Search PubMed⌕ Search

Biomedical subjects

G Giron

Publications and source records attributed to G Giron.

At least 37 records · Page 2Linked to original sources
PubMed1978

G6PD Vientiane: a new glucose-6-phosphate dehydrogenase variant with increased stability.

A new G6PD variant, called G6PD Vientiane, has been discovered in a patient from Laos. The characteristics of this variant are: mild enzyme deficiency (about 50% of the normal activity) in the granulocytes and the red cells, with normal G6PD-related antigen concentration; increased stability; normal Km glucose 6-phosphate and NADP+; increased inhibition constant by NADPH; decreased inhibition by ATP; slightly increased utilization of the substrate analogue; abnormal pH curve, with maximum activity at pH 9.5; slightly reduced starch gel electrophoretic migration. The implications of the molecular stability of a deficient mutant variant are discussed.

Adult↗
PubMedDate not supplied

The role of manual acupuncture and morphine administration on the modulation of capsaicin-induced edema in rat paw. A blind controlled study.

Neurogenic inflammation, includes vasodilation, the increase of vasal permeability and edema due to the release of neuromediators from nerve endings. A modulation of the release of neurotransmitters, from nerve endings and neurogenic inflammation related phenomena, was observed with the administration of drugs or of neurotransmitters inhibiting the release of neuropeptides from capsaicin-sensible neurons, among which morphine and other endogenous agonist opioids seem to be effective. The aim of this study is to verify the effect of acupuncture on the peripheral release of Substance P due to the injection of capsaicin in rat paw. The study was carried out on 44 male Sprague-Dawley divided in 3 groups according to the treatment they were submitted to. Group 1 [20 rats] were controls; group 2 [10 rats] was received intraperitoneal morphine chloridrate 5 mg/Kg; group 3 [14 rats] stimulated with manual acupuncture. Morphine is effective in controlling inflammatory response according to other Authors' results. Also manual acupuncture is effective in controlling the edematogenic response in a statistically significant way of comparison with controls.

Acupuncture Therapy↗
PubMedDate not supplied

The effects of parachlorophenylalanine and naloxone on acupuncture and electroacupuncture modulation of capsaicin-induced neurogenic edema in the rat hind paw. A controlled blind study.

OBJECTIVE: The aim of this work was to study the effect of pre-treatment with parachlorophenylalanine (PCPA) and posttreatment with naloxone on the modulating action on neurogenic inflammation of manual acupuncture and low intensity (5 mAmp), low frequency (5 Hz) electroacupuncture (EA). METHODS: Edema was induced by the subcutaneous administration of 50 micrograms capsaicin in rat paws. Pre-treatment with intraperitoneal PCPA was given for 3 days: 200 mg/Kg on the first day and 100 mg/Kg on the second and third days. Naloxone (1 mg/Kg) was administered at the end of the stimulation. RESULTS: The results show that naloxone and PCPA reduce the anti-edema effect of both manual acupuncture and EA. Combined administration of the two drugs completely eliminated the effect of manual acupuncture, and decreased but did not abolish the effect of electroacupuncture. CONCLUSION: These results indicate that both the opioid and the serotonergic inhibitory control systems are involved in the modulating action of acupunctural stimulation on neurogenic inflammation.

Animals↗
PubMedDate not supplied

Demand and availability of Intensive Care beds. A study based on the data collected at the SUEM 118 Central of Padua from October 1996 to December 2001.

AIM: This study aims to evaluate the management of intensive care beds according to the demands received by the SUEM 118 of Padua. It has been carried out by examining the reports drawn up by SUEM physicians from October 1996 to December 2001. The study rated the number of patients for whom an admission to the Intensive Care Unit (ICU) was required, according to the specific clinical situation at the moment of the request. A secondary objective was to evaluate if the critically ill patients had been admitted and treated in the most appropriate medical facility. METHODS: The research is based on 7 087 reports concerning a population of adult and pediatric patients for whom an ICU bed was required in the period previously mentioned. For each report, it analyses the following data (keeping them anonymous): date of demand, main pathology and severity of clinical condition, sex and age, provenence and destination. RESULTS: Even though the number of annual demands for an ICU bed made to SUEM Central 118 has remained unchanged (approximately 1 350 per year), the number of beds made available in the operating rooms of the Hospital of Padua markedly increased. What has been experienced so far, and the data collected in this study has revealed, was that the requests for an intensive treatment for the overall population (hospitalized and non hospitalized) increased disproportionally in relation to the availability of ICU beds. In fact, the total number of hospitalizations in the different ICUs rose steadily year by year (from 3 495 in 1996 to 4 640 in 2001). CONCLUSION: The Hospital of Padua is a landmark center for patients who need specialized treatment. It is therefore important to increase the assistance and safety standards of its ICUs. In recent years there has been a great need for specialized ICUs either for more aggressive procedures (neurosurgical, cardiosurgical, respiratory, cardiologic, etc.) or for the increased use of adequate and invasive treatment for advanced diseases. The available resources of ICU beds should be more rationally distributed between the peripheral and the Regional Hospitals, since the activation of an ICU bed in the operating theatre is a valid, transient option.

Adolescent↗
PubMedDate not supplied

Cerebral tryptophan metabolism in humans in relation to malignant pain.

The authors investigated the cerebral metabolism of tryptophan in patients suffering from malignant pain by means of CSF dosage of tryptophan (Trp), 5-hydroxytryptophan (5-HTP), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA). The level of 5-HIAA in patients with pain was 66.48 +/- 13.67 ng/ml, while in those without pain was 25.05 +/- 13.25 ng/ml; the difference was statistically significant, p = 0.001. Trp, 5-HTP and 5-HT levels did not register significant differences in the two groups of patients, although a tendency to lower values was seen in patients with pain, supporting the hypothesis of increased turnover of this metabolic pathway in cancer patients. A statistically significant inverse correlation was also found between cerebral Trp levels and pain levels measured on the Scott-Huskisson visual analogue scale. The data obtained confirm the importance of the cerebral serotoninergic pathway in pain modulation and the interest which CSF analysis may have for the assessment of patients suffering from pain.

Aged↗
PubMedDate not supplied

Auditory and somatosensory evoked potentials in brain-dead patients.

The short latency evoked potentials, allowing to assess the brain stem's function, can supply useful information in the diagnosis of Brain Death (BD). 15 BD patients were submitted to the auditory brain stem response (ABR); in 7 cases somatosensory evoked potentials from the medial nerve (SEP) were also recorded. The ABR was absent in 11 cases (73.3%), while in 3 cases only the I wave was present (20%); in one case the low-voltage I-V waves were present. Regarding the SEP, in 3 cases (42.9%) only the N9-N13 and P9-P13 waves were present, while in another 3 cases (42.9%) a N13/P13 dissociation was observable. In the remaining case, which presented a still reproducible I-V interval, the SEP was normal, thus excluding the diagnosis of BD. The ABR and the SEP, which are not roughly influenced by general anaesthetics and sedatives, are thus helpful in diagnosing BD. The SEP seems able to supply useful information more frequently than the ABR, but their combined use can guarantee maximum security of excluding false positives.

Adolescent↗