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Biomedical subjects

G Giammanco

Publications and source records attributed to G Giammanco.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

A multicentric study for serotyping and susceptibility to antibiotics of pneumococci in Italy.

A multicentric study of the incidence of pneumococcal serotypes was recently carried out in five different geographical areas in Italy. Two hundred and sixty-seven strains were typed using the quellung reaction with monospecific antisera, but only 28 of these were isolated directly from the site of infection. The most frequent serotypes were 19, 6, 3, 9, 4, 1 and 18 without remarkable differences regarding the area, age of patients and site of isolation. It was found that 60.3% of the serotypes isolated from all sources and 67.8% of those isolated from the sites of infection are contained in the currently used antipneumococcal vaccine. Antibiotic resistance was found versus aminoglycosides (34.9%), tetracycline (28.3%), chloramphenicol (5.2%) and penicillin (3.3%). Cefoxitin appeared to be the antibiotic to which the pneumococci were most susceptible. Only two types, 19 and 16, resulted resistant to all the antibiotics.

Anti-Bacterial Agents↗
PubMedDate not supplied

Detection of aminopeptidases in Enterobacteriaceae by three simple chromogenic tests.

The action of 907 strains belonging to the various genera and species of the family Enterobacteriaceae were assayed on three chromogenic substrates used for the detection of aminopeptidases. The observed patterns of reactions may be useful for the biochemical identification of some serovars and biovars, particularly in the genus Shigella.

Aminopeptidases↗
PubMedDate not supplied

Epidemiological markers of Shigella sonnei infections: R-plasmid fingerprinting, phage-typing and biotyping.

In 1980, the number of Shigella sonnei strains isolated in Sicily increased markedly. Approximately 80% of the isolates belonged to phage-type 3 and showed the same antibiotic resistance pattern, suggesting that an epidemic had been going on for several months. Plasmid analysis of strains isolated at various times in different places supported this view. Agarose gel electrophoresis of plasmid preparations from seven selected phage-type 3 isolates showed the presence of two plasmids, of 80 megadaltons (Mdal) and 55 Mdal, respectively. In addition, all but one harboured a 2.8-Mdal plasmid, while a 30-Mdal and a 47-Mdal plasmid were, respectively, present in two other isolates. The 80-Mdal plasmid was self-transmissible to Escherichia coli K12, which acquired "en bloc" the resistance patterns of the donor strains. All the self-transmissible R plasmids fell into the incompatibility group I1 and showed a similar endonuclease cleavage pattern. An S. sonnei strain which was isolated during the same period, but did not belong to phage-type 3, exhibited a totally different plasmid pattern. We can conclude that phage-typing and R-plasmid characterization (i.e. incompatibility group, molecular weight and endonuclease cleavage pattern) represent the most reliable methods for epidemiologic study of S. sonnei isolates.

Anti-Bacterial Agents↗