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Biomedical subjects

G Heinrich

Publications and source records attributed to G Heinrich.

At least 91 records · Page 5Linked to original sources
PubMed1979

Sequences at the somatic recombination sites of immunoglobulin light-chain genes.

The entire nucleotide sequence of a 1.7-kilobase embryonic DNA fragment containing five joining (J) DNA segments for mouse immunoglobulin kappa chain gene has been determined. Each J DNA segment can encode amino acid residues 96--108. Comparison of one of the five J DNA sequences with those of an embryonic variable (V) gene and a complete kappa chain gene permitted localisation of a precise recombination site. The 5'-flanking regions of J DNA segments could form an inverted stem structure with the 3'-non-coding region of embryonic V genes. This hypothetical structure and gel-blotting analysis of total embryo and myeloma DNA suggest that the somatic recombination may be accompanied by excision of an entire DNA segment between a V gene and a J DNA segment. Antibody diversity may in part be generated by modulation of the precise recombination sites.

Animals↗
PubMed1975

The action of ionizing radiation on DNA in the presence of quinacrine. I. UV absorption and fluorescence measurements.

It is shown by UV absorption and absolute fluorescence spectroscopy of solutions containing both DNA and quinacrine that the components experience mutual radio-protection due to scavenging of water radicals. From measurements at different ionic strengths it is inferred that quinacrine bound to DNA is more efficiently protected than the free compound. Furthermore, release of bound quinacrine from DNA is observed at higher doses.

Animals↗
PubMed1975

Continuous growth of mitogen-reactive B lymphocytes.

B-cell mitogens induce continous growth of murine lymphocytes in suspension cultures. Continuous growth is observed only at low cell densitiess, below 10(6) cells/ml, in regularly renewed medium containing beta-mercaptoethanol and a growth-supporting fetal calf serum. Continuous growth of B lymphocytes in culture appears to be favored by conditions under which maturation of Ig-secreting plaque-forming cells does not occur. On the other hand, maturation is observed whenever stimulated B cells cease to divide. This maturation leads to IgM secretion followed by secretion of IgG- or IgD-like molecules. Neither continuous growth of B cells nor the 'switch' to secretion of IgG- or IgD-like molecules requires the presence of T cells.

Animals↗