[Malignant lumbar chordoma--problems in diagnosis and therapy].
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Aged↗
Biomedical subjects
G Haroske
Publications and source records attributed to G Haroske.
Some remarks on preinvasive cervical neoplasia--an image analysis study.
By means of automated microscope image analysis 5275 normal cells as well as dyscaryotic cells were investigated in cytologic preparations from 53 cervical smears (26 negative cases, 27 positive cases). In this way, visually not conspicuous differences between normal cell populations of positive and negative cases could be demonstrated with multivariate statistic analysis of discriminant features. The examination of this phenomenon in 2910 epithelial cells from tissue sections of portio conizations has shown that the subvisually changed epithelial cells originate from circumscript regions in the margin and surroundings of CIN-lesions. The biological role of these changes in the formal genesis of cervical neoplasias has been discussed.
Cell Differentiation↗
Metastasizing APUD cell tumours of the human gastrointestinal tract. Light microscopic and karyometric studies.
A total of 11 metastasizing gastrointestinal APUD cell tumours from biopsy and autopsy files were reclassified according to Soga and Tazawa as well as to WHO Histologic Classification of Tumours. The much higher proportion of APUD cell tumours in autopsies (11 cases from 1000 autopsies in comparison with 22 cases from 22 000 biopsies) demonstrate that the majority of them will not be discovered during the patient's life. EC cell carcinoids (type A) predominate in both non-metastasizing and metastasizing gastrointestinal APUD cell tumours. Metastases from EC cell carcinoids occurred only in regional lymph nodes and in the liver. The APUD cell tumours originating in the pancreas represent the most frequently metastasizing gastrointestinal carcinoids. Besides in the liver and in regional lymph nodes metastases from pancreatic APUD cell tumours were seen in the skin, the brain and the skeleton. One case with two (pancreatic, bronchial) competitive primary APUD cell tumours and a skin metastasis was studied by means of automated cell image analysis. Cell populations of these three tumour sites were characterized by morphometric and densitometric nuclear parameters. It could be demonstrated that the skin metastasis consisted of a cell population, which occurred as a subpopulation in the primary tumour of the bronchus. The results of karyometric investigations supported the hypothesis that single components of tumours can metastasis selectively.
Adolescent↗
Cytomorphometric characterization of NHL(non-Hodgkin's lymphomas) of low and high grade malignancy.
From semithin sections of 12 non-Hodgkin's lymphomas (3 lymphocytic, 3 centrocytic, 3 lymphoblastic, 3 centroblastic m.L.) morphometric parameters of nucleus and cytoplasm, including size, shape, roundness, number and size of nucleoli were measured. Statistical evaluation of the data showed that the lymphoma cells mainly differ in their size, less in their shape. Nucleolar parameters are of greater value than contour features in discriminating the four groups of NHL. With growing grade of malignancy an increasing nuclear and cellular polymorphism was observed. Moreover, measurement of the feature "maximal nuclear diameter" on paraffin sections from 42 further cases showed the possibility of morphometric discrimination of low and high malignant NHL in histopathological diagnostics.
Biopsy↗
Differentiation of human T and B peripheral blood lymphocytes by high-resolution cell image analysis.
Automated cell image analysis of light and electron microscopic pictures was used for differentiation of nonlabeled lymphocytes in blood smears and in smears of purified lymphocyte suspensions. The percentages of T and B lymphocytes were determined by a two-step rosette assay with sheep red blood cells (T cells) and an immunofluorescence assay with FITC-labeled antihuman globulin (B cells). Images from 1,400 Feulgen-stained and 12,000 Pappenheim-stained cells were analyzed. Various classification methods allowed two lymphocyte subpopulations to be discriminated at the light and electron microscopic levels on the basis of different visual and subvisual morphologic features. As found by immunologic methods, morphologically determined subpopulations corresponded to T and non-T cells, with no further differentiation of non-T cells into B or null cells possible. The results allow the conclusion that there are morphologic differences between human T and non-T cells, with the differences distinguishable from individual variations as well as from alterations induced by sample preparation.
B-Lymphocytes↗
Image processing in pathology. XI. Light microscopic discrimination of human peripheral blood lymphocyte subpopulations.
Non-labelled lymphocytes in peripheral blood smears and in smears of purified lymphocyte suspensions were differentiated in T- and non-T-cells (controlled by E-rosetting assay and surface immunoglobulin detection) by automatic image processing at the light microscopic level. By various classification methods it was found that these lymphocyte subpopulations differ in several visual and subvisual morphologic features. The morphologic differences between subpopulations were superimposed by individual deviations as well as by influences from the preparation. Single features were strongly affected by exogenic factors. Most of the different features are comparable with fine structural differences, as previously reported.
Adolescent↗
Image processing in pathology. X. Electron microscopic morphometric analysis of human lymphocyte subpopulations.
In the present paper, methods of automatic image processing were used for a fine structural differentiation of lymphocyte subpopulations in purified non-labelled lymphocyte suspensions from peripheral blood. It was found that lymphocyte subpopulations differ in several morphological features which have been generated from the whole cell as well as from the nucleus. In general, these differences are greater than those attributed to individual deviations as well as as to influences from the preparation. As found by immunological methods, the composition of morphologically determined subpopulations corresponds to T- and non- T-cells. A preliminary functional interpretation fo the morphological differences is discussed.
Adult↗
[A case of malacoplakia of the prostate (author's transl)].
A case of malacoplakia of the prostate gland in a 75-year-old man is reported. This special type of granulomatous prostatitis was diagnosed by histological examination of biopsyn material. There were typical Michaelis-Gutmann corpusculates within the inflammation area. The structure of these inclusion bodies was investigated by electron microscopy. Characteristic concentric ring-shaped structures were seen as well as corpusculates which are composed of irregular cristalloid material. Some Michaelis-Gutmann bodies were found to contain short cell membrane-like structures. By histochemical investigation, both the cells possessing inclusion bodies as well as the Michaelis-Gutmann corpusculates themselves gave positive acid phosphatase reactions. The case is described because of the rarity of the malacoplakia of the prostate gland and with respect to the differential diagnosis of malignant prostate gland disease.
Aged↗
[Comparative studies of carcinomas of the human oral mucosa and xenografts in the nude mouse with the aid of computer-supported microscope image analysis].
The complete preservation of the original characteristics of donor tumors after xenografting into nude mice is more and more doubtful. With the aid of traditional biological methods and computer-assisted microscope image analysis the original human tumor tissues, including primary and recurrent tumors and metastases, are compared with nude mouse-grown xenografts. Squamous cell carcinomas of the oral mucosa served as original tumor tissues. Tumor takes were observed in 7 out of 12 cases. 5 transplantable lines could be established. Primaries, recurrent tumors and lymph node metastases are suitable for transplantations into nude mice and yield a comparable success rate. With one exception complete conformity between the original human tumor tissues and nude mouse-grown tumors could be found regarding the degree of differentiation and keratinizing tendency. As to the growth pattern of xenografts characterized by continuous shortening of growth periods, certain variations in the biological properties due to selection processes cannot be excluded. The results of microscope image analysis based on 35 different parameters suggest important differences within the developmental tendency of human tumor tissues and nude mouse-grown xenografts.
Animals↗