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Biomedical subjects

G Holm

Publications and source records attributed to G Holm.

320 records · Page 18Linked to original sources
PubMedDate not supplied

Surface immunoglobulin pattern of the leukaemic cell population in chronic lymphocytic leukaemia (CLL) in relation to disease activity.

The surface membrane immunoglobulin (smIg) isotype pattern of the leukemic lymphocytes was studied in 66 unselected patients with CLL. Five distinct patient groups were identified according to the dominant heavy chain isotype(s); I: smmu + (n = 22), II: smmu +/smdelta + (n = 25), III: smdelta + (n = 4), IV: smgamma + (n = 5), V: no detectable heavy chains (n = 10). The majority of group I patients had a progressive disease at test while all patients in group II were in a non-progressive state. Moreover, the smIg pattern changed with the clinical activity of the disease: when the disease progressed, the relative number of smmu + cells increased and when patients entered an indolent stage after treatment the smmu + cell population decreased. In patients with stationary disease the smIg pattern remained essentially unchanged or the relative number of smdelta + cells increased. These observations might suggest that the smIg isotype pattern of the leukemic cell population reflects the biological behaviour and the clinical activity of the disease.

Adult↗
PubMedDate not supplied

Blood lymphocyte characteristics as predictors of prognosis in chronic lymphocytic leukemia of B-cell type.

The prognostic information of blood lymphocyte characteristics and clinical findings was assessed in 62 patients with chronic lymphocytic leukemia of B cell type. Bivariate and multivariate survival analyses were performed using age, Rai stage, surface membrane immunoglobulin (smIg) isotype pattern of the leukemic clone, total lymphocyte counts, numbers of proliferating lymphocytes and T cell subpopulations. Rai stages III and IV, high numbers of blood lymphocytes in S-phase (S+) and sm mu isotype were found to be partly independent factors predicting short therapy-free survival. Patients with a sm mu+ leukemic cell clone had a shorter therapy-free and total survival compared to those with sm mu+/delta+ and sm delta+ leukemic cells. Moreover, patients with high numbers of blood S+ lymphocytes had a shorter therapy-free and total survival compared to those with few S+ cells. These prognostic variables were valid also in patients with a low tumour burden (Rai stages 0, I and II) and may thus be of clinical importance as a guideline for therapeutic intervention.

Actuarial Analysis↗
PubMedDate not supplied

Immunodeficiency and prognosis in patients with non-Hodgkin's lymphomas.

Monocyte depleted blood lymphocyte subpopulations, their functions and relation to prognosis were studied in 68 untreated adult patients with non-Hodgkin's lymphomas classified according to the Kiel nomenclature. The median observation time was 48 months (range 41-60). The mean total blood lymphocyte and T (SRBC-rosetting) cell counts were significantly decreased as compared with age-matched controls (n = 57). Twenty-five per cent of the patients had a monoclonal blood B lymphocyte population. The spontaneous lymphocyte DNA synthesis, measured as incorporation of 14C-thymidine, was increased and the response to mitogen and antigen stimulation was decreased. Blood lymphocyte counts and functions before treatment were not related to the rates of remission or survival.

Adult↗
PubMedDate not supplied

[Pregnancy diagnosis in thoroughbred mares using radioimmunoassay (RIA), enzyme immunoassay (kit) and ultrasound echography (scanning)].

On the basis of progesterone determination on plasma or blood after RIA and the kit method respectively and consecutive scanning performed on a total number of 31 mares the following features were demonstrated: The overall material shows that in 20 mares (64.5%) embryonic vesicles were demonstrated. Of these mares 16 have conceived after service in the 1st estrous cycle and 3 mares in the 2nd estrous cycle. 18 mares were scanned in the time interval 13th-26th day after the latest service, while 2 mares were scanned on day 46 and 41 respectively. A total of 14 scanning positive mares were examined for progesterone by the RIA as well as by the kit method. For these mares (100%) agreement was found between the progesterone analyses as well as with the scanning results. For 19 mares (100%) there was agreement between kit and scanning results. 15 RIA progesterone determinations are in agreement with the scanning results. Progesterone values after the RIA method performed on 15 scanning positive mares were in average 8.40 +/- 2.79 ng/ml plasma. A total of 9 out of 10 scanning negative mares have been examined by the RIA as well as by the kit method. In 6 of these mares with 7 estrous cycles agreement has been found between progesterone analyses and the scanning results (77.8%). In 2 mares (no. 2 and 4) discrepancy has been found in the 2nd estrous cycle between RIA, kit and scanning results. For 1 mare (no. 7) discrepancy has been found in the 1st and 3rd estrous cycle between RIA and scanning.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗