direct immunoprecipitation or gel filtration under dissociating conditions, amyloid-related serum protein SAA has been isolated as a low molecular weight protein from the serum of two patients with rheumatoid arthritis but without known amyloidosis. The isolated protein SAA showed antigenic identity and an amino acid composition that was similar, but not identical, with isolated fibril protein AA. Molecular weight estimations suggest that protein SAA is approximately 50% larger than protein AA and has a molecular weight of 14,000-15,000 daltons. Preliminary results indicate that protein SAA from a patient with amyloidosis has a similar small molecular weight subunit.

Adolescent↗
PubMed1975

An experimental model in mink for studying the relation between amyloid fibril protein AA and the related serum protein SAA.

Experimental amyloidosis was induced in mink by repeated injections with endotoxin. Amyloid fibrils extracted from liver and spleen were fractionated by gel filtration after treatment with guanidine-hydrochloride and a reducing agent, dithiothreitol. An elution profile very similar to that of human amyloid fibrils, having protein AA as a major component, was obtained. The mink amyloid protein eluted at a position similar to that of human protein AA was by amino acid composition and partial sequence studies shown to be very similar to the latter protein and was called mink protein AA. In addition, a protein AA-related component (protein SAA) was found in increased amounts in serum of amyloidotic mink, providing further evidence of the homology with human amyloids. Experimental amyloidosis in mink represents a suitable model for studying amyloid proteins and related serum components.

Amino Acid Sequence↗
PubMed1975

Amyloid-related serum component (protein ASC) IN LEPROSY PATIENTS.

The presence of amyloid-related serum component, protein ASC, in serum samples from 63 leprosy patients was investigated. Protein ASC was detected in 38% of the patients. A correlation to the disease spectrum of leprosy was apparent: polar lepromatous cases, 64% positive; borderline lepromatous, 50%; borderline tuberculoid, 36%; subpolar tuberculoid, 17%; and polar tuberculoid, negative. Antibody activity against the a antigen of Mycobacterium leprae was also determined, showing a similar correlation to the disease spectrum. Serum samples from 23 apparently healthy Ethiopians serving as controls showed a protein ASC incidence of 22%. This figure is significantly higher than the frequency found by others among healthy Norwegian blood donors. Immunoglobulin M levels among patients were elevated in the borderline lepromatous and poplar lepromatous groups. The three tuberculoid groups did not differ in this respect from the control group but were all elevated as compared to a normal Caucasian serum pool. Although raised immunoglobulin M levels seemed to parallel increased frequencies of protein ASC in the patient groups as well as in controls, this correlation might be only secondary to a primary derangement in T-cell function.

Adult↗
PubMed1975

Peripheral blood and intestinal lymphocyte sub-populations in Crohn's disease.

Immunofluorescent technques were utilized to study the nature and distribution of cells forming the inflammatory infiltrate in Crohn's disease. Tissue from resected ileum (one patient) or colon (one patient) when compared with corresponding control tissues contained increased numbers of B and T lymphocytes. B-cells of IgG class predominated in the mucosal lamina propria, whereas T-cells were prominent in the deeper layers of inflamed bowel. Inflammatory cells bearing Fc receptors were also present in these tissues. Decreased peripheral blood T-cell numbers returned to normal after resection of the involved bowel in the two patients studied. Impaired cellular immunity in Crohn's disease may thus result from T-cell sequestration in or loss from involved segments of intestine.

Adolescent↗
PubMed1975

Localization of T and B cells and alpha fetoprotein in hepatic biopsies from patients with liver disease.

Peripheral blood and hepatic tissue T- and B-lymphocyte distributions, serum alpha fetoprotein (AFP) concentrations, and hepatic AFP were studied in 46 patients undergoing diagnostic percutaneous liver biopsy. The patients included 26 with alcoholic liver disease, 13 with nonalcoholic hepatitis or cirrhosis, and 7 with either normal histology or minor nonspecific changes. Serum AFP was determined by radioimmunoassay and hepatic tissue AFP by indirect immunofluorescence. Peripheral blood T lymphocytes were identified by the sheep red-cell rosette technique; and B lymphocytes by fluoresceinated anti-immunoglobulin antisera and IgG aggregates. Tissue identification of T lymphocytes was accomplished using an extensively absorbed rabbit antihuman thymocyte antiserum and indirect immunofluorescence; tissue B lymphocytes were identified using pepsin F (ab')2 fragments of rabbit IgG antibodies to human immunoglobulins. T lymphocytes predominanted in hepatic lymphoid infiltrates from patients with alcoholic liver disease (91+/-4%), whereas in patients with chronic active or chronic persistant hepatitis, viral hepatitis, or cryoptogenic cirrhosis proportions of T and B lymphocytic infiltrates were similar (50+/-15%). Hepatic tissue AFP was detected in 9 of 18 patients with alcoholic hepatitis; serum AFP concentration was increased in only 1 of these 9 patients. Tissue AFP was not observed in the remaining biopsy material nor were serum AFP concentrations increased. Peripheral blood T-cell numbers were significantly decreased in patients with alcoholic liver disease (P less than 0.01) and in nonalcoholic hepatitis or cirrhosis (P less than 0.025). A close relationship between peripheral blood T-lymphocytopenia and hepatic T-cell infiltrates was observed in patients with alcoholic liver disease; this relationship was less apparent in patients with nonalcoholic hepatitis or cirrhosis.

B-Lymphocytes↗
PubMed1975

Occurrence of amyloid in the teeth-supporting tissues of patients with rheumatoid diseases. An immunohistochemical study.

An investigation was carried out to determine whether amyloid could be detected in the dental periapical lesions or gingival buccal fold, or in both, of patients with rheumatoid disease but in whom amyloidosis had not been diagnosed. Tissue from the dental periapical lesions of 36 rheumatoid and 23 control patients, as well as from the gingival buccal fold of 11 of the rheumatoid and 11 of the control patients, were examined by the direct immunofluorescence technique and by polarization microscopy of sections stained with Congo red. Amyloid was observed almost 5 times more often, or in 19% of the dental periapical lesions of rheumatoid patients: it was detected by immunofluorescence in 7 rheumatoid patients and 1 control patient, and by polarization microscopy in 6 of these 7 rheumatoid patients as well as in the 1 control. Rectal biopsies performed on these 8 patients were positive in only 1 of the rheumatoid patients. Amyloid was not detected in any of the gingival biopsies. Serum samples from the rheumatoid and the control patients were tested for the presence of the nonimmunoglobulin amyloid fibril-related serum component, protein ASC. Nineteen of the 36 rheumatoid patients, including the 7 whose dental periapical lesions contained amyloid, had protein ASC in their serum.

Adult↗
PubMed1975

Comparative double-blind trial of intra-articular injections of two long-acting forms of betamethasone.

Two injectable betamethasone preparations, betamethasone dipropionate/betamethasone phosphate and betamethasone acetate/betamethasone phosphate were compared in a controlled trial on 22 "matched patient pairs" with rheumatic diseases. The two preparations were injected intra-articularly in knee joints affected by active synovitis. The time until onset of action and the duration of clinically adequate anti-inflammatory effect were recorded. All injections produced clinically significant effects. The interval before recurrence of symptoms varied from 2 days to more than 3 months. The dipropionate form showed statistically significantly longer duration of action. No difference was noted in the time until onset of action. Local side effects were not observed. The double-blind method of clinical evaluation proved useful in comparing duration of action between long-acting corticosteroids.

Adult↗
PubMed1975

Smooth muscle antibody in heroin addicts.

The occurrence of autoantibodies and the concentrations of serum immunoglobulins were studied in 102 heroin addicts, 20 former addicts who had abstained from heroin for 1 year or more, and 40 normal control subjects. Antibodies to smooth muscle (46%) and lymphocytotoxic antibodies (30%) were detected in the active heroin users, and there was a significantly positive correlation between these autoantibodies. Absorption experiments strongly suggested antigenic cross-reactivity between smooth muscle and lymphocyte membrane antigens. The presence of smooth muscle antibody in addicts was not clearly correlated with signs of active liver disease. The occurrence of smooth muscle antibody (10%) and lymphocytotoxic antibodies (15%) was significantly lower in the former heroin addicts than among active drug users. A significant elevation of IgM was found in the active heroin addicts. Gamma-M globulin was lower among the former heroin addicts but still elevated when compared with normal controls.

Antibodies↗
PubMed1975

Amyloidosis in rheumatoid arthritis.

The clinical and pathological features of amyloidosis are reviewed with special references to amyloidosis associated with rheumatoid arthritis. Data concerning the nature of the amyloid substance are presented. The amyloid fibril which is the unique and principal component of all amyloid substances, can be constituted by different protein subunits. The amyloid fibril protein AA is a major subunit of the amyloid fibrils particularly seen in secondary amyloidosis including that associated with rheumatoid arthritis. The immunological and chemical properties of protein AA and a structurally related serum protein (SAA) are discussed. Increased knowledge of the nature of amyloid and its precursor proteins may provide better methods for the diagnosis and treatment of amyloidosis.

Amino Acid Sequence↗
PubMed1975

Immunofluorescence studies in herpes gestationis.

In 4 cases of H.G., direct junctional immunofluorescence findings showed an intense band of C3 present in the involved skin of the patients even after the active lesions had disappeard (i.e. 2 months later). Weak specific fluorescence for IgG and in one case also for IgA and IgM was seen in the involved skin. A less intense junctional band of C3 was also present in the uninvolved skin. Antibodies against basal membrane have not been detected in the sera of the patients.

Adult↗
PubMed1975

Deposition of fibrinogen (FR-antigen) in skin diseases. I. Psoriasis vulgaris and Psoriasis arthropathica (with special reference to heparin-precipitable fraction).

Twenty-five in-patients with psoriasis arthropathica (ps.a.) and 18 in-patients with psoriasis vulgaris (ps.v.) were examined for cryofibrinogen as heparin-precipitable fraction (HPF), for total fibrinogen in plasma and by immuno-fluorescence (IF) technique for FR-antigen in tissue sections of affected and unaffected skin. A control group of 14 in-patients with various non-psoriatic dermatoses were examined with if for FR-antigen in unaffected skin. Pathological quantities of HPF were found in 6% in ps.v. and in 48% in ps.a. Total fibrinogen in plasma was normal in ps.v. (365 mg%), and moderately elevated in ps.a. (471 mg%). In psoriatics, FR-antigen was found in unaffected skin ps.v. in 50%, in ps.a. in 72%. In affected skin, the figures were 67% and 84% respectively. No direct relationship could be found between the incidence and the degree of HPF, and depositions of FR-antigen in the tissue sections in the skin at the time of the examination. In the control group, no FR-antigen could be found in unaffected skin, except in one case of acne cystica, with traces of FR-antigen in a few areas of the tissue section.

Adult↗
PubMed1975

Gastrointestinal investigations in dermatitis herpetiformis.

Thirty-seven patients with dermatitis herpetiformis (DH) have been investigated for gastric and small intestinal abnormalities. Evidence of an enteropathy was found in 86% of the patients who had IgA deposits in uninvolved skin. Villous atrophy of the small intestine was found in 29 patients. About one-half of the patients had reduced absorption of xylose and vitamin A. The Schilling test value was lowered in one-third. Serum B12 was too low in 5/28 patients whereas folic acid in serum and whole blood was too low in 14/29 and 5/19, respectively. Atrophic gastritis occurred in 14/28 patients and only about one-third of the whole patient material had normal gastric mucosal structure and secretion.

Adolescent↗