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Biomedical subjects

G Grabner

Publications and source records attributed to G Grabner.

At least 109 records · Page 6Linked to original sources
PubMed1982

The influence of long-term treatment with timolol on human tear lysozyme albumin content.

The long-term effect of timolol maleate on lacrimal secretion was tested by investigating the concentration of lysozyme and albumin in tears before the start of treatment and after different follow-up periods. A total of 25 patients (45 eyes) were treated with 0.25% or 0.5% timolol as the only antiglaucomatous therapy over a 7.6 month mean observation period (2-13 months). No significant change was found either in the lysozyme or in the albumin concentration.

Adult↗
PubMed1982

Human tear lysozyme. A comparison of electro-immunodiffusion, radial immunodiffusion and a spectrophotometric assay.

In 116 persons (67 men and 49 woman) the tear fluid of both eyes was collected using paper discs and once more after topical anaesthesia. After weighing the discs and eluting the tear fluid, the lysozyme content was measured using two immunological methods [radial immunodiffusion (RID) and rocket immuno-electrophoresis (RIE)] and a spectrophotometric assay. Standard curves were established with purified human lysozyme and egg-white lysozyme. In addition, albumin was determined in all the samples by rocket immuno-electrophoresis. All lysozyme assays showed a high degree of correlation, the spectrophotometric technique giving significantly lower values (compared with RID and RIE) when human lysozyme was used for standardization. The lysozyme content of the tears did not correlate with the fluid uptake in the discs, but showed a significant decrease with age, which was abolished by topical anaesthesia. In contrast, albumin levels were dependent upon the weight and independent of the age of the patient. It is suggested that the determination of albumin as a "reference protein' might help in comparing the results of different tear sampling techniques used by various groups, which induce variable amounts of reflex tearing.

Adolescent↗
PubMed1982

Interstitial linear keratitis.

A 15-year-old boy and a 21-year-old man had linear, nonmigratory corneal inflammations in the anterior stroma. The recurrent lesions extended from corneoscleral limbus to corneoscleral limbus within the palpebral aperture. They healed spontaneously with some scarring and minimal vascularization but without loss of vision. The cause could not be determined and serologic tests for syphilis were negative in both cases.

Adolescent↗
PubMed1982

[The value of sonography in the follow-up of patients with transplanted kidneys].

42 cases with transplanted kidneys were examined by ultrasound. The results were compared critically with clinical and histological data. The ultrasound findings are identical with the other findings in 86%. Ultrasound therefore together with clinical laboratory and nuclear medical findings can in a very high percentage replace other high risk examinations like retrograde pyelography or angiography.

Acute Disease↗
PubMed1982

Corneal epithelial cell--derived thymocyte-activating factor (CETAF).

Supernatants of a primary rabbit corneal epithelial cell culture and an established corneal cell line (SIRC) were assayed for their ability to enhance mitogen-induced C3H/HeJ mouse thymocyte proliferation. Significant levels of thymocyte-enhancing activity were detected in supernatants from both primary cultures and SIRC. Maximal levels of activity were found after 48 to 72 hr of culture in serum-free medium with 1 X 10(5) cells/ml. When monolayers of SIRC were disrupted. supernatants of these cultures consistently contained levels of activity higher than those of undisrupted control cultures. When supernatants from SIRC cultures (both serum-free and containing greater than 10% fetal calf serum) were subjected to gel filtration on AcA 54 and Sephacryl S-200, corneal epithelial cell-derived thymocyte-activating factor was eluted as two major peaks, between mol. wt 95,000 and 55,000 and mol. wt. 30,000 and 15,000. These results indicate that corneal epithelial cells, similar to keratinocytes, produce an Interleukin 1-like activity lacking species specificity, which enhances the proliferative capacity of thymocytes. Therefore corneal epithelial cells may interact with the immune system through the production of this cytokine.

Animals↗
PubMed1981

[The effect of neural mediation on the immediate rise in intraocular pressure in glaucomatous eyes after cyclocryotherapy (author's transl)].

As a rule, there is a transient rise in intraocular pressure following cyclocryotherapy. The preoperative administration of retrobulbar anaesthesia to a group of patients significantly reduced intraocular pressure after cyclocryotherapy in this group as compared with a control group without anaesthesia. These results imply that the temporary rise in intraocular pressure after cyclocryotherapy is mediated by a neural pathway.

Ciliary Body↗
PubMed1981

Mediation of the ocular response to cyclocryocoagulation.

The possible roles of prostaglandins and a neural pathway in the disruption of the blood-aqueous barrier in the rabbit eye after cyclocryocoagulation were studied. Both the preoperative IV administration of the prostaglandin inhibitor acetylsalicylic acid and the application of retrobulbar and topical anesthesia reduced IOP and decreased breakdown of the blood-aqueous barrier, as measured by protein in the aqueous humour. These results imply that the acute response of the animal eye to cyclocryocoagulation is mediated partly by prostaglandins and partly by a neural component resistent an inhibitor of prostaglandin synthesis. When administered together, acetylsalicylic acid and ocular anesthesia yielded a further reduction in postoperative reactions and protein concentrations in the aqueous humour, but were unable to abolish the ocular response completely. The dual ocular mediation to cyclocryocoagulation is apparently due to the combined thermal and mechanical injury caused to ocular structures which synthesize prostaglandins and receive sensory innervation from the trigeminal nerve. The considerable breakdown of the blood-aqueous barrier in cyclocryocoagulation allows leakage of different molecular weight proteins, in equal ratio, into the aqueous humour.

Animals↗
PubMed1981

3H-thymidine autoradiography of guinea pig cornea and skin after exposure to solar simulating radiation.

In vitro autoradiography with tritiated thymidine was performed in guinea pig cornea and guinea pig skin after in vitro exposure to solar simulating radiation (UVB). UVA-irradiated and unirradiated specimens served as controls. Sparsely labelled nuclei, indicating the unscheduled thymine dimer repair DNA-synthesis (dark repair), were observed immediately after UVB exposure in the epidermis and upper dermis of the skin and in all cellular compartments of the cornea. Control samples did not exhibit dark repair. The ratio of sparsely labelled cells was similar in the epidermis and in the corneal epithelium, but was significantly higher in the corneal stroma and highest in the endothelium.

Animals↗
PubMed1980

[Limits and possibilities of ophthalmodynamometry and ophthalmodynamography in the diagnosis of carotid occlusion (author's transl)].

In 67 patients suspected to suffer from cerebral vascular disorders or arterial occlusive disease results of ophthalmodynamometry and ophthalmodynamography were compared with those of carotid angiography. Especially in patients with angiographically substantiated carotid stenosis the results of ODM and ODG were extraordinarily poor. The findings of ODM and ODG proved to be non indicative in 29 out of 35 cases. The authors discuss the possible reasons of these disappointing results.

Brain Ischemia↗
PubMed1980

The effect of acetyl-salicylic-acid pretreatment on re-formed aqueous humor. A study of intracapsular lens extraction in the human eye.

Aqueous humor (a.h.) was sampled in 67 patients operated on for cataract by intracapsular lens extraction at the start and finish of surgery. Twenty eight of these patients received pretreatment with acetyl-salicylic acid (ASA), to a total of 2.5 g, beginning 18 hours prior to surgery. A serum sample was drawn immediately following the operation from 55 patients. Using radial immunodiffusion, albumin and IgG were measured in primary and secondary a.h. and in serum. The group pretreated with ASA showed a significantly smaller relative increase of IgG (P < 0.05). The increase of the proteins, however, showed no dependence on their serum-level and the only side-effect of the treatment was an unexplained rise in serum-albumin. The possible clinical application of patients with a high risk of postoperative complications is briefly summarized.

Aqueous Humor↗
PubMed1980

Cyclocryokoagulation. Sequelae of induced alterations and effect of a prostaglandin-inhibitor on the breakdown of the blood-aqueous barrier.

Histopathological changes following cyclocryokoagulation in a group of rabbits pretreated with acetylsalicyclic acid (ASA) were studied and compared with similar changes in a control group. As observed clinically, macroscopical and histological differences were only noted in the early phase after the procedure. In this phase, all operated eyes revealed an immense vascular response with edema, exudation and extravasation of blood cells. The treated animals, however, showed some reduction of tissue edema than the animals not treated. At a later stage there is an necrosis of the stroma with reduction of small vessels and tissue cells. The reparative process, tending to restore both epithelial and stromal lesions, begins already in the 3rd. week after cyclocryokoagulation. Neither the epithelial nor the stromal alterations differed significantly in any of the operated eyes at this stage. This indicates that ASA mainly affects an obviously prostaglandin-mediated acute vascular response. The disruption of the epithelial barrier may be aggravated by the subsequent stromal alterations, but does not seem to be influenced by ASA.

Animals↗