Biomedical subjects

G Haukenes

Publications and source records attributed to G Haukenes.

At least 91 records · Page 5Linked to original sources

PubMed1979

Measles virus specific precipitins in sera from patients with chronic active hepatitis.

Measles virus specific antibodies in sera from 29 patients with chronic active hepatitis have been examined using standard serological tests and immunoelectrophoretic techniques. Significantly raised CF antibody titres against measles virus antigen were demonstrated in 50% of the sera. The major immunoprecipitate obtained by immunoelectrophoresis was shown to contain aggregates of measles virus nucleocapsids. Antibodies against the haemagglutinin antigen of the virus envelope were also raised and were demonstrated by line immunoelectrophoresis.

Adolescent↗

PubMed1979

Sendai virus haemolysis: mechanism of the increased haemolysis obtained by pretreatment of the virus with antibody and complement.

Sendai virus is haemolytic against erythrocytes from various species. Pretreatment of the virus with antibody and complement (C) resulted in a sixfold increase in the haemolysis. The viral haemolysin (HL) could be inactivated by heating the virus at 37 degrees C for 5 h. HL-inactivated virus became haemolytic again when pre-treated with antibody and C. This acquired haemolytic capacity corresponded to the enhanced haemolysis shown by intact virus. It is concluded that the enhanced haemolytic activity is due to a C-mediated indirect haemolysis and is not dependent on the fusion process. The possibility of cell damage in vivo from virus-antibody-C complexes is discussed.

Antibodies↗

PubMed1977

Rubella reinfection.

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False Positive Reactions↗

PubMed1977

Demonstration of host antigens in the myxovirus memebrane: lysis of virus by antibody and complement.

Complement-mediated lesions developed in myxovirus membranes in the presence of antibody to host antigens. Intact virions reacted with antibody to the keratosulphate-like host antigen present in the spike layer. Spike-deprived influenza virus reacted in the same way when an antiserum to the Forssman glycolipid was used. The holes in the membrane varied considerably in size, but were otherwise similar to those found in erythrocyte membranes. This immune electron microscopy method proved useful for the localization of host material in viral membranes.

Antibodies, Viral↗

PubMed1977

Quantitative immunoelectrophoretic studies of the L and S antigens of vaccinia virus.

The L (10S) and S (7S) antigens of vaccinia virus have been characterized by their sensitivity to heat, to proteolytic enzymes and to periodate. The L antigen was heat labile and inactivated by proteolytic enzymes and periodate. Treatment with trypsin for 1 min indicated that the S antigen was built up of at least two protein subunits of about 3S and 5S. One of the subunits was highly sensitive to heat and to proteolytic enzymes. On further treatment with trypsin the other subunit was split in two components showing partial identity serologically.

Antigens, Viral↗

PubMed1977

Production of monospecific antisera against two vaccinia virus antigens.

Vaccinia virus antigen was prepared from infected rabbit lung fibroblast monolayers grown in Eagle's medium supplemented with serum obtained from the rabbit going to be used for immunization. Monospecific rabbit antisera against the L and S antigens were produced by immunization with immunoprecipitates cut out from the agarose after quantitative line immunoelectrophoresis using antigen produced in HeLa cells.

Antigens, Viral↗

PubMed1976

The vaccinia virus haemagglutinin: enzyme sensitivity and some physiocochemical properties.

Purified vaccina virus haemagglutinin (VHA) was found to be sensitive to trypsin both with regard to haemagglutinating capacity and antigenic property. Phospholipase C and lipase had no effect on the haemagglutination (HA). Both the HA and the antigenic property thus depend on the integrity of the protein part. The loss of HA after treatment with trypsin may be due to breakdown of an essential protein or to secondary rearrangement of the whole structure, including the lipid part. Cells infected with an HA negative mutant of vaccinia virus also lacked the antigenic part of VHA. The sedimentation constant of VHA from HeLa cells was about 50.

Epitopes↗

PubMed1976

Identification of antigens within the so-called vaccinia L-S complex by means of quantitative immunoelectrophoresis.

The so-called L-S antigen complex of vaccinia virus antigens was studied using gel diffusion and various immunoelectrophoresis methods. The low resolution power of the gel diffusion method and the inconsistency of the results obtained rendered this method unsuitable for identification of individual antigens and for comparative studies. The sensitivity and simplicity of counter current electrophoresis showed the superiority of this method for rapid diagnosis of pox-virus infections. Simple rocket or line electrophoresis could not be used to distinguish the antigens. The height of the individual rockets and the position of the lines were greatly influenced by the strain of virus and the antibody composition of the antiserum. Crossed and crossed-line electrophoresis gave distinct and reproducible patterns which may provide a basis for further studies of vaccinia precipitinogens. The presumed L-S antigen complex was composed of two distinct antigens. The L antigen was more negatively charged and showed an approximate S value of 10 as compared to 7S for the S antigen.

Antigens, Viral↗

PubMed1975

False positive rubella virus haemagglutination inhibition reactions: occurrence and disclosure.

A floatation centrifugation procedure is described by which antibodies and non-specific rubella virus haemagglutination inhibitors can be completely separated. Using this method it was shown that false positive reactions occur with the conventional pretreatments of sera for removing inhibitors, especially with the heparin-McCl2 procedure. Other methods for removal of inhibitors were examined in view of recent knowledge about the chemical nature of the inhibitors. Treatment with phospholipase C proved useful for disclosure of false positive reactions, while floatation centrifugation was the most sensitive method for demonstration of low antibody concentrations.

Antibodies, Viral↗

PubMed1974