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Biomedical subjects

G Goessens

Publications and source records attributed to G Goessens.

At least 55 records · Page 3Linked to original sources
PubMed1984

Nuclear bodies in mouse lymphoid cells stimulated by lipopolysaccharide.

C57 BL/6J mouse spleen lymphocytes have been stimulated by a polyclonal mitogene, the lipopolysaccharide of E. coli (LPS). Depending on the LPS concentration, two pathways of B lymphocyte differentiation can be obtained. At low dose, the population is mainly composed of blast cells (85%) and at a high dose, the latter transforms into plasma cells (80%). Four types of nuclear bodies have been distinguished and quantitatively studied at several stages of cell differentiation. Only the simple nuclear bodies type A, which could be related to the nuclear matrix, show quantitative modifications in small lymphocytes. Connections between granular nuclear bodies (type D) and nucleolar material have been observed. Some granular nuclear bodies exhibit a morphological zone similar to the nucleolar fibrillar centre as well as fibrillar and granular components. Autoradiographic studies indicate that the granular nuclear bodies contain RNA synthesized elsewhere in the nucleus and that this RNA subsequently migrates to the cytoplasm. Furthermore connections between granular nuclear bodies and chromatin have also been observed.

Animals↗
PubMed1981

[Study of human synovial cells in culture].

Normal human synovial cells are cultivated in vitro; they actively multiply and fibroblast-like cells with structural characteristics of intermediate synoviocytes are obtained. In view of analyzing some functional aspects of this cell line, their endocytotic capacities have been studied. They also have been cultivated in a medium deprived of serum; a new cell type develops ("dendritic cells"). The latter are analyzed (cell division, endocytosis). As a comparison, human pulmonary fibroblasts (W I 38 line) and mouse macrophages are cultivated and studied under the same experimental conditions.

Culture Techniques↗
PubMed1979

Localisation of nucleolus-organizing regions in interphase cells.

A technique based on the use of silver solutions, which selectively stains the nucleolus-organising regions (NORs) in chromosomes, was applied to interphase Ehrlich tumour cells. The results indicate that nucleolar fibrillar centres correspond to the NORs.

Animals↗
PubMed1976

The nucleolar fibrillar centres in various cell types in vivo or in vitro.

Nucleoli were studied in chick fibroblasts cultured in vitro, under normal or under experimental conditions, and in several mammalian cell types in vivo. All these cells frequently contain nucleoli with fibrillar centres. The nucleolar fibrillar centres are composed of fibrous material of low electron density and are always intimately associated with the dense fibrillar component. Their morphology is very similar to that analysed cytochemically in Ehrlich tumour cells. It therefore appears that they could be related to the nucleolar organizers as suggested in Ehrlich tumour cells.

Animals↗
PubMed1976

Comparative ultrastructural study of the effects of serum-free medium and dibutyryl-cyclic AMP on newborn rat astroblasts.

Modifications of cell shape induced in cultured newborn rat astroblasts by serum deprivation or dibutyryladenosine 3'-5' monophosphate (dBcAMP) are described. Serum suppression modifies the adhesitivity of the cells to the substrate, but this modification is not consistent with a true differentiation. The main ultrastructural feature of dBcAMP-treated astroblasts is the presence of an extensive system of 90 A microfilaments, while control cells are relatively devoid of these structures.

Animals↗
PubMed1975

Mode of action of amphotericin B on chick embryo fibroblasts and on mouse Ehrlich tumour cells: a cytological and cytochemical analysis.

Chick embryo fibroblasts cultivated in vitro and Ehrlich ascites tumor cells (in vivo or in vitro) have been treated with amphotericin B. Cell multiplication is strongly inhibited. Large clear zones appear in the fibroblast nucleoi (phase-contrast and electron-microscope observations). Many treated fibroblasts and tumour cells have a high DNA content (pre-mitotic or polyploid level; measurements by cytophotometry). However, the RNA content (cytophotometry) and the total protein content (cytophotometry and micro-interferometry) are relatively low in the tumour cells. As shown by autoradiography, DNA synthesis is active but RNA synthesis and, in some cases, protein synthesis are inhibited. Due to this unbalanced growth, the cells cannot divide.

Amphotericin B↗