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Biomedical subjects

G H Hinkle

Publications and source records attributed to G H Hinkle.

40 records · Page 3Linked to original sources
PubMed1978

Preferential localization of radiolabeled liposomes in liver.

Liposome formulations were studied to achieve an efficient entrapment procedure for the production of liposomes of 99mTc-pentetic acid. The entrapment efficiency was studied by separation of the product using column chromatography. The particle-size range of the prepared liposomes was evaluated using electron microscopy. Entrapment techniques and separation procedures led to a liposome preparation with particles in the colloidal size range (0.001-0.5 micron). Dramatic differences in the organ distribution of the liposome preparation in mice were produced when different particle-size ranges were injected. Liposomes eluted in the first fraction after the void volume led to a maximum uptake by the liver and spleen 10 min after intravenous injection. Liposomes from pooled fractions provided less than half of the activity in the liver, as did the narrow size range liposome preparation.

Animals↗
PubMedDate not supplied

Autoradiographic evaluation of radiolabeled monoclonal antibody B72.3 distribution in tumor and lymph nodes of adenocarcinoma patients.

Monoclonal antibody B72.3 recognizes a pancarcinoma antigen termed TAG-72 and is the MAb in "OncoScint CR/OV". Patients undergoing surgical resection of primary or metastatic colorectal or breast carcinoma or pseudomyxoma peritonei were injected i.v. with 125I-labeled MAb B72.3. Autoradiography identified the tissue distribution of the injected radiolabeled MAb B72.3. Immunohistochemical staining identified the corresponding spatial distribution of the target antigen, TAG-72. The labeling pattern seen using autoradiography closely matched the pattern that was observed using immunohistochemical techniques. This was especially notable in the mucin-containing compartments of the different tumors. The 125I-B72.3 was also found associated with the neoplastic cells, demonstrating a good penetration and specificity of the radiopharmaceutical through the tumor masses. The regional lymph nodes examined were the only tissues in which autoradiography and immunoperoxidase gave different results. In these specimens, the presence of TAG-72 antigen in the parafollicular area, as shown by immunoperoxidase, contrasted with the silver grain deposition, due to the 125I-B72.3, in germinal centers. These findings suggest differences in the clearance pathways of the TAG-72 antigen and B72.3 MAb.

Adenocarcinoma↗